The Influence of Adipose-Derived Stem Cells Induced into Endothelial Cells on Ectopic Vasculogenesis and Osteogenesis

Najdanović, Jelena G.; Cvetković, Vladimir J.; Stojanović, Sanja; Vukelić-Nikolić, Marija; Stanisavljević, Milica N.; Živković, Jelena; Najman, Stevo

doi:10.1007/s12195-015-0403-x

Cited by 18 publications

(13 citation statements)

References 61 publications

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“…ADSCs can be isolated from abundant and easily accessible adipose tissue in large quantities with a minimal invasive procedure either from liposuction aspirates or adipose tissue biopsies [2] and have properties that make them good candidates for regenerative medicine applications [3]. The use of ADSCs in tissue engineering and regenerative medicine is very promising due to their self-renewal potential, proliferation capacity and great potential to differentiate into numerous cell types particularly adipocytes, osteoblasts, chondrocytes and endothelial cells [4,5,6,7]. It is reported that ADSCs can be successfully differentiated into the cells of all three germ lines which means that they can be considered as pluripotent and makes them a good candidate for wide range applications in the field of biomedical sciences [4].…”

Section: Introductionmentioning

confidence: 99%

Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature

Stojanović

Najman

Korać

2018

Cells

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Lipomas are benign adipose tissue tumors of unknown etiology, which can vary in size, number, body localization and cell populations within the tissue. Lipoma-derived stem cells (LDSCs) are proposed as a potential tool in regenerative medicine and tissue engineering due to their similar characteristics with adipose-derived stem cells (ADSCs) reported so far. Our study is among the first giving detailed insights into the molecular signature and differences in the differentiation capacity of LDSCs in vitro compared to ADSCs. Mesenchymal stem cell phenotype was analyzed by gene expression and flow cytometric analysis of stem cell markers. Adipogenesis and osteogenesis were analyzed by microscopic analysis, cytochemical and immunocytochemical staining, gene and protein expression analyses. We showed that both LDSCs and ADSCs were mesenchymal stem cells with similar phenotype and stemness state but different molecular basis for potential differentiation. Adipogenesis-related genes expression pattern and presence of more mature adipocytes in ADSCs than in LDSCs after 21 days of adipogenic differentiation, indicated that differentiation capacity of LDSCs was significantly lower compared to ADSCs. Analysis of osteogenesis-related markers after 16 days of osteogenic differentiation revealed that both types of cells had characteristic osteoblast-like phenotype, but were at different stages of osteogenesis. Differences observed between LDSCs and ADSCs are probably due to the distinct molecular signature and their commitment in the tissue that governs their different capacity and fate during adipogenic and osteogenic induction in vitro despite their similar mesenchymal phenotype.

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Section: Introductionmentioning

confidence: 99%

Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature

Stojanović

Najman

Korać

2018

Cells

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“…Particularly, materials based on hydroxyapatite and β-tricalcium phosphate are suitable for BTE [42]. In addition, materials based on bone mineral matrix are frequently used [43][44][45][46]. Also, regulatory factors are not less important components in BTE, and at the first place, they should induce and support osteogenic differentiation, adhesion, and proliferation of implanted cells.…”

Section: Bone Substitutes and Regulatory Factorsmentioning

confidence: 99%

“…This might be the key for enhancing osteogenesis guided by freshly isolated and untreated ADSCs within SVF. Despite disadvantages of ectopic models, there are many literature data where ectopic subcutaneous implantations were conducted [43,44,71,75,76] because they allow examining the real potential of the implanted cells [65] alone and without the impact of the factors that are normally present in bone tissue. Therefore, selection of adequate animal model should depend on experimental goal, and finally it is important for interpretation and extrapolation of obtained results [1].…”

Section: One-step Versus Multistep Approachesmentioning

confidence: 99%

Application of Adipose-Derived Stem Cells in Treatment of Bone Tissue Defects

Najman¹,

Najdanović²,

Cvetković³

2021

Clinical Implementation of Bone Regeneration and Maintenance

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Despite excellent self-regeneration capacity of bone tissue, there are some large bone defects that cannot be healed spontaneously. Numerous literature data in the field of cell-based bone tissue engineering showed that adipose-derived stem cells (ADSCs) after isolation could be subsequently applied in a one-step approach for treatment of bone defect, without previous in vitro expansion and osteoinduction. However, standard approaches usually involve in vitro expansion and osteoinduction of ADSCs as an additional preparation step before its final application. Bioreactors are also used for the preparation of ADSC-based graft prior application. The commonly used approaches are reviewed, and their outcomes, advantages, disadvantages, as well as their potential for successful application in the treatment of bone defects are discussed. Difficulty in spontaneous healing of bone defects is very often due to poor vascularization. To overcome this problem, numerous methods in bone tissue engineering (BTE) were developed. We focused on freshly isolated stromal vascular fraction (SVF) cells and ADSCs in vitro induced into endothelial cells (ECs) as cells with vasculogenic capacity for the further application in bone defect treatment. We have reviewed orthotopic and ectopic models in BTE that include the application of SVFs or ADSCs in vitro induced into ECs, with special reference to co-cultivation.

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“…People with severe phenotypes most often experience frontal bossing, depression of the bridge of the nose, mandible and maxilla enlargement with the upper teeth exposure, bone pain, osteopenia and osteochondrosis. If spinal impairment occurs during the childhood, linear growth is delayed, resulting in the discordance in the length of upper and lower limbs [63,64,87]. The progressive enlargement of the abdomen is due to the hepatosplenomegaly, whereas the masses of extramedullar hematopoietic tissue may also be found in the chest or spinal column [63,64].…”

Section: Clinical Findingsmentioning

confidence: 99%

Adaptation to Mediterranea

Milenković¹,

Stojanović²,

Najdanović³

2021

Genetic Variation

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The Mediterranean region encompasses countries that surround Mediterranean Sea. Due to its position at the intersection of Eurasia and Africa it has often been a route of human migrations during history, which contributed to its high biodiversity. People living in this area had been exposed to the episodes of natural selection that led to the establishment of specific genetic variations, for which is thought to carry a certain adaptation. Some recent studies have shown that genetic adaptations are probably related to the immune defense against infectious pathogens. One of the most recognizable disease of the region is familial Mediterranean fever (FMF), a prototype of a monogenic autoinflammatory disease. FMF is predisposed by the mutations in the Mediterranean fever (MEFV) gene that encodes inflammasome regulatory protein - pyrin. Specific variations of several other genes have been proposed to confer a protection against Plasmodium malariae parasite. Some of these are hemoglobin S (HbS), thalassemia, glucose-6-phosphate dehydrogenase deficiency, ovalocytosis, and mutation in the Duffy antigen (FY). In this chapter we will summarize important genetics and pathogenesis features of diseases commonly encountered in the Mediterranean region with a short discussion of potential adaptations that they may carry.

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The Influence of Adipose-Derived Stem Cells Induced into Endothelial Cells on Ectopic Vasculogenesis and Osteogenesis

Cited by 18 publications

References 61 publications

Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature

Stem Cells Derived from Lipoma and Adipose Tissue—Similar Mesenchymal Phenotype but Different Differentiation Capacity Governed by Distinct Molecular Signature

Application of Adipose-Derived Stem Cells in Treatment of Bone Tissue Defects

Adaptation to Mediterranea

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