The influence of chemotactic factors on neutrophil adhesiveness

Abstract

The ability of several chemotactic factors to alter polymorphonuclear neutrophil (PMN) adhesiveness to nylon fibers was studied. Partly purified bacterial chemotactic factor, the isolated chemotactic fragment of human C5, and the chemotactic synthetic tripeptide, formyl-methionyl-leucyl-phenylalanine, transiently enhanced the nylon fiber adhesiveness of rabbit peritoneal PMSs. The capacity of these chemotactic factors to augment PMN adherence closely paralleled their ability to aggregate PMNs in suspension and to induce neutropenia when infused into rabbits. However, at least a portion of the adhreence-augmenting capacity of these agents was independent of their ability to induce PMN aggregation. Thus, chemotactic factors appear to transiently enhance PMN adhesiveness to a variety of surfaces. This hyper-adhesiveness may underlie the augmented nylon fiber adherence, aggregation, and neutropenia induced by these factors.

Neutropenia induced by systemic infusion of 5,12-dihydroxy-6,8,10,14-eicosatetraenoic acid: correlation with its in vitro effects upon neutrophils.

“…Thus, these data are fully compatible with the notion that compound III causes circulating PMN to sequester transiently in lung. Similar events occur with other PMN stimuli (8,11,27,30).…”

“…However, the in vitro endothelial studies were performed only after 0.5 h of PMN-endothelial cell incubation: the kinetics of this response appear quite different that those for the in vivo response (29). Furthermore, PMN harvested from C5a-induced neutropenic animals are hyperadherent to foreign surfaces (27,30 Neutropenia induced by compound III developed concomitantly with an influx of PMN into lung (Fig. 4).…”

Subcellular localization of the large subunit of Mo1 (Mo1 alpha; formerly gp 110), a surface glycoprotein associated with neutrophil adhesion.

“…Previous studies have shown that substances that provoke specific granule discharge also elicit an increase in the adhesiveness of neutrophils to surfaces (26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36). It is possible that the transfer of Mole to the plasma membrane is at least partially responsible for the increased adhesiveness characteristic of degranulated neutrophils.…”

Thromboxane A2 Mediates Augmented Polymorphonuclear Leukocyte Adhesiveness

“…Our studies demonstrate that arachidonic acid increases PMN adherence, an effect that can be blocked by cyclo-oxygenase and Tx synthetase inhibitors. Furthermore, the lack of effect of 8,11,14-eicosatrienoic acid, which is converted hy the platelet to PGH, and ultimately to monoenoic PG but not TxA, (27) (41,42). Enhanced adhesiveness to nylon in our system persisted to 60 min and, in the instance of arachidonic acid stimulation, increased at 120 min.…”