The influence of culture conditions on vasculitis and anaphylactoid shock induced by fungal pathogen Candida albicans cell wall extract in mice

β-mannosyl linkages inhibit CAWS arteritis by negatively regulating dectin-2-dependent signaling in spleen and dendritic cells

“…Significant chemical shifts in the CAWS727 spectrum at 4.84, 4.85 and 5.13, shown as open arrows in the spectra, were distinguished by 2D-NMR as specific peaks that were not found in the CAWS spectrum. The origin of these shifts was identified as b-linked mannosyl oligosaccharides from previous reports of NMR being used to analyze Candida mannan by Shibata et al 25,26 and Tada et al 21 These findings strongly suggested a significant increase in b-1,2-mannosyl linkages in CAWS727, and reactivity to the Candida antigenic factor sera, factor-5 and factor-6 was also proven (data not shown). In contrast, signals $5.05, shown as closed arrows, which include a-mannosyl residues, were significantly reduced in the CAWS727 spectrum.…”

“…It is also an immunochemical determinant of Candida spp 19 . Previous reports have demonstrated that the structure of mannan is modulated by various culture conditions 20 , such as temperature and pH, and also by various stresses 8,21,22 . The structure of mannan is key for the recognition of Candida by the host biodefense system and may be strictly regulated by its affinity for specific receptors.…”

Murine Model of Dextran Sulfate Sodium-induced Colitis Reveals Candida glabrata Virulence and Contribution of β-Mannosyltransferases

“…Paradoxically, ␤-glucan was recently shown to interact with ␤-Mans and to exhibit anti-inflammatory effects (48). In a model of vasculitis induced by C. albicans cell wall extracts, growth conditions favoring the expression of ␤-Mans decreased the proinflammatory potential (49). Thus, although mannan has long been recognized as a potent modulator of the host response, the repertoire of oligomannoses that are recognized differently by the immune system is complex (50,51) and varies depending on the growth conditions.…”

Dectin-2 Recognition of α-Mannans and Induction of Th17 Cell Differentiation Is Essential for Host Defense against Candida albicans

“…Preparation of CAWS and C. albicans Mannan CAWS was prepared from the C. albicans strain NBRC1385 in accordance with conventional methods (Tada et al, 2008). In brief, C. albicans strain NBRC1385 was cultured with 5 l of C-limiting medium in a glass incubator and cultured for 2 days at 27 C with air supplied at a rate of 5 l/min, rotating at 400 rpm.…”