The Influence of Bt Maize Cultivation on Communities of Arbuscular Mycorrhizal Fungi Revealed by MiSeq Sequencing

Zeng, Huilan; Zhong, Wang; Tan, Fengxiao; Shu, Yinghua; Feng, Ying; Wang, Jianwu

doi:10.3389/fmicb.2018.03275

Cited by 19 publications

(10 citation statements)

References 62 publications

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“…In this study, the AMF colonization and the AMF-PLFA content of Bt and non- Bt maize under e CO 2 were generally higher than those under a CO 2 ; therefore, it is presumed that elevated CO 2 did have a positive facilitation on the colonization of F. caledonium on maize (Diaz et al, 1993 ; Drigo et al, 2013 ; Becklin et al, 2016 ). Besides, there were no differences in the AMF colonization and AMF-PLFA content between Bt maize and its parental line of non- Bt maize; the results showed that the presence of the cry1Ie protein in maize did not affect the colonization of the AMF community, and it is consistent with the research report of Cheeke et al; Cheeke et al ( 2014 ; 2015 ; Zeng et al, 2018 ).…”

Section: Discussionsupporting

confidence: 89%

AMF Inoculation Can Enhance Yield of Transgenic Bt Maize and Its Control Efficiency Against Mythimna separata Especially Under Elevated CO2

Wang

Gao

et al. 2021

Front. Plant Sci.

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The promotion and application of transgenic Bt crops provides an approach for the prevention and control of target lepidopteran pests and effectively relieves the environmental pressure caused by the massive usage of chemical pesticides in fields. However, studies have shown that Bt crops will face a new risk due to a decrease in exogenous toxin content under elevated carbon dioxide (CO2) concentration, thus negatively affecting the ecological sustainability of Bt crops. Arbuscular mycorrhizal fungi (AMF) are important beneficial microorganisms that can effectively improve the nutrient status of host plants and are expected to relieve the ecological risk of Bt crops under increasing CO2 due to global climate change. In this study, the Bt maize and its parental line of non-transgenic Bt maize were selected and inoculated with a species of AMF (Funneliformis caledonium, synonyms: Glomus caledonium), in order to study the secondary defensive chemicals and yield of maize, and to explore the effects of F. caledonium inoculation on the growth, development, and reproduction of the pest Mythimna separata fed on Bt maize and non-Bt maize under ambient carbon dioxide concentration (aCO2) and elevated carbon dioxide concentration (eCO2). The results showed that eCO2 increased the AM fungal colonization, maize yield, and foliar contents of jasmonic acid (JA) and salicylic acid (SA), but decreased foliar Bt toxin content and Bt gene expression in Bt maize leaves. F. caledonium inoculation increased maize yield, foliar JA, SA contents, Bt toxin contents, and Bt gene expression in Bt maize leaves, and positively improved the growth, development, reproduction, and food utilization of the M. separata fed on non-Bt maize. However, F. caledonium inoculation was unfavorable for the fitness of M. separata fed on Bt maize, and the effect was intensified when combined with eCO2. It is indicated that F. caledonium inoculation had adverse effects on the production of non-Bt maize due to the high potential risk of population occurrence of M. separata, while it was just the opposite for Bt maize. Therefore, this study confirms that the AMF can increase the yield and promote the expression levels of its endogenous (JA, SA) and exogenous (Bt toxin) secondary defense substances of Bt maize under eCO2, and finally can enhance the insect resistance capacity of Bt crops, which will help ensure the sustainable utilization and safety of Bt crops under climate change.

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Section: Discussionsupporting

confidence: 89%

AMF Inoculation Can Enhance Yield of Transgenic Bt Maize and Its Control Efficiency Against Mythimna separata Especially Under Elevated CO2

Wang

Gao

et al. 2021

Front. Plant Sci.

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“…The PCRs were conducted using the following program: 3 min of denaturation at 95°C, 30 cycles of 30 s at 95°C, 30 s for annealing at 55°C, and 45 s for elongation at 72°C, and a final extension at 72°C for 10 min. The procedure for the second round of PCRs was the same as that for the first round, except for the cycle number, which was 35 [61].…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Long-term effects of mixed planting on arbuscular mycorrhizal fungal communities in the roots and soils of Juglans mandshurica plantations

Yan

Yang

et al. 2020

BMC Microbiol

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Background Establishing mixed plantations is an effective way to improve soil fertility and increase forest productivity. Arbuscular mycorrhizal (AM) fungi are obligate symbiotic fungi that can promote mineral nutrient absorption and regulate intraspecific and interspecific competition in plants. However, the effects of mixed plantations on the community structure and abundance of AM fungi are still unclear. Illumina MiSeq sequencing was used to investigate the AM fungal community in the roots and soils of pure and mixed plantations (Juglans mandshurica × Larix gmelinii). The objective of this study is to compare the differential responses of the root and rhizosphere soil AM fungal communities of Juglans mandshurica to long-term mixed plantation management. Results Glomus and Paraglomus were the dominant genera in the root samples, accounting for more than 80% of the sequences. Compared with that in the pure plantation, the relative abundance of Glomus was higher in the mixed plantation. Glomus, Diversispora and Paraglomus accounted for more than 85% of the sequences in the soil samples. The relative abundances of Diversispora and an unidentified genus of Glomeromycetes were higher and lower in the pure plantation, respectively. The Root_P samples (the roots in the pure plantation) had the highest number of unique OTUs (operational taxonomic units), which belonged mainly to an unidentified genus of Glomeromycetes, Paraglomus, Glomus and Acaulospora. The number of unique OTUs detected in the soil was lower than that in the roots. In both the root and soil samples, the forest type did not have a significant effect on AM fungal diversity, but the Sobs value and the Shannon, Chao1 and Ace indices of AM fungi in the roots were significantly higher than those in the soil. Conclusions Mixed forest management had little effect on the AM fungal community of Juglans mandshurica roots and significantly changed the community composition of the soil AM fungi, but not the diversity.

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“…The PCR reactions were conducted using the following program: 3 min of denaturation at 95 °C, 30 cycles of 30 s at 95 °C, 30 s for annealing at 55 °C, and 45 s for elongation at 72 °C, and a nal extension at 72 °C for 10 min. The procedure of the second round PCR reaction was same as the rst round, except for the cycle number, which was 35 [60].…”

Section: Dna Extraction and Pcr Ampli Cationmentioning

confidence: 99%

Long-term Effects of Mixed Management on Arbuscular Mycorrhizal Fungal Community of Root and Soil in Juglans mandshurica Plantation

Li¹,

Zhang²,

Yang³

et al. 2020

Preprint

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Background: The establishment of mixed plantations is an effective way to improve soil fertility and increase forest productivity. Arbuscular mycorrhiza (AM) fungi are a kind of obligated symbiotic fungi, which can promote plants absorption of mineral nutrients and regulate intraspecific and interspecific competition. However, the effects of mixture on the community structure and abundance of AM fungi are still unclear. The Illumina MiSeq sequencing technique was used to determine the AM fungal community in the roots and soils of pure and mixed plantations (Juglans mandshurica × Larix gmelinii). The objective is to compare the response from root and rhizosphere soil AM fungal communities of Juglans mandshurica to long-term mixed plantation management. Results: Glomus and Paraglomus were the dominant genus in root samples, which accounted for more than 80% of the sequences. Compared with the pure plantation, the relative abundance of Glomus was higher in mixed plantation. Glomus, Diversispora and Paraglomus accounted for more than 85% of the sequences in soil samples. The relative abundance of Diversispora and Unclassified_c__Glomeromycetes were higher and lower in pure plantation, respectively. Samples of the Root_P (roots in pure plantation) had the highest number of unique OTUs (Operational Taxonomic Units), which were mainly composed of sequences belonging to unclassified_c__Glomeromycetes, Paraglomus, Glomus and Acaulospora. The amount of unique OTU detected in soil in pure and mixed plantation was relatively close. In the same types of sample (whether root or soil), the forest type did not have significant effect on AM fungal diversity, but the Sobs, Shannon, Chao1 and Ace indices of AM fungi in the roots were significantly higher than those in the soil. Conclusions: The mixed forest management had little effect on the AM fungal community in the root of Juglans mandshurica, but significantly changed the community composition of the soil AM fungi rather than diversity.

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The Influence of Bt Maize Cultivation on Communities of Arbuscular Mycorrhizal Fungi Revealed by MiSeq Sequencing

Cited by 19 publications

References 62 publications

AMF Inoculation Can Enhance Yield of Transgenic Bt Maize and Its Control Efficiency Against Mythimna separata Especially Under Elevated CO2

AMF Inoculation Can Enhance Yield of Transgenic Bt Maize and Its Control Efficiency Against Mythimna separata Especially Under Elevated CO2

Long-term effects of mixed planting on arbuscular mycorrhizal fungal communities in the roots and soils of Juglans mandshurica plantations

Long-term Effects of Mixed Management on Arbuscular Mycorrhizal Fungal Community of Root and Soil in Juglans mandshurica Plantation

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