The Influence of Biofield Energy Treated Vitamin D<sub>3</sub> on Bone Health and Its Health Consequences in MG-63 Cell-Line

Abstract

The aim of this study was to assess the effect of Consciousness Energy Healing-based vitamin D 3 and DMEM medium on bone health parameters such as alkaline phosphatase (ALP), collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The test items (vitamin D 3 and DMEM), were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Elizabeth Patric and those samples were denoted as the Biofield Energy Treated (BT) samples, while the other parts of each sample were referred as the untreated test items (UT). The MTT cell viability assay revealed that the test samples were found as safe in the tested concentrations. The experimental results showed that the level of ALP was remarkably increased by 88.62% and 70.48% in the BT-DMEM + UT-Test item (UT-TI) and BT-DMEM + BT-TI groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-TI group. Moreover, the collagen level was significantly increased by 252.59%, 50.55%, and 94.99% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 10 µg/mL compared to the UT-DMEM + UT-TI group. Further, the collagen level was significantly increased by 120.90% and 72.66% in the UT-DMEM + BT-TI and BT-DMEM + UT-TI groups, respectively at 50 µg/mL, while 34.99% in the UT-DMEM + BT-TI at 100 µg/mL compared to the untreated group. Apart from this, the percent of bone mineralization was distinctly enhanced by 127.13%, 225.12%, and 106.28% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 0.1 µg/mL compared to the untreated group. Moreover, the percentage of bone mineralization was significantly increased by 52.86%, 67.00%, and 23.62% in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 1 µg/mL compared to the untreated group. Additionally, the percentage of bone mineralization was significantly increased by 46.57% and 51.36% in the UT-DMEM + BT-TI and BT-DMEM + UT-TI groups, respectively at 10 µg/mL compared to the untreated group. Altogether, the Biofield Energy Treated vitamin D 3 was significantly improved the bone health parameters and it could be an alternative approach for nutraceutical supplement to combat vitamin D 3 deficiency and able to fight against various bone-related disorders including rickets, low bone density, osteomalacia, bone and joint pain, bone fractures, osteoporosis, osteoma, osteogenesis imperfecta, Paget's disease, deformed bones, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.

Evaluation of Anti-oxidation and Therapeutic Effect of Biofield Energy Healing Based Novel Test Formulation Using TNBS (Tri Nitro Benzene Sulfonic Acid) - Induced Ulcerative Colitis in Sprague Dawley Rats

“…Effect ® -Consciousness Energy Healing) have been studied and reported with significant outcomes in various scientific disciplines such as microbiology with altered antimicrobial sensitivity against pathogenic microbes [15][16][17], genetics [18,19], skin health [20,21], bone health [22][23][24] agricultural science [25,26], immunity [27,28], pharmaceuticals [29,30], and materials science [31,32]. In the present study, the authors evaluated the impact of the Biofield Energy (the…”

Evaluation of Disease Index, Colon Damage Score, and Microbial Analysis of Intestinal Flora after Treatment with the Novel Test Formulation in TNBS-Induced Colitis in Sprague Dawley Rats

“…Every living organism possess some kind of unique energy that can be harnessed and convey it into other living and non-living things by the process of Biofield Energy Healing by altered atomic/molecular weights through possible mediation of neutrinos [14]. Biofield Energy Healing Treatment/Blessing (the Trivedi Effect ® -Consciousness Energy Healing) have been studied and reported with significant outcomes in various scientific disciplines such as microbiology with altered antimicrobial sensitivity against pathogenic microbes [15][16][17], genetics [18,19], skin health [20,21], bone health [22][23][24] agricultural science [25,26], immunity [27,28], pharmaceuticals [29,30], and materials science [31,32].…”