The influence of cyclophosphamide on the process of priming for the secondary response

Finger, H; Emmerling, P; Brüss, E

doi:10.1007/bf01900264

Cited by 12 publications

(6 citation statements)

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“…2). This lack of any positive correlation between the degree of the secondary immune response and the priming for the tertiary immune reaction does not support the concept that memory cell production depends on antecedent antibody production [13,22], On the contrary, this finding correlates well with pre vious data obtained in cyclophosphamide-treated [8] and pertussis-treated [9] mice, which indicated that the mature hemolysin-producing cells have little to do with immunological memory.…”

Section: Discussionsupporting

confidence: 78%

Time Relationships between Injection of Antigen and Adjuvant

Finger¹,

Bartoschek²,

Emmerling³

1972

Pathobiology

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The influence of Bordetella pertussis was tested, both at the cellular and humoral levels, when the bacterial adjuvant was administered 2 days before the secondary immunization of mice with 4 x 10⁸ sheep erythrocytes. Under these experimental conditions, a significant suppression of the secondary response was induced, without influencing the process of priming for the tertiary immune response. From this it is suggested that B. pertussis cells act as non-specific proliferative stimuli, caused by the toxic damage of cells which are involved with the early steps of antibody formation.

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Section: Discussionsupporting

confidence: 78%

Time Relationships between Injection of Antigen and Adjuvant

Finger¹,

Bartoschek²,

Emmerling³

1972

Pathobiology

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“…The IgG antibody response is long-lived compared to the IgM response but declines with different kinetics depending upon the antigens used. For sheep red blood cells, for example, the IgG titer seems to drop much faster [3] than after immunization with haptenated proteins in adjuvants [4] or after viral infections [5].The level of this IgG response seems to be determined by the number of B and T helper cells that have been available and induced during priming in an antigen dose-dependent fashion. Antibody titers obtained after secondary immunizations usually reach much higher levels than after primary immunizations correlating with the presence of memory T helper and B cells at elevated frequencies (reviewed in [6,71).…”

Section: Introductionmentioning

confidence: 99%

Regulation of IgG antibody titers by the amount persisting of immune‐complexed antigen

et al. 1994

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Antigens normally induce an immunoglobulin (Ig)G response which stays at an elevated level for several weeks or months, constituting an important part of the immunological memory. This study investigated factors influencing the level of neutralizing IgG titers against a virus and shows that within the range tested it was independent of the number of initially available and potentially responding T helper and B cells, but was regulated by the amount of specific IgG-immune complexes forming depots of persisting antigen. These findings support the notion that the efficiency of vaccines in inducing long-lasting protective IgG is regulated predominantly by the amount of persisting (and presumably follicular dendritic cell-associated) antigen-antibody complexes.

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“…As already shown by L educ et al [16], active antibody synthesis is not a prerequisite for immunological memory. Although the process of priming for the secondary response was observed to be prevented by chloramphenicol in the serological studies of C rucheaud and C oons [4], other serological investigations revealed that the secondary response was not inhibited at all when the primary response was suppressed by 6-mercaptopurine [20] or chloramphenicol [22], Studies performed at the cellular level also showed that cyclophosphamidemediated suppression of the primary immune response did not actually prevent the process of priming for the secondary response [12]. In those experiments, the drug was given as 4 daily divided doses beginning 2 days before primary immunization.…”

Section: Discussionmentioning

confidence: 99%

“…Emyloying sheep red blood cells (SRBC) as an immunizing anti gen, the simultaneous injection of a sufficient dose of CY leads to almost complete suppression of the primary immune response in mice, both at the cellular and humoral levels [1,10]. As opposed to this, CY was not found to be capable of preventing the process of priming for the secondary immune response when given simultaneously with the erythrocyte antigen in young adult [12] and aged [6] mice. Although complete immunological tolerance to SRBC has been induced in mice when CY was given together with a tolerance-inducing sheep cell injection (6.4 x 109 SRBC), and when tolerance was maintained by weekly intraperitoneal (i.p.)…”

mentioning

confidence: 85%

Recovery of the Antibody-Forming Potential in Cyclophosphamide-Treated Mice, with Special Reference to the Influence of <i>Bordetella pertussis</i>

Elekes¹,

Finger²

1974

Pathobiology

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A single intraperitoneal injection of 4mg cyclophosphamide administered 2 days before the primary immunization of NMRI mice with 4 × 10⁸ sheep erythrocytes led to a significant suppression of the primary immune response to this antigen, both at the cellular and humoral levels. Unlike this, the secondary response elicited 4 weeks after primary antigenic stimulation was found to be increased. To a lesser degree, the inhibition of the primary immune response was only demonstrable when the drug was given as early as 4 days before priming, although at that time the maximum in both the reduction of the spleen weights and the blood leukocyte counts were found. Again, the secondary response of the cyclophosphamide-treated animals was found to be increased. Killed cells of Bordetella pertussis, given simultaneously with the erythrocyte antigen, were capable to overcome partly the drug-induced inhibition of the primary immune response, and to increase the process of priming for the secondary immune reaction. Furthermore, the B. pertussis vaccine effected accelerated increase in both the extremely suppressed spleen weights and blood leukocyte counts.

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The influence of cyclophosphamide on the process of priming for the secondary response

Cited by 12 publications

References 0 publications

Time Relationships between Injection of Antigen and Adjuvant

Time Relationships between Injection of Antigen and Adjuvant

Regulation of IgG antibody titers by the amount persisting of immune‐complexed antigen

Recovery of the Antibody-Forming Potential in Cyclophosphamide-Treated Mice, with Special Reference to the Influence of <i>Bordetella pertussis</i>

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