The influence of culture conditions on the biosynthesis of secondary metabolites by Penicillium verrucosum Dierck

Elias, B.; Said, Suraia; Albuquerque, Sérgio de; Pupo, Mônica Tallarico

doi:10.1016/j.micres.2005.10.003

Cited by 27 publications

(15 citation statements)

References 21 publications

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“…The production of bioactive fungal compounds, influenced by cultivation conditions, usually occurs during the stationary phase (idiophase), and after fungal growth (tropophase) (Elias et al . ). Three phase pH changes were observed; in first phase, a significant decrease in pH within 144 h was observed due to the consumption of reducing sugar by Nigrospora sp.…”

Section: Discussionmentioning

confidence: 97%

Production and characterization of bioactive metabolites from piezotolerant deep sea fungusNigrosporasp. in submerged fermentation

et al. 2014

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Section: Discussionmentioning

confidence: 97%

Production and characterization of bioactive metabolites from piezotolerant deep sea fungusNigrosporasp. in submerged fermentation

et al. 2014

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“…The other evaluated fungal species of the genus Aspergillus play significant roles as model organisms in basic research and for manufacturing of a wide range of commercial enzymes (Pontecorvo et al, 1953; Baddley et al, 2003). T. flavus (formerly known as P. vermiculatum ) and the genus Penicillium have been reported to be producers of secondary metabolites with antimicrobial, antiprotozoal, antitumoral, or insecticidal activities (Freitas et al, 2002; Elias et al, 2006). N. crassa is able to degrade phenolic pollutants (Luke and Burton, 2001), and P. corylophilum is a plant pathogen and a parasite of fish (Gozlan et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activity against Filamentous Fungi of Ts1, a Multifunctional Toxin from Tityus serrulatus Scorpion Venom

et al. 2017

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Antimicrobial peptides (AMPs) are ubiquitous and multipotent components of the innate immune defense arsenal used by both prokaryotic and eukaryotic organisms. The search for new AMPs has increased in recent years, due to the growing development of microbial resistance to therapeutical drugs. In this work, we evaluate the effects of Tityus serrulatus venom (Tsv), its fractions and its major toxin Ts1, a beta-neurotoxin, on fungi growth. The fractions were obtained by ion-exchange chromatography of Tsv. The growth inhibition of 11 pathogenic and non-pathogenic filamentous fungi (Aspergillus fumigatus, A. nidulans, A. niger, A. terreus, Neurospora crassa, Penicillium corylophilum, P. ochrochloron, P. verrucosum, P. viridicatum, P. waksmanii, and Talaromyces flavus) was evaluated by quantitative microplate reader assay. Tsv (100 and 500 μg/well, which correspond to 1 and 5 mg/mL, respectively, of total soluble protein) was active in inhibiting growth of A. nidulans, A. terreus, P. corylophilum, and P. verrucosum, especially in the higher concentration used and at the first 30 h. After this period, fungi might have used Tsv components as alternative sources of nutrients, and therefore, increased their growth tax. Only fractions IX, X, XI, XIIA, XIIB (3 and 7.5 μg/well, which correspond to 30 and 75 μg/mL, respectively, of total soluble protein) and Ts1 (1.5, 3, and 6 μg/well, which correspond to 2.18, 4.36, and 8.72 μM, respectively) showed antifungal activity. Ts1 showed to be a non-morphogenic toxin with dose-dependent activity against A. nidulans, inhibiting 100% of fungal growth from 3 μg/well (4.36 μM). The inhibitory effect of Ts1 against A. nidulans growth was accompanied by fungistatic effects and was not amended by 1 mM CaCl2 or tetrodotoxin (46.98 and 93.96 μM). The structural differences between Ts1 and drosomycin, a potent cysteine-rich antifungal peptide, are discussed here. Our results highlight the antifungal potential of the first cysteine-containing scorpion toxin. Since Ts1 is a multifunctional toxin, we suggest that it could be used as a template in the design of engineered scorpion AMPs and in the search for new mechanisms of action of antifungal drugs.

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“…Firstly, fungal products not excreted are lost, and these types of products are often chemically different from those excreted. Secondly, fungi are known to adapt their metabolic pathways according to their environment [9], so they could produce a different variety of metabolites in other cultural conditions, such as in solid state fermentation (SSF) like agar surface fermentation (ASF).…”

Section: Introductionmentioning

confidence: 99%

Search for Hydrophilic Marine Fungal Metabolites: A Rational Approach for Their Production and Extraction in a Bioactivity Screening Context

et al. 2011

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In the search for bioactive natural products, our lab screens hydrophobic extracts from marine fungal strains. While hydrophilic active substances were recently identified from marine macro-organisms, there was a lack of reported metabolites in the marine fungi area. As such, we decided to develop a general procedure for screening of hydrophobic metabolites. The aim of this study was to compare different processes of fermentation and extraction, using six representative marine fungal strains, in order to define the optimized method for production. The parameters studied were (a) which polar solvent to select, (b) which fermentation method to choose between solid and liquid cultures, (c) which raw material, the mycelium or its medium, to extract and (d) which extraction process to apply. The biochemical analysis and biological evaluations of obtained extracts led to the conclusion that the culture of marine fungi by agar surface fermentation followed by the separate extraction of the mycelium and its medium by a cryo-crushing and an enzymatic digestion with agarase, respectively, was the best procedure when screening for hydrophilic bioactive metabolites. During this development, several bioactivities were detected, confirming the potential of hydrophilic crude extracts in the search for bioactive natural products.

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The influence of culture conditions on the biosynthesis of secondary metabolites by Penicillium verrucosum Dierck

Cited by 27 publications

References 21 publications

Production and characterization of bioactive metabolites from piezotolerant deep sea fungusNigrosporasp. in submerged fermentation

Production and characterization of bioactive metabolites from piezotolerant deep sea fungusNigrosporasp. in submerged fermentation

Antifungal Activity against Filamentous Fungi of Ts1, a Multifunctional Toxin from Tityus serrulatus Scorpion Venom

Search for Hydrophilic Marine Fungal Metabolites: A Rational Approach for Their Production and Extraction in a Bioactivity Screening Context

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