The influence of corticosteroid treatment on the OPG/RANK/RANKL pathway and osteocalcin in patients with pemphigus

Chmielnicka, Marlena; Woźniacka, Anna; Torzecka, Jolanta Dorota

doi:10.5114/pdia.2014.44016

Cited by 15 publications

(8 citation statements)

References 21 publications

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“…OPNt is a prominent component of the mineralized extracellular matrix of bone, 27 and the balance of RANKL/RANK and OPG plays a vital role in isphysiological bone remodeling and osteoclastogenesis modulation. 28 As OPG is a competitive inhibitor of RANK binding to RANKL, 29 the decreased expression of RANK in mimic group may attribute to the increased OPG. In the case of OC, which is related to glucose metabolism, bone metabolism, and fat mass, 30 there is a study showing that knockdown of miR-34b/c enhanced OC mRNA expression; 31 however, the relation between miR-34a and remains unknown, which needs future exploration.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA-34a alleviates steroid-induced avascular necrosis of femoral head by targeting Tgif2 through OPG/RANK/RANKL signaling pathway

Peng

Dong

et al. 2017

Exp Biol Med (Maywood)

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Impact statement miR-34a can alleviate SANFH through targeting Tgif2 and further regulating OPG/ RANK/RANKL signaling pathway, which can be used as a new theoretical basis for SANFH treatment. AbstractThe study aims to investigate the effect of microRNA-34a (miR-34a) targeting Tgif2 on steroid-induced avascular necrosis of femoral head (SANFH) by regulating OPG/RANK/ RANKL signaling pathway. SD rats were divided into normal control and model (RNAKL rat models) groups. The model group was further assigned into model control, negative control, miR-34a mimics and miR-34a inhibitors groups. QRT-PCR was applied to detect miR-34a, Tgif2, OPG, RANK and RNAKL mRNA expressions. Femoral head tissues were collected for Micro-CT scanning and HE staining. QRT-PCR and Western blotting were used to detect expressions of miR-34a, Tgif2, OPG, RANK, RANKL and Runx2, OPN and OC in bone tissues. Dual-luciferase reporter gene assay was used to testify the target relationship between miR-34a and Tgif2. Compared with the normal control group, the model group showed increased Tgif2, RANK and RANKL mRNA expressions, but decreased miR-34a and OPG mRNA expressions. Tgif2 mRNA expression was negatively correlated with miR-34a and OPG mRNA expressions. Micro-CT showed cystic degeneration of femoral head, with decreased bone volume/total volume (BV/TV), bone surface area/bone volume and trabecular number in the model control group compared with the normal control group. Compared with the model control group, the miR-34a mimics group showed increased BV/TV and trabecular thickness and Runx2, OPN and OC expressions, while the parameters decreased in the miR-34a inhibitors group. Compared with the normal control group, the other groups showed increased Tgif2, RANK and RANKL expressions but decreased miR-34a and OPG expressions. Compared with the model control group, Tgif2, RANK and RANKL expressions decreased and miR-34a and OPG expressions increased in the miR-34a mimics group, while the miR-34a inhibitors group had a reverse trend in contrast to the miR-34a mimics group. Tgif2 is a target gene of miR-34a. In conclusion, miR-34a can alleviate SANFH through targeting Tgif2 and further regulating OPG/RANK/RANKL signaling pathway.Keywords: MiR-34a, steroid-induced avascular necrosis of femoral head, osteoprotegerin, receptor activator of nuclear factor Kappa B, receptor activator of nuclear factor Kappa ligand, signaling pathway

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Section: Discussionmentioning

confidence: 99%

“…All SD rats were grouphoused (two rats/cage), with unlimited access to sterilized food and water in a standard diet. The cages were kept at [22][23][24][25][26] C and 37%-42% humidity on a reverse 12-h light/ dark cycle.…”

Section: Ethical Statementmentioning

confidence: 99%

MicroRNA-34a alleviates steroid-induced avascular necrosis of femoral head by targeting Tgif2 through OPG/RANK/RANKL signaling pathway

Peng

Dong

et al. 2017

Exp Biol Med (Maywood)

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“…It is generally agreed that OPG gene and OPG/RANKL pathway play an important role in vascular calcification [ 16 ]. OPG, a member of the tumor necrosis factor receptor superfamily, regulates bone resorption and widely exists in heart, lung and kidney [ 17 – 18 ]. Schoppet et al [ 19 ] confirmed that in vitro OPG overexpression inhibited VSMC calcification; the more severe the vascular calcification, the higher the OPG expression would be.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of genistein regulating the differentiation of vascular smooth muscle cells into osteoblasts via the OPG/RANKL pathway

Shen

Yuan

et al. 2017

Oncotarget

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ObjectiveThe present study aimed to investigate the mechanism of genistein, a tyrosine kinase inhibitor, regulating the differentiation of vascular smooth muscle cells (VSMCs) into osteoblasts via the OPG/RANKL (Osteoprotegerin/Receptor Activator of Nuclear Factor-κB Ligand) pathway.MethodsThe mouse VSMCs were isolated, purified and cultured. We constructed the LV5-Tnfrsf11b overexpression lentiviral vector and LV3-OPG-309 interference lentiviral vector. The OPG overexpression was induced and the growth of VSMCs infected with the lentiviral vector was observed. The VSMC calcification and control group were treated with different doses of genistein. The mRNA and protein expression levels of OPG, α-SM-actin (smooth muscle actin), ALP (alkaline phosphatase) and OPN (osteopontin) were detected in VSMCs after treatment using RT-PCR and Western Blot.ResultWe induced OPG overexpression and performed lentiviral vector infection of the VSMCs to suppress OPG expression, respectively, which was followed by treatment with genistein. The results showed that the relative expression of OPG was the highest in the VSMC calcification +genistein +OPG overexpression-inducing treatment group. It was the lowest in the VSMC calcification +OPG expression-suppressing treatment group. The relative expression of ALP was the highest in the VSMC calcification +OPG expression-suppressing treatment group, and the lowest in the VSMCs+genistein treatment group.ConclusionOPG gene plays an important regulatory role in the growth of VSMCs, by suppressing the calcification of VSMCs. Genistein could regulate the differentiation of VSMCs into osteoblasts via the OPG/RANKL pathway in a dose-dependent manner.

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“…RANKL is an essential adjustment factor of osteoclast proliferation, differentiation, activation, and survival. 29 …”

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confidence: 99%

Mechanism of enhanced antiosteoporosis effect of circinal–icaritin by self-assembled nanomicelles in vivo with suet oil and sodium deoxycholate

Jiang¹,

Li²,

Zhang³

et al. 2015

IJN

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Background Circinal–icaritin (CIT), one new active aglycone of Epimedium , can exert a beneficial effect on osteoporotic bone. However, its low bioavailability limits its clinical efficacy for the treatment of osteoporosis. Materials and methods In this paper, suet oil (SO) was used to improve the oral bioavailability of CIT and enhance its antiosteoporosis effect and absorption. After oral administration of CIT together with SO, the CIT and SO self-assembled into nanomicelles under the action of sodium deoxycholate (DOC) by bile secretion. The antiosteoporosis effects of the CIT-SO-DOC nanomicelles were evaluated in osteoporotic rats by bone mineral density, serum biochemical markers, bone microarchitecture, bone biomechanical properties, and related protein and gene expressions. We examined the bioavailability of CIT and its nanomicelles in vivo, and subsequently the nanomicelles were verified using transmission electron microscopy. Finally, we evaluated absorption across a rat intestinal perfusion model. Results Compared with CIT, in the CIT-SO groups, protein and messenger ribonucleic acid expressions of osteoprotegerin were increased, while expressions of receptor activator of nuclear factor-κB ligand in bone tissue were decreased; bone-turnover markers in serum of hydroxyproline, alkaline phosphatase, tartrate-resistant acid phosphatase 5b, and receptor activator of nuclear factor-κB ligand levels were decreased, while osteoprotegerin and osteocalcin levels were increased; and trabecular bone mass, microarchitecture, and bone biomechanical strength were enhanced. The relative bioavailabilities of CIT-SO high dosage, CIT-SO medium dosage, and CIT-SO low dosage (area under concentration–time curve [AUC] 0–∞ ) compared with that of raw CIT high dosage, CIT medium dosage, and CIT low dosage (AUC 0–∞ ) were 127%, 121%, and 134%, respectively. The average particle size of CIT-DOC was significantly decreased after adding SO ( P <0.01), and the intestinal permeability coefficients of CIT-SO-DOC nanomicelles in the duodenum, jejunum, ileum, and colon were all significantly improved ( P <0.01). Conclusion The increased antiosteoporosis effects and bioavailability of CIT-SO-DOC self-assembled nanomicelles were due to an increase in absorption of CIT by reducing the particle sizes of CIT. SO may be a practical oral carrier for antiosteoporosis drugs with low bioavailability.

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The influence of corticosteroid treatment on the OPG/RANK/RANKL pathway and osteocalcin in patients with pemphigus

Cited by 15 publications

References 21 publications

MicroRNA-34a alleviates steroid-induced avascular necrosis of femoral head by targeting Tgif2 through OPG/RANK/RANKL signaling pathway

MicroRNA-34a alleviates steroid-induced avascular necrosis of femoral head by targeting Tgif2 through OPG/RANK/RANKL signaling pathway

Mechanism of genistein regulating the differentiation of vascular smooth muscle cells into osteoblasts via the OPG/RANKL pathway

Mechanism of enhanced antiosteoporosis effect of circinal–icaritin by self-assembled nanomicelles in vivo with suet oil and sodium deoxycholate

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The influence of corticosteroid treatment on the OPG/RANK/RANKL pathway and osteocalcin in patients with pemphigus

Cited by 15 publications

References 21 publications

MicroRNA-34a alleviates steroid-induced avascular necrosis of femoral head by targeting Tgif2 through OPG/RANK/RANKL signaling pathway

MicroRNA-34a alleviates steroid-induced avascular necrosis of femoral head by targeting Tgif2 through OPG/RANK/RANKL signaling pathway

Mechanism of genistein regulating the differentiation of vascular smooth muscle cells into osteoblasts via the OPG/RANKL pathway

Mechanism of enhanced antiosteoporosis effect of&nbsp;circinal&ndash;icaritin by self-assembled nanomicelles&nbsp;in vivo with suet oil and sodium deoxycholate

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Mechanism of enhanced antiosteoporosis effect of circinal–icaritin by self-assembled nanomicelles in vivo with suet oil and sodium deoxycholate