The Influence of Behavioral, Social, and Environmental Factors on Reproducibility and Replicability in Aquatic Animal Models

Lieggi, Christine; Kalueff, Allan V.; Lawrence, Christian; Collymore, Chereen

doi:10.1093/ilar/ilz019

Cited by 21 publications

(9 citation statements)

References 201 publications

(286 reference statements)

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“…2, Tables 2, 3). However, some other reports failed to evoke overt anxiety 69 , raising questions of whether CUS protocol was indeed properly applied in such studies (e.g., see 70,71 for a recent discussion of challenges with data reliability and replicability in zebrafish behavioral models). Thus, further research is needed, for example, to compare zebrafish that received CUS protocols that would differ in the numbers of stressors, their severity and/or duration.…”

Section: Discussionmentioning

confidence: 99%

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Demin

Lakstygal

Krotova

et al. 2020

Sci Rep

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Stress-related neuropsychiatric disorders are widespread, debilitating and often treatment-resistant illnesses that represent an urgent unmet biomedical problem. Animal models of these disorders are widely used to study stress pathogenesis. A more recent and historically less utilized model organism, the zebrafish (Danio rerio), is a valuable tool in stress neuroscience research. Utilizing the 5-week chronic unpredictable stress (CUS) model, here we examined brain transcriptomic profiles and complex dynamic behavioral stress responses, as well as neurochemical alterations in adult zebrafish and their correction by chronic antidepressant, fluoxetine, treatment. Overall, CUS induced complex neurochemical and behavioral alterations in zebrafish, including stable anxiety-like behaviors and serotonin metabolism deficits. Chronic fluoxetine (0.1 mg/L for 11 days) rescued most of the observed behavioral and neurochemical responses. Finally, whole-genome brain transcriptomic analyses revealed altered expression of various CNS genes (partially rescued by chronic fluoxetine), including inflammation-, ubiquitin- and arrestin-related genes. Collectively, this supports zebrafish as a valuable translational tool to study stress-related pathogenesis, whose anxiety and serotonergic deficits parallel rodent and clinical studies, and genomic analyses implicate neuroinflammation, structural neuronal remodeling and arrestin/ubiquitin pathways in both stress pathogenesis and its potential therapy.

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Section: Discussionmentioning

confidence: 99%

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Demin

Lakstygal

Krotova

et al. 2020

Sci Rep

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“…Good health is an essential, but not unique, component of good animal welfare. To maintain the fish colonies in good health and warrant reproductive success and unambiguous experimental data, sound husbandry standards and emphasis on stress reduction must be in place [ 30 , 31 ], as well as biosecurity measures to limit pathogen exposure. Important arenas for biosecurity precaution are health monitoring, import processes, mixing of species, and protection of operators.…”

Section: Discussionmentioning

confidence: 99%

A FELASA Working Group Survey on Fish Species Used for Research, Methods of Euthanasia, Health Monitoring, and Biosecurity in Europe, North America, and Oceania

Mocho

Krogh²

2022

Biology

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An international survey was conducted regarding species used for research, methods of euthanasia, health monitoring, and biosecurity in fish laboratories. A total of 145 facilities from 23 countries contributed. Collectively, over 80 different species (or groups of species) were reported to be used for research, of which zebrafish (Danio rerio) was the most common by far. About half of the participating laboratories used multiple species. Anesthetic overdose was the preferred method for euthanasia for adult, fry (capable of independent feeding), and larval (not capable of independent feeding) fish. For all developmental stages, the most popular anesthetic compound was tricaine (MS-222), a substance associated with distress and aversion in several species. Moreover, around half of the respondents did not perform a completion method to ensure death. One-quarter of the responding facilities did not have a health monitoring system in place. While most respondents had some form of quarantine process for imported fish, only a small fraction reported quarantine routines that ensure reliable biological barriers. Furthermore, less than one in five screened fish for pathogens while in quarantine. In sum, there was little consensus amongst facilities in how to perform biosecurity measures. Regarding euthanasia, health monitoring, and biosecurity processes, there is a need for updated and universal guidelines and for many laboratories to adjust their practices.

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“…Advances in the science of zebrafish husbandry and management and more detailed reporting of maintenance conditions of experimental fish are needed to address this issue (reviewed by Lieggi et al . 2020 ). Most guidelines are concerned primarily with maximising production and minimising costs and pay less attention to how aspects of the laboratory environment affect welfare or the science that they support.…”

Section: Introductionmentioning

confidence: 99%

Improving zebrafish laboratory welfare and scientific research through understanding their natural history

2022

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Globally, millions of zebrafish (Danio rerio) are used for scientific laboratory experiments for which researchers have a duty of care, with legal obligations to consider their welfare. Considering the growing use of the zebrafish as a vertebrate model for addressing a diverse range of scientific questions, optimising their laboratory conditions is of major importance for both welfare and improving scientific research. However, most guidelines for the care and breeding of zebrafish for research are concerned primarily with maximising production and minimising costs and pay little attention to the effects on welfare of the environments in which the fish are maintained, or how those conditions affect their scientific research. Here we review the physical and social conditions in which laboratory zebrafish are kept, identifying and drawing attention to factors likely to affect their welfare and experimental science. We also identify a fundamental lack knowledge of how zebrafish interact with many biotic and abiotic features in their natural environment to support ways to optimise zebrafish health and well-being in the laboratory, and in turn the quality of scientific data produced. We advocate that the conditions under which zebrafish are maintained need to become a more integral part of research and that we understand more fully how they influence experimental outcome and in turn interpretations of the data generated.

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The Influence of Behavioral, Social, and Environmental Factors on Reproducibility and Replicability in Aquatic Animal Models

Cited by 21 publications

References 201 publications

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

Understanding complex dynamics of behavioral, neurochemical and transcriptomic changes induced by prolonged chronic unpredictable stress in zebrafish

A FELASA Working Group Survey on Fish Species Used for Research, Methods of Euthanasia, Health Monitoring, and Biosecurity in Europe, North America, and Oceania

Improving zebrafish laboratory welfare and scientific research through understanding their natural history

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