The influence of ethylene in plant tissue culture

Biddington, N. L.

doi:10.1007/bf00024072

Cited by 211 publications

(123 citation statements)

References 103 publications

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“…Although Ag ϩ inhibited the IAA response, treatments with the ethylene biosynthesis inhibitors AVG and CoCl 2 -blocking the ACC synthase and ACC oxidase activities, respectively-yielded conflicting data. Results from experiments using such inhibitors should be interpreted with caution (20,21). Clearly, further studies are required to determine the role of ethylene in the IAA-induced elongation.…”

Section: Discussionmentioning

confidence: 99%

Ethylene can stimulate Arabidopsis hypocotyl elongation in the light

Smalle

Haegman

Kurepa

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

276

213

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Ethylene inhibits hypocotyl elongation in etiolated Arabidopsis seedlings. However, when Arabidopsis was grown in the light in the presence of ethylene or its precursor 1-aminocyclopropane-1-carboxylic acid (ACC), a marked induction of hypocotyl elongation occurred. This resulted from an increase in cell expansion rather than cell division. The effects of ethylene and ACC were antagonized by the ethylene action inhibitor Ag ؉ . The elongation response was absent or weakened in a set of ethylene-insensitive mutants (etr1-3, ein2-1, ein3-1, ein4, ain1-10, ein7). With the exception of ein4, the degree of inhibition of hypocotyl elongation was correlated with the strength of the ethylene-insensitive phenotype based on the triple response assay. In addition, the constitutive ethylene response mutant ctr1-1, grown in the light, had a longer hypocotyl than the wild type. Exogenous auxin also induced hypocotyl elongation in light-grown Arabidopsis. Again, the response was abolished by treatment with Ag ؉ , suggesting that ethylene might be a mediator. The results showed that, depending on light conditions, ethylene can induce opposite effects on cell expansion in Arabidopsis hypocotyls.Plant cell expansion is thought to be controlled by the orientation of cortical microtubules in combination with both the extensibility of the cell wall and the turgor pressure inside the cell (1, 2). These processes are under control of light and phytohormones. Ethylene can both promote and inhibit cell growth depending on plant species and cell type (3). In Arabidopsis, it was found to inhibit cell expansion (4, 5). Hence, Arabidopsis plants treated with ethylene, as well as a mutant displaying constitutive ethylene responses (ctr1), show a severe growth inhibition throughout development. Roots and inflorescences are short and leaves remain unexpanded. In etiolated Arabidopsis seedlings, ethylene prevents hypocotyl elongation (6). Recently, with the cloning and characterization of the Arabidopsis HOOKLESS1 (HLS1) gene, it has been shown that ethylene can also promote cell elongation (7). Specific cells in the apical hook of etiolated seedlings are induced to elongate with differential growth and hook curvature as a result. HLS1 is thought to control growth via regulation of transport or chemical modification of auxin. Other examples of a cross-talk between the ethylene and auxin pathways have been found. Most auxin-resistant mutants appeared to be also ethylene insensitive (8). Moreover, studies with aux1 suggested that ethylene sensitivity is regulated by auxin (9). Current molecular-genetic approaches shed a new light on previous physiological evidence for a complex interplay between these two hormones (10, 11).Most ethylene mutants in Arabidopsis have been identified using the triple response (12). Analysis of the ethyleneinsensitive mutants etr1 (6), ein2 (13), and ain1 (14) demonstrated that the effects of this class of mutations are not restricted to the etiolated seedling stage, but are also observed throughout the life c...

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Section: Discussionmentioning

confidence: 99%

Ethylene can stimulate Arabidopsis hypocotyl elongation in the light

Smalle

Haegman

Kurepa

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

276

213

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“…Among the most commonly used are aminoethoxyvinylglycine (AVG) and aminooxyacetic acid (AOA), both inhibiting 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and cobalt ions that inhibit the conversion of ACC to ethylene (Biddington 1992). Another inhibitor widely used is the silver ion, either as nitrate or as more mobile thiosulphate (STS), which excels in ethylene action inhibition (Beyer 1976).…”

Section: Ethylene Inhibitorsmentioning

confidence: 99%

Adventitious rooting of conifers: influence of physical and chemical factors

Ragonezi

Klimaszewska

Castro

et al. 2010

Trees

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In conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from controlled seed orchard pollinations. This can be achieved, in some species, either through rooted cuttings or rooted microshoots, the latter regenerated through tissue culture in vitro. Thus far, both strategies have been used but often achieved limited success mainly because of difficult and inefficient rooting process. In this overview of technology, we focus on the progress in defining the physical and chemical factors that help the conifer cuttings and microshoots to develop adventitious roots. These factors include plant growth regulators, carbohydrates, light quality, temperature and rooting substrates/media as major variables for development of reliable adventitious rooting protocols for different conifer species.

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“…Entre os compostos tóxicos que se acumulam no interior dos frascos, utilizados em crescimento de cultura, o gás etileno tem sido o foco, pois possui papel importante no crescimento e desenvolvimento in vitro (BIDDINGTON, 1992). Esse regulador de crescimento induz a abscisão foliar e tem-se apresentado como um dos maiores problemas para o cultivo in vitro.…”

Section: Introductionunclassified

Respostas morfofisiológicas in vitro de plântulas de Anadenanthera colubrina (Vell.) Brenan var. cebil (Griseb) Altschul

Nepomuceno

Rios²,

Queiroz³

et al. 2009

Rev. Árvore

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das respostas morfofisiológicas das plântulas submetidas a várias condições de cultivo. As sementes foram desinfestadas e inoculadas em placas de Petri contendo papel germtest previamente esterilizado e umedecido com água estéril. As placas ficaram no escuro por dois dias até que ocorresse a germinação das sementes e, em seguida, transferidas para tubo de ensaio contendo meio de cultura WPM. Foram avaliados três tipos de fechamentos dos tubos de ensaio: filme de PVC, tampa plástica sem filme de PVC e tampão de algodão. No segundo experimento, o meio de cultura foi suplementado com carvão ativado (0,0; e 82,2 mM) e diferentes concentrações de sacarose (0,0; 29,21; 58,43; e 87,64 mM). Ao final de 30 dias, observaramse efeitos altamente significativos dos tipos de fechamento sobre todas as variáveis analisadas: comprimento da parte aérea (CPA), número de folhas (NF), número de pinas (NP), matéria seca da parte aérea (MSPA), abscisão foliar (AF), comprimento da raiz (CR), matéria seca da raiz (MSR) e número de raízes secundárias (NRS). A AF foi reduzida em 7,3 vezes, quando os tubos de ensaio foram fechados com tampão de algodão em relação ao fechamento com filme de PVC. Os maiores valores de CPA (102,5 mm), NF (3,0), CR (194,7 mm) e NRS (47,88) foram obtidos com 29,21 mM de sacarose; a MSPA (59,1 mg), quando utilizou 58,43 mM e MSR (33,37 mg), com 87,64 mM de sacarose. O carvão ativado apresentou efeito significativo apenas em NRS, com maior valor (45,79) na presença desse composto.Palavras-chave: Aeração, sacarose e carvão ativado. (0,0 and 82,2mM) and different sucrose concentrations (0,0; 29,21; 58,43; 87,64mM IN VITRO MORPHOPHYSIOLOGICAL ANSWERS OF THE SEEDLINGS OF Anadenanthera colubrina (Vell.) Brenan var. cebil (Griseb) Altschul

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The influence of ethylene in plant tissue culture

Cited by 211 publications

References 103 publications

Ethylene can stimulate Arabidopsis hypocotyl elongation in the light

Ethylene can stimulate Arabidopsis hypocotyl elongation in the light

Adventitious rooting of conifers: influence of physical and chemical factors

Respostas morfofisiológicas in vitro de plântulas de Anadenanthera colubrina (Vell.) Brenan var. cebil (Griseb) Altschul

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