The influence of association between aging and reduced protein intake on some immunomodulatory aspects of bone marrow mesenchymal stem cells: an experimental study

Silva, Renaira Oliveira da; Hastreiter, Araceli Aparecida; Vivian, Gabriela Kodja; Dias, Carolina; Santos, Andressa Cristina Antunes; Makiyama, Edson Naoto; Borelli, Primavera; Fock, Ricardo Ambrósio

doi:10.1007/s00394-022-02893-0

Cited by 4 publications

(1 citation statement)

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“…BMSCs were first identified by Friedenstein, Chailakhyan & Gerasimov (1987) as fibroblast colonies formed by bone marrow explants. Former studies show that BMSCs can promote the homing of transplanted bone marrow cells through specific chemokines and their receptors, such as CXCL1 ( Wang et al, 2021 ), CXCL12, CCL21 and CCL27, and augment hematopoiesis by secreting multiple cytokines such as IL 6, IL 10, TGF- β ( Jin et al, 2018 ; Heirani-Tabasi et al, 2018 ; Hocking, 2015 ; Mohammadali, Abroun & Atashi, 2018 ; Kim et al, 2015 ; Köse et al, 2018 ; da Silva et al, 2022 ; Guo et al, 2018a ; Hastreiter et al, 2021 ; Sun et al, 2021 ). Moreover, the BMSCs have been shown to mitigate severe, steroid-refractory aGVHD in the liver, skin and lungs, although the exact mechanisms are unclear ( Song et al, 2015a ; Lee et al, 2015 ; Auletta et al, 2015 ; Bonig et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Influence of SPIO labelling on the function of BMSCs in chemokine receptors expression and chemotaxis

Liu

Huang

Wang

et al. 2023

PeerJ

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Bone marrow-derived mesenchymal stem cells (BMSCs) are increasingly being used in bone marrow transplantation (BMT) to enable homing of the allogeneic hematopoietic stem cells and suppress acute graft versus host disease (aGVHD). The aim of this study was to optimize the labelling of BMSCs with superparamagnetic iron oxide particles (SPIOs), and evaluate the impact of the SPIOs on the biological characteristics, gene expression profile and chemotaxis function of the BMSCs. The viability and proliferation rates of the SPIO-labeled BMSCs were analyzed by trypan blue staining and CCK-8 assay respectively, and the chemotaxis function was evaluated by the transwell assay. The expression levels of chemokine receptors were measured by RT-PCR and flow cytometry. The SPIOs had no effect on the viability of the BMSCs regardless of the labelling concentration and culture duration. The labelling rate of the cells was higher when cultured for 48 h with the SPIOs. Furthermore, cells labeled with 25 µg/ml SPIOs for 48 h had the highest proliferation rates, along with increased expression of chemokine receptor genes and proteins. However, there was no significant difference between the chemotaxis function of the labeled and unlabeled BMSCs. To summarize, labelling BMSCs with 25 µg/ml SPIOs for 48h did not affect their biological characteristics and chemotaxis function, which can be of significance for in vivo applications.

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