The influence of actin depolymerization induced by Cytochalasin D and mechanical stretch on interleukin-8 expression and JNK phosphorylation levels in human retinal pigment epithelial cells

Gao, Meng; Wu, Shen; Ji, Jing; Zhang, Jing-xue; Liu, Qian; Yue, Yankun; Liu, Lu; Liu, Xinxin; Liu, Wu

doi:10.1186/s12886-017-0437-z

Cited by 16 publications

(12 citation statements)

References 17 publications

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“…3q, s, t) was consistent with previous reports of IL-8 and VEGF induction in human RPE cells after RPE injury [29,30]. It has previously been shown that actin polymerization activation induces IL-8 expression and different VEGF isoforms [31,32] . It is therefore possible that disrupting the actin cytoskeleton activates actin polymerization, leading to the increased expression of IL-8 and different VEGF isoforms.…”

Section: Discussionsupporting

confidence: 92%

Acute mechanical stress in primary porcine RPE cells induces angiogenic factor expression and in vitro angiogenesis

et al. 2020

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Background: Choroidal neovascularization (CNV) is a major cause of blindness in patients with age-related macular degeneration. CNV is characterized by new blood vessel growth and subretinal fluid accumulation, which results in mechanical pressure on retinal pigment epithelial (RPE) cells. The overexpression of RPE-derived angiogenic factors plays an important role in inducing CNV. In this work, we investigated the effect of mechanical stress on the expression of angiogenic factors in porcine RPE cells and determined the impact of conditioned medium on in-vitro angiogenesis. Results: The goal of this study was to determine whether low levels of acute mechanical stress during early CNV can induce the expression of angiogenic factors in RPE cells and accelerate angiogenesis. Using a novel device, acute mechanical stress was applied to primary porcine RPE cells and the resulting changes in the expression of major angiogenic factors, VEGF, ANG2, HIF-1α, IL6, IL8 and TNF-α, were examined using immunocytochemistry, qRT-PCR, and ELISA. An in vitro tube formation assay was used to determine the effect of secreted angiogenic proteins due to mechanical stress on endothelial tube formation by human umbilical vein endothelial cells (HUVECs). Our results showed an increase in the expression of VEGF, ANG2, IL-6 and IL-8 in response to mechanical stress, resulting in increased in vitro angiogenesis. Abnormal epithelial-mesenchymal transition (EMT) in RPE cells is also associated with CNV and further retinal degeneration. Our qRT-PCR results verified an increase in the expression of EMT genes, CDH2, VIM and FN1, in RPE cells. Conclusions: In conclusion, we showed that acute mechanical stress induces the expression of major angiogenic and EMT factors and promotes in vitro angiogenesis, suggesting that mechanical stress plays a role in promoting aberrant angiogenesis in AMD.

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Section: Discussionsupporting

confidence: 92%

Acute mechanical stress in primary porcine RPE cells induces angiogenic factor expression and in vitro angiogenesis

et al. 2020

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show abstract

“…3q, s, t) was consistent with previous reports of IL -8 and VEGF induction in human RPE cells after RPE injury [30,31]. It has previously been shown that actin polymerization activation induces IL-8 expression and different VEGF isoforms [32,33] . It is therefore possible that disrupting the actin cytoskeleton activates actin polymerization, leading to the increased expression of IL-8 and different VEGF isoforms.…”

Section: Discussionsupporting

confidence: 92%

Acute Mechanical Stress in Primary Porcine RPE Cells Induces Angiogenic Factor Expression and in vitro Angiogenesis

Farjood

Ahmadpour

Ostvar

et al. 2020

Preprint

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Background Choroidal neovascularization (CNV) is a major cause of blindness in patients with age-related macular degeneration. CNV is characterized by new blood vessel growth and subretinal fluid accumulation, which results in mechanical pressure on retinal pigment epithelial (RPE) cells. The overexpression of RPE-derived angiogenic factors plays an important role in inducing CNV. In this work, we investigated the effect of mechanical stress on the expression of angiogenic factors in porcine RPE cells and determined the impact of conditioned medium on in-vitro angiogenesis. Results The goal of this study was to determine whether low levels of acute mechanical stress during early CNV can induce the expression of angiogenic factors in RPE cells and accelerate angiogenesis. Using a novel device, acute mechanical stress was applied to primary porcine RPE cells and the resulting changes in the expression of major angiogenic factors, VEGF, ANG2, HIF-1α, IL6, IL8 and TNF - α, were examined using immunocytochemistry, qRT-PCR, and ELISA. An in vitro tube formation assay was used to determine the effect of secreted angiogenic proteins due to mechanical stress on endothelial tube formation by human umbilical vein endothelial cells (HUVECs). Our results showed an increase in the expression of VEGF, ANG2, IL-6 and IL-8 in response to mechanical stress, resulting in increased in vitro angiogenesis. Abnormal epithelial-mesenchymal transition (EMT) in RPE cells is also associated with CNV and further retinal degeneration. Our qRT-PCR results verified an increase in the expression of EMT genes, CDH2, VIM and FN1, in RPE cells. Conclusions In conclusion, we showed that acute mechanical stress induces the expression of major angiogenic and EMT factors and promotes in vitro angiogenesis, suggesting that mechanical stress plays a role in promoting aberrant angiogenesis in AMD.

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“…3q, s, t) was consistent with previous reports of IL -8 and VEGF induction in human RPE cells after RPE injury [20,21]. It has previously been shown that actin polymerization activation induces IL-8 expression and different VEGF isoforms [22,23]. It is therefore possible that disrupting the actin cytoskeleton activates actin polymerization, leading to the increased expression of IL-8 and different VEGF isoforms.…”

supporting

confidence: 91%

Acute Mechanical Stress in Primary RPE Cells Induces Angiogenic Factor Expression and in vitro Angiogenesis

Vargis

Farjood

Ahmadpour

et al. 2020

Preprint

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Background Choroidal neovascularization (CNV) is a major cause of blindness in patients with age-related macular degeneration. CNV is characterized by new blood vessel growth and subretinal fluid accumulation, which results in mechanical pressure on retinal pigment epithelial (RPE) cells. Overexpressing RPE-derived angiogenic factors plays an important role in inducing CNV. Results The goal of this study was to determine whether low levels of acute mechanical stress during early CNV can induce the expression of angiogenic factors in RPE cells and accelerate angiogenesis. Using a novel device, acute mechanical stress was applied to primary porcine RPE cells and the resulting changes in the expression of major angiogenic factors were examined using immunocytochemistry, qRT-PCR, and ELISA. An in vitro tube formation assay was used to determine the effect of mechanical stress on RPE cells on angiogenesis. Our results showed an increase in the expression of major angiogenic factors in response to mechanical stress, resulting in increased in vitro angiogenesis. Abnormal epithelial-mesenchymal transition (EMT) in RPE cells is also associated with CNV and further retinal degeneration. Our qRT-PCR results verified an increase in the expression of EMT genes in RPE cells. Conclusions In conclusion, we showed that acute mechanical stress induces the expression of major angiogenic and EMT factors and promotes in vitro angiogenesis, suggesting that mechanical stress plays a role in promoting aberrant angiogenesis in AMD.

show abstract

The influence of actin depolymerization induced by Cytochalasin D and mechanical stretch on interleukin-8 expression and JNK phosphorylation levels in human retinal pigment epithelial cells

Cited by 16 publications

References 17 publications

Acute mechanical stress in primary porcine RPE cells induces angiogenic factor expression and in vitro angiogenesis

Acute mechanical stress in primary porcine RPE cells induces angiogenic factor expression and in vitro angiogenesis

Acute Mechanical Stress in Primary Porcine RPE Cells Induces Angiogenic Factor Expression and in vitro Angiogenesis

Acute Mechanical Stress in Primary RPE Cells Induces Angiogenic Factor Expression and in vitro Angiogenesis

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