The Influence of Environmental Temperature and Substrate Initial Moisture Content on Aspergillus Niger Growth and Phytase Production in Solid-State Cultivation

Carter, Seaborn B.; Nokes, Sue E.; Crofcheck, Czarena

doi:10.13031/2013.16073

Cited by 7 publications

(1 citation statement)

References 13 publications

(14 reference statements)

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“…Previous studies have identified Rhizopus oligosporus (Hsieh et al, 2007;Sparringa & Owens, 1999), Aspergillus sp. (Carter et al, 2004;Chang et al, 2011), Rhizopus oryzae (Liao et al, 2008) as potential fungal strains for GlcN production. These studies have evidently shown the high potentiality of the biological production of GlcN and are worth further exploration.…”

Section: Introductionmentioning

confidence: 99%

Enhanced glucosamine production from Aspergillus oryzae NCH-42 via acidic stress under submerged fermentation

Chew

Lin

et al. 2021

CyTA - Journal of Food

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Glucosamine (GlcN) has been widely used to treat osteoarthritis. However, GlcN derived from crustacean shells has several drawbacks, and it is not vegan-friendly. Here, we produced GlcN from the biomass of Aspergillus oryzae NCH-42 and investigated the effects of environmental factors (e.g. carbon and nitrogen sources, temperature, and pH) on the GlcN production of the fungal biomass. The acidic stress resulted in significantly higher GlcN content in the cell wall of A. oryzae NCH-42. Moreover, the SEM examination revealed that the mycelial structure grown at pH 2.5 was thick and sturdy, compared to that at neutral pH conditions. The final yield of GlcN could be enhanced by up to 7.75-fold by cultivating at modified PDB with an initial pH of 2.5 at 30°C for 4 days. In this study, the GlcN content of A. oryzae biomass could reach up to 0.31 g/g biomass, indicating its high potentiality for GlcN production. Aumento de la producción de glucosamina a partir de Aspergillus oryzae NCH-42 mediante estrés ácido en fermentación sumergidaLa glucosamina (GlcN) se ha utilizado ampliamente para tratar la artrosis. Sin embargo, aquella que deriva de los caparazones de crustáceos presenta varios inconvenientes y no es apta para veganos. En el presente estudio produjimos GlcN a partir de la biomasa de Aspergillus oryzae NCH-42 e investigamos los efectos de los factores ambientales (por ejemplo, las fuentes de carbono y nitrógeno, la temperatura y el pH) en la producción de GlcN de la biomasa fúngica. El estrés ácido dio lugar a un contenido de GlcN significativamente mayor en la pared celular de A. oryzae NCH-42. Además, el examen SEM permitió comprobar que la estructura micelial cultivada a pH 2.5 era gruesa y robusta, en comparación con la cultivada en condiciones de pH neutro. El rendimiento final de GlcN se pudo multiplicar hasta por 7.75 al cultivarla en PDB (caldo de dextrosa de papa [patata]) modificado con un pH inicial de 2.5 a 30°C durante 4 días. En este estudio, el contenido de GlcN de la biomasa de A. oryzae alcanzó hasta 0.31 g/g de biomasa, lo que indica su alta potencialidad para producir GlcN.

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Section: Introductionmentioning

confidence: 99%

Enhanced glucosamine production from Aspergillus oryzae NCH-42 via acidic stress under submerged fermentation

Chew

Lin

et al. 2021

CyTA - Journal of Food

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Microbial production of glucosamine and N-acetylglucosamine: advances and perspectives

Liu

Shin

et al. 2013

Appl Microbiol Biotechnol

113

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Glucosamine (GlcN), an amino sugar, is a compound derived from substitution of a hydroxyl group of a glucose molecule with an amino group. GlcN and its acetylated derivative, N-acetylglucosamine (GlcNAc), have been widely used in food, cosmetics, and pharmaceutical industries and are currently produced by acid hydrolysis of chitin (a linear polymer of GlcNAc) extracted from crab and shrimp shells. Microbial fermentation by filamentous fungi or recombinant Escherichia coli, as an alternative method for the production of GlcN and GlcNAc, is attracting increasing attention because it is an environmentally friendly process. Although the microbial production of GlcN and GlcNAc is hampered by low yield and high production cost, considerable advances have been made in recent years. Here we review the applications, commercial market, and production of GlcN and GlcNAc, with emphasis on the metabolic and process engineering strategies used to improve GlcN and GlcNAc production by recombinant microbes.

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Enhanced Glucosamine Production with Actinomucor elegans Based on Stimulating Factor of Methanol

Liang

et al. 2014

Indian J Microbiol

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Glucosamine (GlcN) is a major and valuable component in the cell wall of fungi. In this study, the cell wall was treated via a two-stage alkali and acid process, and chitin and chitosan were fully deacetylated, partially depolymerized, and converted to GlcN oligosaccharides. Then, the oligosaccharides were analyzed by high performance liquid chromatography. The influences of Actinomucor elegans on GlcN production in a flask culture were investigated to achieve an optimum yield of GlcN. The experimental result showed that cultivation in condition of pH 6.0, 100 mL working volume (500 mL flask), 10 % (v/v) inoculum concentration, at 28 °C and 200 rpm for 6 days yielded highest dry cell weight (DCW) which was 23.43 g L(-1), with a GlcN concentration of 5.12 g L(-1). Methanol as stimulating factor was found to exert the best effect in concentration of 1.5 % (v/v). With addition of methanol into medium, the DCW increased from 23.69 to 32.42 g L(-1), leading to maximum GlcN concentration of 6.85 g L(-1) obtained. Here, the methanol addition may be useful for industrial production of GlcN, and may also be meaningful for the production of other fine chemicals by filamentous fungi.

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The Influence of Environmental Temperature and Substrate Initial Moisture Content on Aspergillus Niger Growth and Phytase Production in Solid-State Cultivation

Cited by 7 publications

References 13 publications

Enhanced glucosamine production from Aspergillus oryzae NCH-42 via acidic stress under submerged fermentation

Enhanced glucosamine production from Aspergillus oryzae NCH-42 via acidic stress under submerged fermentation

Microbial production of glucosamine and N-acetylglucosamine: advances and perspectives

Enhanced Glucosamine Production with Actinomucor elegans Based on Stimulating Factor of Methanol

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