The influence of chlorhexidine on the remineralization of demineralized dentine

Kim, Duck-Su; Kim, Jong-Ryul; Choi, Kyoung‐Kyu; Kim, Sun‐Young

doi:10.1016/j.jdent.2011.09.010

Cited by 48 publications

(67 citation statements)

References 45 publications

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“…The lack of augmentation (in caries-affected dentin treated with Zn-free amalgams) or the discrete growth (in caries-affected dentin treated with Zn-containing amalgams) of 579/959, 1043/959 and 1069/959 ( Table 3) makes sure that the caries-affected tissue is decreasing as result of dentin remineralization [5] . Images of specimens remineralized after removal of Zncontaining amalgam revealed fibrillar structures with significant topographical changes that suggest a preferential organization of mineral within the surface of collagen fibrils, in an intrafibrillar manner [48] . The remineralization procedure made the fibrils to exhibit a growing width of 73.05 nm (Figs.…”

Section: Resultsmentioning

confidence: 99%

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Osorio

Aguilera

Cabello

et al. 2016

J Raman Spectroscopy

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The aim of this study was to assess the mechanical and chemical performance of sound and caries-affected dentin (CAD), after Zn-free vs containing amalgam restorations placement and thermocycling. Dentin surfaces were studied by Atomic Force Microscopy (AFM) analysis for surface morphological characterization (including fibril diameter assessment), nano-indentation (to measure nano-hardness-Hi and modulus of Young-Ei), and single point Raman spectroscopy for chemical analysis. Measurements were performed before amalgam placement, after amalgam removal, and after 3 months of thermocycling (100,000cy/5 ºC and 55 ºC). Restorations increased both Hi and Ei at intertubular dentin of CAD. The highest values of Hi were achieved at intertubular dentin after restoring with Zn-containing amalgams. Remineralization of dentin was attributed to the increase of both amorphous and crystalline new mineral, as lower degrees of crystal imperfections in junction with crystal disorders, and improvement in structural stability of collagen were found. Higher presence of minerals were also confirmed after the decrease of fluoridated apatite and the increase of the total water content. Proteoglycans, lipids, and proteins, augmented in both sound and CAD, providing support for the mineral growing. The increase of bands assigned to vibration of carbonate calcium phosphate contributed to a decrease of crystallinity.

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Section: Resultsmentioning

confidence: 99%

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Osorio

Aguilera

Cabello

et al. 2016

J Raman Spectroscopy

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“…Dentine demineralization (other than that caused by acid etching for resin-dentine bonding) could result from an acid attack by bacteria and / or a food source (8) . This study adopts the remineralization of carious dentine rather than the surgical model of treatment.…”

Section: Discussionmentioning

confidence: 99%

“…2% chlorhexidine gluconate solution (Consepsis, Ultradent, USA) was applied on the pulpal floor in half of samples, left for 1 minute as recommended by Kim et al (8) , followed by a blast of compressed air for 5 seconds. Caution was taken not to over dry the preparation.…”

Section: Application Of 2% Chx Solutionmentioning

confidence: 99%

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Effect of Nanohydroxyapatite with and without Chlorhexidine on Remineralization of Carious Dentine at Different Intervals: An in vivo study

et al. 2016

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This invivo study was carried out to evaluate the effect of nanohydroxyapatite (n-HAp); applied with or without prior application of chlorhexidine (CHX), on remineralization of caries-affected and caries infected dentine types. Forty molars in eight patients having class I caries and no pulpal involvement were selected. In each selected molar, caries was removed except from the pulpal floor. Thereafter, molars were divided into two groups (n= 20) according to the type of carious dentine left on the pulpal floor; caries-affected or infected dentine. Each group was further subdivided into two subgroups (n=10) according to the material that was applied on carious dentine; n-HAp with or without 2% CHX. All cavities were finally sealed with glass ionomer restoration. Assessment of mineral density was performed at base line, one and three months using digitized radiography. The results showed increased mineral density in caries-affected dentine group; with no significant difference neither n-HAp was applied with or without CHX, nor was the application time increased more than one month. In caries-infected dentine group, however, mineral density was decreased. Such decrease was significantly evident at the third month; when n-HAp was applied without CHX. It was concluded that n-HAp could only promote remineralization of cariesaffected dentine. Additionally, although application of CHX prior to n-HAp showed no considerable influence on remineralization, yet, it could be recommended in case of caries-infected dentine as an antiseptic.

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“…CHX reduces in situ dentine erosion partially as solution and completely when used as gel [Kato et al, 2010a]. Interestingly, a concentration-dependent increase in demineralized dentine elastic modulus and surface mineral deposition was seen when CHXtreated dentine was incubated in simulated body fluid [Kim et al, 2011], indicating that enzyme inhibition may improve dentine collagen remineralization. CHX also inhibits release of the non-collagenous proteins decorin, biglycan and dentine sialoprotein from the demineralized dentine matrix by MMP-3 [Khaddam et al, 2014], which might contribute to the remineralization seen with CHX.…”

Section: Enzyme Inhibitionmentioning

confidence: 99%

Role of Host-Derived Proteinases in Dentine Caries and Erosion

2015

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Demineralization in dentinal caries and erosion exposes dentine organic matrix. This exposed matrix, containing type I collagen and non-collagenous proteins, is then degraded by host collagenolytic enzymes, matrix metalloproteinases (MMPs) and cysteine cathepsins. The knowledge of the identities and function of these enzymes in dentine has accumulated only within the last 15 years, but has already formed a field of research called ‘dentine degradomics'. This research has demonstrated the role of endogenous collagenolytic enzymes in caries and erosion development. In demineralized dentine, the enzymes degrade triple-helical collagen molecules, leading to the gradual loss of collagen matrix. Even before that, they can cleave off the terminal non-helical ends of collagen molecules called telopeptides, leading to the structural changes at the intramolecular gap areas, which may affect or even prevent intrafibrillar remineralization, which is considered essential in restoring the dentine's mechanical properties. They may also cause the loss of non-collagenous proteins that could serve as nucleation sites for remineralization. Here we review the findings demonstrating that inhibition of salivary or dentine endogenous MMPs and cysteine cathepsins may provide preventive means against the progression of caries or erosion. Furthermore, we also suggest the future directions for the new experimental preventive research to gain more knowledge of the enzymes and their function during and after dentine demineralization, and the pathways to find the clinically acceptable means to prevent the functional activity of these enzymes.

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The influence of chlorhexidine on the remineralization of demineralized dentine

Cited by 48 publications

References 45 publications

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Ex vivo detection and characterization of remineralized carious dentin, by nanoindentation and single point Raman spectroscopy, after amalgam restoration

Effect of Nanohydroxyapatite with and without Chlorhexidine on Remineralization of Carious Dentine at Different Intervals: An in vivo study

Role of Host-Derived Proteinases in Dentine Caries and Erosion

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