The influence of bubble size on chondrogenic differentiation of adipose-derived stem cells in gelatin microbubble scaffolds

Wu, Kuan Han; Mei, Chieh; Lin, Che; Yang, Kai Chiang; Yu, Jihong

doi:10.1039/c7tb02244a

Cited by 17 publications

(17 citation statements)

References 31 publications

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“…Similarly, other groups developed the microfluidic apparatus based on flow‐focusing technology for scaffold preparation (Wang et al, ). In addition to the differences in liquid flow rate, air pressure, gelatin and surfactant concentrations, the major improvement in our study is the collection device for the gelatin bubbles (Wu et al, ). We used the silicon wafer with a polydimethylsiloxane mold which is fabricated by soft lithography for bubble collection, and a scaffold with more compact structure can be prepared (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…A polydimethylsiloxane‐based (Sil‐More Industrial Ltd., Taipei, Taiwan) microfluidic device was used to fabricate the 3D gelatin bubble‐based scaffolds (Wu, Mei, Lin, Yang, & Yu, ). Each microfluidic channel houses an input for liquid component and gas, as well as an outflow for gelatin microbubbles (Figure ).…”

Section: Methodsmentioning

confidence: 99%

“…We used the silicon wafer with a polydimethylsiloxane mold which is fabricated by soft lithography for bubble collection, and a scaffold with more compact structure can be prepared (Figure 1). Our previous study also revealed that the mechanical property of this scaffold can be adjusted for different applications (Wu et al, 2018).…”

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confidence: 87%

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Three‐dimensional spherical gelatin bubble‐based scaffold improves the myotube formation of H9c2 myoblasts

Mei

Chao

Lin

et al. 2019

Biotech & Bioengineering

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Microenvironmental factors including physical and chemical cues can regulate stem cells as well as terminally differentiated cells to modulate their biological function and differentiation. However, one of the physical cues, the substrate's dimensionality, has not been studied extensively. In this study, the ﬂow‐focusing method with a microﬂuidic device was used to generate gelatin bubbles to fabricate highly ordered three‐dimensional (3D) scaffolds. Rat H9c2 myoblasts were seeded into the 3D gelatin bubble‐based scaffolds and compared to those grown on 2D gelatin‐coating substrates to demonstrate the influences of spatial cues on cell behaviors. Relative to cells on the 2D substrates, the H9c2 myoblasts were featured by a good survival and normal mitochondrial activity but slower cell proliferation within the 3D scaffolds. The cortical actin filaments of H9c2 cells were localized close to the cell membrane when cultured on the 2D substrates, while the F‐actins distributed uniformly and occupied most of the cell cytoplasm within the 3D scaffolds. H9c2 myoblasts fused as multinuclear myotubes within the 3D scaffolds without any induction but cells cultured on the 2D substrates had a relatively lower fusion index even differentiation medium was provided. Although there was no difference in actin α 1 and myosin heavy chain 1, H9c2 cells had a higher myogenin messenger RNA level in the 3D scaffolds than those of on the 2D substrates. This study reveals that the dimensionality influences differentiation and fusion of myoblasts.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Three‐dimensional spherical gelatin bubble‐based scaffold improves the myotube formation of H9c2 myoblasts

Mei

Chao

Lin

et al. 2019

Biotech & Bioengineering

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“…In the treatment of cartilage defects, the differentiation of stem cells into chondrocytes is an important cue [ 81 ]. Many physical and mechanical characteristics of the ECM, such as hypoxia, stiffness, topography, and pore size, are closely related to the interdifferentiation process of chondrocytes and stem cells [ 82 ].…”

Section: New Discoveries About Cancer and Other Diseases Based Onmentioning

confidence: 99%

“…Many physical and mechanical characteristics of the ECM, such as hypoxia, stiffness, topography, and pore size, are closely related to the interdifferentiation process of chondrocytes and stem cells [ 82 ]. Kuan-Han Wu et al [ 81 ] used gelatin as ECM in a microfabrication process to create a scaffold with different sizes of microbubbles and pores. They found that the size of microbubbles and pores can impact chondrogenesis, and a larger pore size is required for inducing mature chondrogenesis.…”

Section: New Discoveries About Cancer and Other Diseases Based Onmentioning

confidence: 99%

Microfabrication-Based Three-Dimensional (3-D) Extracellular Matrix Microenvironments for Cancer and Other Diseases

Song

Wang

Liu

et al. 2018

IJMS

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Exploring the complicated development of tumors and metastases needs a deep understanding of the physical and biological interactions between cancer cells and their surrounding microenvironments. One of the major challenges is the ability to mimic the complex 3-D tissue microenvironment that particularly influences cell proliferation, migration, invasion, and apoptosis in relation to the extracellular matrix (ECM). Traditional cell culture is unable to create 3-D cell scaffolds resembling tissue complexity and functions, and, in the past, many efforts were made to realize the goal of obtaining cell clusters in hydrogels. However, the available methods still lack a precise control of cell external microenvironments. Recently, the rapid development of microfabrication techniques, such as 3-D printing, microfluidics, and photochemistry, has offered great advantages in reconstructing 3-D controllable cancer cell microenvironments in vitro. Consequently, various biofunctionalized hydrogels have become the ideal candidates to help the researchers acquire some new insights into various diseases. Our review will discuss some important studies and the latest progress regarding the above approaches for the production of 3-D ECM structures for cancer and other diseases. Especially, we will focus on new discoveries regarding the impact of the ECM on different aspects of cancer metastasis, e.g., collective invasion, enhanced intravasation by stress and aligned collagen fibers, angiogenesis regulation, as well as on drug screening.

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Biofabrication of Gelatin Tissue Scaffolds with Uniform Pore Size via Microbubble Assembly

Bayram

Jiang

Gultekinoglu

et al. 2019

Macro Materials & Eng

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The control of pore size and uniform porosity remains as an important challenge in gelatin scaffolds. The precise control in building blocks of tissue scaffolds without any additional porogen is possible with costly equipment and techniques, though some pre‐requirements for polymeric material, such as photo‐polymerizability or sintering ability, may be needed prior to construction. Herein, a method for the fabrication of gelatin scaffolds with homogenous porosity using simple T‐junction microfluidics is described. The size of the microbubbles is precisely controlled with 5% deviation from the average. Porous gelatin scaffolds are obtained by building‐up the monodispersed microbubbles in dilute cross‐linker solutions. The effect of cross‐linker density on pore diameter is also investigated. After cross‐linking, pore size of the resultant five scaffold groups are precisely controlled as 135 ± 11, 193 ± 11, 216 ± 9, 231 ± 5, and 250 ± 12 µm. Porosity ratios above 65% are achieved in every sample group. According to the cell culture experiments, structures support high cell adhesion, viability, and migration through the porous network via interconnectivity. This study offers a practical and economical approach for the preparation of porous gelatin scaffolds with homogenous porosity which can be utilized in diverse tissue engineering applications.

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The influence of bubble size on chondrogenic differentiation of adipose-derived stem cells in gelatin microbubble scaffolds

Abstract: In human bodies, cartilage tissue lacks the ability to heal when it encounters trauma or lesions.

Cited by 17 publications

References 31 publications

Three‐dimensional spherical gelatin bubble‐based scaffold improves the myotube formation of H9c2 myoblasts

Three‐dimensional spherical gelatin bubble‐based scaffold improves the myotube formation of H9c2 myoblasts

Microfabrication-Based Three-Dimensional (3-D) Extracellular Matrix Microenvironments for Cancer and Other Diseases

Biofabrication of Gelatin Tissue Scaffolds with Uniform Pore Size via Microbubble Assembly

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