The influence of bilateral superior cervical ganglionectomy, continuous light and continuous darkness on the diurnal rhythm of hydroxymethylglutaryl‐coenzyme a reductase in rat liver

Huber, J.; Latzin, Stefanie; Langguth, O.; Brauser, B.; Gabel, Virginie; Hamprecht, Bernd

doi:10.1016/0014-5793(73)80117-6

Cited by 13 publications

(6 citation statements)

References 14 publications

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“…Those that increase HMG-CoA reductase activity include insulin (White, 1972), thyroid hormone (Guder et al, 1968), adrenaline and noradrenaline (Edwards, 1973). Hormones which have been shown to decrease HMG-CoA reductase include glucagon (Huber et al, 1973) and the glucocorticoids (Dugan et al, 1974). The sex steroids, oestrogens, progestagens and androgens, can also suppress *Reprint requests to Dr J.…”

Section: Introductionmentioning

confidence: 99%

Indirect regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by oestradiol in the rabbit corpus luteum

McLean

Miller²

1988

Reproduction

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Rabbits were given 50 i.u. hCG, i.v., to initiate ovulation and pseudopregnancy (Day 0) and were treated, s.c., with or without a 1-cm Silastic oestradiol implant. Serum progesterone concentrations were measured at 4-day intervals and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase activity was estimated by the conversion of HMG to mevalonate in microsomes from corpora lutea removed on Days 4, 8, 12, 16 and 20 of pseudopregnancy (4 rabbits/day). Total HMG-CoA reductase activity was significantly (P less than 0.05) higher in control rabbits on Days 8 and 12 (5.29 +/- 0.63 and 5.5 +/- 0.28 nmol/min/mg protein, respectively) compared to oestradiol-treated rabbits (2.57 +/- 0.25 and 4.03 +/- 0.23 nmol/min/mg protein, respectively). On Days 16 and 20, total HMG-CoA reductase activity was not different in control and oestradiol-treated animals. There was no difference in the levels of the active fraction of HMG-CoA reductase, which represented less than 20% of the total enzyme activity, in control and oestradiol-treated rabbits (less than 780 pmol/min/mg protein, Day 12). These results indicate that oestradiol does not alter the active form, but can reduce the total activity of HMG-CoA reductase in the rabbit corpus luteum without a decline in serum progesterone. Therefore, neither total nor active forms of HMG-CoA reductase are directly related to progesterone secretion. This suggests that other sources of cholesterol may contribute to progesterone production in the rabbit.

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Section: Introductionmentioning

confidence: 99%

Indirect regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by oestradiol in the rabbit corpus luteum

McLean

Miller²

1988

Reproduction

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“…The microsomal fractions containing the hydroxymethylglutaryl-CoA reductase activity were prepared from liverl 14 ! and assayed for activity of the enzyme as described elsewheret 15 !. The food intake and the body weights of all rats were determined at least once a day.…”

Section: Methodsmentioning

confidence: 99%

“…Adrenalectomy affects neither the activity nor the halb 6 Stunden -auf die hohen Vergleichswerte scheinoperierter Tiere an. 3. Insulininjektion in hypophysektomierte Ratten erhöhte die niedrigen Reduktase-Aktivitäten nicht.…”

Section: Eine An Hypophysektomierte Ratten Verabreichte Einzeldosis Tunclassified

Influence of Hypophysectomy, Thyroid Hormone and Insulin on the Activity of 3-Hydroxy-3-methylglutaryl-Coenzyme A Reductase in Rat Liver

Huber¹,

Guder²,

Müller³

et al. 1974

Biological Chemistry

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1) Hypophysectomy abolishes the diurnal rhythm of hepatic hydroxymethylglutarylCoA reductase activity in rats.2) A single dose of triiodothyronine applied to hypophysectomized rats restored the activity of the enzyme to the high control levels of sham-operated animals within 48 hours, but failed to do so within 6 hours.3) Injection of insulin into hypophysectomized rats failed to increase the low reductase activities. 4) The interrelationship of thyroid hormone and insulin in the regulation of hepatic cholesterol synthesis is discussed.

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“…Behandlung des Fragments T2 mit Thermolysin (Th) bei 25 °C liefert die Bruchstücke T2Thi und T2Th2 [1] . Unter Einbeziehung der Aminosäuresequenz und der Sulfidbrückenzuordnung des Bruchstücks 2 1 2 [6] Zu der seit langem bekannten antibiotischen Wirksamkeit des Nisinst 10 ] und Subtilins [3] kommen nach neuesten Untersuchungen im Laboratorium der Autoren membranaktive Eigenschaften, die sich beispielsweise darin manifestieren, daß die Peptide und/oder einige ihrer Fragmente aus Lysosomen Enzyme freisetzen und daß sie imstande sind, einen Einfluß auf das entwicklungsphysiologische Geschehen im Uterus zu nehmen, indem sie dort -im Tierversuch mit Ratten und Kaninchen -fötale Resorption auszulösen vermögen. läßt sich mit Hilfe der folgenden analytischen Reaktionen bestimmen.…”

Section: Anzahlunclassified

Short Communication

1973

Hoppe-Seyler´s Zeitschrift für physiologische Chemie

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Mitochondriale H^Oz-Bildung an "Site //" Zusammenfassung: In Mitochondrien wird H2O2 gebildet. Die Bildungsrate von mitochondrialem Ü2O2 ist unabhängig vom Redoxzustand der Cytochrome 6562 und ci. Ein Block des Elektronentransportes durch Cytochrome-c-Extraktion induziert keine meßbare H2O2-Bildung. Antimycin reduziert unter diesen Bedingungen spezifisch Cytochrome 6566 und parallel dazu induziert es die Bildung von HfeOa. In Membranfragmenten von RinderherzmitochondrienIt was shown recently that mitochondria have to be regarded as a novel source of cellular H2O2 production d-4]t As detected either by the psroxidase-linked oxidation of the fluorescent dye scopoletint 1 · 4 ! or by the formation of enzyme-substrate complex by the cytochrome c peroxidase [2 -3] it was shown that tightly coupled mitochondria in state 4^ produce H2O2 when supplemented with succinate. The sensitivity of the rate of H2Ü2 formation towards inhibitors of respiration and uncouplers of oxidative phosphorylation identified the mitochondrial respiratory chain as the source of its formation. In our previous communication^ we discussed the possibility that the flavin moiety in the succinate dehydrogenase complex might be responsible for mitochondrial H2Ü2 generation. The obvious dependency of Ü2O2

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The influence of bilateral superior cervical ganglionectomy, continuous light and continuous darkness on the diurnal rhythm of hydroxymethylglutaryl‐coenzyme a reductase in rat liver

Cited by 13 publications

References 14 publications

Indirect regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by oestradiol in the rabbit corpus luteum

Indirect regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by oestradiol in the rabbit corpus luteum

Influence of Hypophysectomy, Thyroid Hormone and Insulin on the Activity of 3-Hydroxy-3-methylglutaryl-Coenzyme A Reductase in Rat Liver

Short Communication

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