The Influence of Allogeneic Cells on the Human T and B Cell Repertoire

Rood, Jon J. van; Claas, Frans H.J.

doi:10.1126/science.1972596

Cited by 106 publications

(34 citation statements)

References 48 publications

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“…31 This observation was apparently in keeping with data published by some authors on a blunted immune response to maternal cells or soluble HLA antigens, 30,33,34 but in contrast with results reported by others, 32,35 who did not show any difference in the CTLp frequency towards NIMA and NIPA. Results of the present study indicate that absence of response in 'bulk culture' does not seem to be due to deletion of NIMA-reactive cytotoxic cells, but it can rather be attributed to the action of regulatory populations, displaying their effect in 'bulk culture' and unable to carry out their function in a limiting dilution condition.…”

Section: Discussioncontrasting

confidence: 52%

“…29 However, these favourable clinical effects of NIMA have not found a biological explanation, since conflicting results on development of NIMA-specific CTL have been published. [30][31][32][33][34][35] We previously observed that CBMC from about 50% of neonates are unable to develop CTL activity when challenged in vitro with allogeneic cells in 'bulk culture' conditions. In the great majority of remaining individuals who were able to mount a cytotoxic response against paternal cells, CBMC were unable to kill cells of maternal origin.…”

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confidence: 99%

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Characterisation of CTL directed towards non-inherited maternal alloantigens in human cord blood

Moretta

Locatelli

Mingrat

et al. 1999

Bone Marrow Transplant

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Summary:Allogeneic cord blood transplantation (CBT), especially from unrelated donors, is being increasingly used for treating paediatric patients with both malignant and nonmalignant disorders. Recent clinical and experimental evidence suggests that human cord blood mononuclear cells (CBMC) may acquire in utero a state of tolerance towards non-inherited maternal antigens (NIMA). In order to better define this phenomenon, we measured, by means of a limiting dilution assay (LDA), the frequency of NIMA-specific CTL precursors ( Over the past decade, allogeneic cord blood transplantation (CBT) from both related and unrelated donors has been used increasingly for the treatment of paediatric patients with both malignant and non-malignant disorders. 1-4 Previously published results demonstrate that, at least when the donor is a compatible relative, CBT recipients experience a

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Section: Discussioncontrasting

confidence: 52%

mentioning

confidence: 99%

Characterisation of CTL directed towards non-inherited maternal alloantigens in human cord blood

Moretta

Locatelli

Mingrat

et al. 1999

Bone Marrow Transplant

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“…Donor-specific transfusions (DST) 3 induce both experimental and clinical donor-specific allograft tolerance (15,18,19,(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34).…”

Section: Development Of Infectious Tolerance After Donor-specificmentioning

confidence: 99%

Development of Infectious Tolerance After Donor-Specific Transfusion and Rat Heart Transplantation

Kataoka

Margenthaler

et al. 2003

The Journal of Immunology

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Regulatory cells developed after donor-specific transfusion (DST)-induced acceptance of a LEW heart transplanted into a DA rat. Both DST and the cardiac transplant were necessary to generate the regulatory cells. This donor-specific tolerance can then be transferred into a new DA recipient by adoptive transfer of lymphocytes from the DST-treated long term survivor (LTS) in a dose-dependent manner. The effectiveness of tolerance did not diminish over five generations of adoptive transfer, thus supporting its infectious nature. Although both spleen and lymph node cells were equally effective, graft-infiltrating lymphocytes were more potent. A high level of indirect CTL activity and MLC proliferation were observed in lymphocytes from LTS. In vivo tracking of adoptively transferred CFSE-labeled splenocytes from LTS showed equivalent FACS proliferation and a higher percentage of graft-infiltrating lymphocytes 7 days after heart transplantation, compared with adoptively transferred naive splenocytes. Adoptive transfer of CD8+-depleted LTS splenocytes resulted in 100% subsequent LEW allograft acceptance; whereas CD4+ depletion decreased acceptance to 40%, and depletion of both CD4 and CD8 resulted in 0% acceptance. When positively selected CD4+ or CD8+ cells were adoptively transferred, 100% or 62.5% of LEW cardiac allografts survived, respectively. In conclusion, DST alone promotes a donor-specific infectious tolerance of a heart graft that can be adoptively transferred to subsequent naive allograft recipients despite the undiminished in vitro immunological response to donor Ag. Although both CD4+ and CD8+ populations are responsible for the regulatory mechanism in DST-induced tolerance, the CD4+ population appears to dominate.

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“…Finally, in contrast to other experiences we were able to achieve excellent cell recovery using a Ficoll though to be due to the development of tolerance in utero to the non-inherited maternal allele. 24,25 In this case the gender separation technique resulting in prompt engraftment after infusion of cryopreserved cells. We therefore conclude that equity of the patient and donor and the low parity of the mother also decreased the likelihood of GVHD occurring.…”

Section: Parameter Resultsmentioning

confidence: 99%

Haploidentical related umbilical cord blood stem cell transplant in a child with acute non-lymphocytic leukemia

Katzenstein

Morgan

Olsewski

et al. 1997

Bone Marrow Transplant

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Summary:vious data had suggested that umbilical cord blood would serve as an excellent source of hematopoietic stem cells. 3 As the use of UCBSC transplants continues to develop Umbilical cord blood stem cells (UCBSC) were used to reconstitute hematopoiesis following myeloablative thermany questions still remain unanswered about cord blood as a source of stem cells. Umbilical cord blood has many apy in a 13-month-old infant with acute nonlymphocytic leukemia (ANLL):FAB-M5 who had failed to sustain a potential advantages over other stem cell sources, including a large, accessible donor population, ease and safety of colchemotherapeutic remission. The patient's mother was 18 weeks pregnant with her second child at the time lection, decreased incidence of infectious contamination and a decreased risk of GVHD. 4 of diagnosis. Amniocentesis revealed that the fetus was HLA-haploidentical with the patient at the paternallyThe morbidity and mortality of allogeneic BMT is highly dependent upon the HLA compatibility of the donor and inherited allele. The umbilical cord blood was harvested and processed by Ficoll centrifugation with 100% recovthe recipient as it influences the occurrence of GVHD. 5 Increasing the HLA disparity escalates the risk of ery of 5 ؋ 10 7 mononuclear cells/kg and then cryopreserved. Two weeks after collection the cells were thawed developing acute GVHD. The majority of the UCBSCtransplanted patients reported to date have been reconstiand then infused into the patient following conditioning with total body irradiation, cyclophosphamide, and etotuted from HLA-identical donors with minimal information on patients transplanted from donors with a three antigen poside. Graft-versus-host-disease (GVHD) prophylaxis consisted of cyclosporine and methotrexate. The patient mismatch. Previous reports have cited a lower incidence of both the occurrence and severity of GVHD in UCBSC experienced clinical grade I GVHD consisting of skin involvement only that resolved within 2 weeks following transplants. 1,[6][7][8][9] If this is indeed the case then it would suggest that umbilical cord blood stem cells with a greater the addition of corticosteroids. Engraftment was achieved with an absolute neutrophil count (ANC) above HLA-mismatch could be used with acceptable morbidity. There are a number of methods that have been used in 0.5 ؋ 10 9 /l on day 16, a platelet count above 50 ؋ 10 9 /1 on day 56, platelet transfusion independence on day 32 the collection and preparation of UCBSC. 1,[10][11][12][13][14] The ideal method for collection, processing, manipulation and storage and red blood cell transfusion independence after day 44. Three months following transplantation restriction is still to be determined. Most sources have warned that the manipulation of cord blood results in a significant fragment length polymorphisms (RFLP) revealed no discernible difference between the donor and the recipidepletion of stem cells and should be not be attempted. 2,3,5,10,11 The following case illustrates the sucent. The patien...

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The Influence of Allogeneic Cells on the Human T and B Cell Repertoire

Cited by 106 publications

References 48 publications

Characterisation of CTL directed towards non-inherited maternal alloantigens in human cord blood

Characterisation of CTL directed towards non-inherited maternal alloantigens in human cord blood

Development of Infectious Tolerance After Donor-Specific Transfusion and Rat Heart Transplantation

Haploidentical related umbilical cord blood stem cell transplant in a child with acute non-lymphocytic leukemia

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