The influence of different sample preparation on mechanical properties of human iliotibial tract

Fischer, B.; Kurz, Sascha; Höch, Andreas; Schleifenbaum, Stefan

doi:10.1038/s41598-020-71790-5

Cited by 10 publications

(7 citation statements)

References 25 publications

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“…This complex subsequently interacts with the PO 4 3- and forms critical size nuclei, which are useful for HA crystal formation. 24,25 Moreover, the compressive strength of the BHA-GEL scaffold is the closest to that of the human cortical bone (100–230 MPa), and slightly higer than the human bone tensile strength reported by Fischer et al 39 This compressive strength of BHA-GEL was also the closest to the rat femur bone, which was reported as 126.6 ± 19.7 and 167.3 ± 42.2 MPa for metaphyseal and diaphyseal specimens, respectively. 37 These hard characteristics of BHA-GEL scaffolds may prevent the premature degradation of the scaffold in vivo.…”

Section: Discussionmentioning

confidence: 65%

Bovine hydroxyapatite-based scaffold accelerated the inflammatory phase and bone growth in rats with bone defect

Gani

Budiatin

Shinta

et al. 2023

Journal of Applied Biomaterials & Functional Materials

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Hydroxyapatite (HA) is a biomaterial widely used to treat bone defect, such as due to traffic accident. The HA scaffold is obtained from synthetic HA or natural sources, such as bovine hydroxyapatite (BHA). This study aims to compare the characteristics and in vivo performance of BHA-based and HA-based scaffolds. For this purpose, the scaffold was formulated with gelatin (GEL) and characterised by SEM-EDX, FTIR and mini autograph. The defect model was carried out on the femur area of Wistar rats classified into three animal groups: defect, HA-GEL and BHA-GEL. Postoperatively (7, 14 and 28 days), the bone was radiologically evaluated, and stained with haematoxylin–eosin, anti-CD80 and anti-CD163. The BHA-GEL scaffold showed a regular surface and spherical particle shape, whereas the HA-GEL scaffold exhibited irregular surface. The BHA-GEL scaffold had higher pore size and compressive strength and lower calcium-to-phosphorus ratio than the HA-GEL scaffold. In vivo study showed that the expression of CD80 in the three experimental groups was not significantly different. However, the expression of CD163 differed significantly between the groups. The BHA-GEL group showed robust expression of CD163 on day 7, which rapidly decreased over time. It also showed increased osteoclasts, osteoblasts and osteocytes cell count that contributed to the integrity of the defect area. In conclusion, the BHA-based scaffold exhibited the desired physical and chemical characteristics that benefit in vivo performance versus the HA-based scaffold. Thus, the BHA-based scaffold may be used as a bone graft.

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Section: Discussionmentioning

confidence: 65%

Bovine hydroxyapatite-based scaffold accelerated the inflammatory phase and bone growth in rats with bone defect

Gani

Budiatin

Shinta

et al. 2023

Journal of Applied Biomaterials & Functional Materials

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“…Furthermore, in a previous study, the influence of the material sample condition on the determination of the material properties could be demonstrated [ 16 ]. As expected a priori, the best possible results were always obtained with fresh and unfixed specimens.…”

Section: Methodsmentioning

confidence: 99%

Approach to standardized material characterization of the human lumbopelvic system—Specification, preparation and storage

Kurz,

Gebhardt,

Grundmann

et al. 2023

PLoS ONE

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The complexity of the osseo-ligamentous lumbopelvic system has made it difficult to perform both, the overall preparation as well as specimen harvesting and material testing with a reasonable amount of time and personnel. The logistics of such studies present a hurdle for reproducibility. A structured procedure was developed and proved, which allows all necessary steps to be carried out reproducibly and in a reasonable time. This enables the extraction of 26 soft tissue, 33 trabecular and 32 cortical bone specimens from this anatomical region per cadaver. The integrity of the specimens remains maintained while keeping requirements within manageable limits. The practicability of the intended five-day specimen harvesting and testing procedure could be demonstrated on five test and two pre-test sequences. The intended minimization of physical, biological, and chemical external influences on specimens could be achieved. All protocols, instructions and models of preparation and storage devices are included in the supporting information. The high grade of applicability and reproducibility will lead to better comparability between different biomechanical investigations. This procedure proven on the human pelvis is transferable to other anatomical regions.

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“…Due to logistical constraints of the study, there was a delay between in vivo measurements and ex vivo testing; thus, tissue preservation was necessary. Based on various literature, freezing at −80 • C was chosen as the preferred method for preserving bulk tissue mechanical properties (29,31,48,49). The tissue was checked macroscopically for integrity but not microscopically, so it is possible that microscopic changes could have occurred 10.3389/fcvm.2022.884116 and were not detected.…”

Section: Limitations and Challengesmentioning

confidence: 99%

Characterization of main pulmonary artery and valve annulus region of piglets using echocardiography, uniaxial tensile testing, and a novel non-destructive technique

Sutherland

McEleney

Almeida

et al. 2022

Front. Cardiovasc. Med.

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Characterization of cardiovascular tissue geometry and mechanical properties of large animal models is essential when developing cardiovascular devices such as heart valve replacements. These datasets are especially critical when designing devices for pediatric patient populations, as there is often limited data for guidance. Here, we present a previously unavailable dataset capturing anatomical measurements and mechanical properties of juvenile Yorkshire (YO) and Yucatan (YU) porcine main pulmonary artery (PA) and pulmonary valve (PV) tissue regions that will inform pediatric heart valve design requirements for preclinical animal studies. In addition, we developed a novel radial balloon catheter-based method to measure tissue stiffness and validated it against a traditional uniaxial tensile testing method. YU piglets, which were significantly lower weight than YO counterparts despite similar age, had smaller PA and PV diameters (7.6–9.9 mm vs. 10.1–12.8 mm). Young’s modulus (stiffness) was measured for the PA and the PV region using both the radial and uniaxial testing methods. There was no significant difference between the two breeds for Young’s modulus measured in the elastic (YU PA 84.7 ± 37.3 kPa, YO PA 79.3 ± 15.7 kPa) and fibrous regimes (YU PA 308.6 ± 59.4 kPa, YO PA 355.7 ± 68.9 kPa) of the stress-strain curves. The two testing techniques also produced similar stiffness measurements for the PA and PV region, although PV data showed greater variation between techniques. Overall, YU and YO piglets had similar PA and PV diameters and tissue stiffness to previously reported infant pediatric patients. These results provide a previously unavailable age-specific juvenile porcine tissue geometry and stiffness dataset critical to the development of pediatric cardiovascular prostheses. Additionally, the data demonstrates the efficacy of a novel balloon catheter-based technique that could be adapted to non-destructively measure tissue stiffness in situ.

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The influence of different sample preparation on mechanical properties of human iliotibial tract

Cited by 10 publications

References 25 publications

Bovine hydroxyapatite-based scaffold accelerated the inflammatory phase and bone growth in rats with bone defect

Bovine hydroxyapatite-based scaffold accelerated the inflammatory phase and bone growth in rats with bone defect

Approach to standardized material characterization of the human lumbopelvic system—Specification, preparation and storage

Characterization of main pulmonary artery and valve annulus region of piglets using echocardiography, uniaxial tensile testing, and a novel non-destructive technique

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