The Influence of Alloxan and Dietary Restriction on Rat Liver Desoxyribonucleic Acid (Dna)

Conrad, Eugene A.; Bass, Allan D.

doi:10.1177/5.2.182

Cited by 11 publications

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“…Conrad and Bass (8) in studying liver, reported that rats subjected to a fast for a period of 48 hours showed an increase in nuclei of higher ploidy, a decrease in the total number of nuclei, and an increase in the amount of D N A per average nucleus. The results of the present experiment are quite different and therefore make anorexia an unlikely explanation for the observations reported.…”

Section: Resultsmentioning

confidence: 99%

Cytophotometric Study of Deoxyribonucleic Acid in Cortisone-Treated Rat Hepatocytes

Lowe

Box

Venkataraman

et al. 1959

The Journal of Cell Biology

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Rats were treated by intramuscular injection with cortisone acetate, 25 reg./day for 5 days. Small pieces of liver obtained from treated and normal animals were squashed on a microscope slide so as to obtain many areas only a single cell in thickness. After Feulgen staining to demonstrate DNA, optical density was measured using a projection technique.In both the normal and treated animals the nuclei were easily segregated in three ploidy classes, diploid, tetraploid, and octaploid, depending upon Feulgen intensity. In all three classes, the absorbence of nuclei from cortisone-treated animals was approximately 20 per cent lower than the normal. These data were interpreted to indicate that a change in DNA content had been induced by cortisone administration. These findings are comparable to data obtained from similar animals using chemical methods for the determination of DNA. Material and Methods Animals:Male rats of the Wistar strain 1, weighing from 100 to 120 grams, were fed ad libitum on a lab-blox R dietZ All animals were fasted for 24 hours before the removal of the livers and, to avoid alterations due to diurnal variation in metabolism, were killed between 9:00 and 10:00 A.M. One group of rats received cortisone acetate 3 by intramuscular injection, 25 reg./day for 5 days (cortisone-treated animals). Control animals received no injections (normal animals).In order to avoid variations in the diameter and thickness of nuclei in a tissue section, squash smears of small pieces of liver from both groups of animals were prepared. These were fixed immediately in Carnoy's (2) for 10 minutes and then stained by the Feulgen technique (2, 3). This technique gave thin smears with uniformly dispersed whole nuclei imbedded in whole or broken cell cytoplasm. Only areas one cell thick were studied.

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Section: Resultsmentioning

confidence: 99%

Cytophotometric Study of Deoxyribonucleic Acid in Cortisone-Treated Rat Hepatocytes

Lowe

Box

Venkataraman

et al. 1959

The Journal of Cell Biology

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show abstract

“…The effects of acute fasting on nuclear ploidy were also studied (Conrad and Bass 1957). By chemical and microspectrophotometric techniques these authors observed an increase in DNA per nucleus as well as a slight increase in the frequency of tetraploid (4n) and octaploid (8n) nuclei.…”

Section: Patterns In Protein Deficiencymentioning

confidence: 99%

Ploidy and biochemical characteristics of liver cell nuclei in rats fed different amounts of dietary protein

Moore

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Die reparative Parenchymregeneration am Beispiel der Leber nach Teilhepatektomie

Grundmann

Seidel

1969

Handbuch Der Allgemeinen Pathologie

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The Influence of Alloxan and Dietary Restriction on Rat Liver Desoxyribonucleic Acid (Dna)

Abstract: I (iFidelbilt (n iversily School of .hJe(!iCifle, .\ashville, Ten zessee Received for i)umblittot it)ii SeI)teunnbcn 28, 1956 * This work wtos siul)pont cdl b gnmuuuts fromnn Eli Lilly :unnul Co., mount! by Gntomut No.

Cited by 11 publications

References 0 publications

Cytophotometric Study of Deoxyribonucleic Acid in Cortisone-Treated Rat Hepatocytes

Cytophotometric Study of Deoxyribonucleic Acid in Cortisone-Treated Rat Hepatocytes

Ploidy and biochemical characteristics of liver cell nuclei in rats fed different amounts of dietary protein

Die reparative Parenchymregeneration am Beispiel der Leber nach Teilhepatektomie

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