The influence of 5‐hydroxytryptamine agonists and antagonists on identified sympathetic preganglionic neurones in the rat, <i>in vivo</i>

Lewis, David I.; Coote, John H.

doi:10.1111/j.1476-5381.1990.tb12987.x

Cited by 45 publications

(41 citation statements)

References 25 publications

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“…Experiments were undertaken on twenty-eight male Lewis & Coote, 1990). All three muscle pools were filled with paraffin (BDH, Poole, UK) maintained at 37 'C.…”

Section: Methods Animals and Surgerymentioning

confidence: 99%

Chemical mediators of spinal inhibition of rat sympathetic neurones on stimulation in the nucleus tractus solitarii.

Lewis

Coote

1995

The Journal of Physiology

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1. This study was undertaken to gain more direct evidence of the pathways and neurochemical mediators of a spinally mediated baroreceptor inhibition of sympathetic preganglionic neurones (SPNs). 2. For this purpose, single-pulse electrical stimulation within identified vasodepressor regions of the nucleus tractus solitarii (NTS) was used together with extracellular recordings of single antidromically identified SPNs in the T2 segment of the spinal cord of anaesthetized rats. 3. The actions of agonists and antagonists of inhibitory amino acids on the NTS-induced inhibitions were determined, when they were iontophoretically applied in the vicinity of SPNs via a multibarrel micropipette assembly. 4. Extracellular recordings were made from sixty-nine SPNs. In forty-four SPNs, NTS stimulation elicited a period of inhibition of activity in both spontaneous and 'D,L-homocysteic acid-driven' SPNs with a latency to onset of 60 + 6 ms and a magnitude of 80 + 3%. 5. In six out of eight neurones, the NTS-induced inhibition was reduced by 74 + 16% during the application of the glycine antagonist strychnine (0-10 nA, 5-10 min) with doses that selectively blocked the inhibitory effect of iontophoretically applied glycine. 6. In nine out of nine neurones, the NTS-induced inhibition was reduced by 38 + 6 % during the application of the GABAA antagonist bicuculline (5-15 nA, 4-14 min) with doses that selectively blocked the inhibitory effect of iontophoretically applied GABA. 7. In two SPNs, the actions of strychnine and bicuculline were shown to be additive in blocking the NTS inhibition. 8. The selective GABAB antagonists,[6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25] and CGP 55845A

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“…Experiments were undertaken on twenty-eight male Lewis & Coote, 1990). All three muscle pools were filled with paraffin (BDH, Poole, UK) maintained at 37 'C.…”

Section: Methods Animals and Surgerymentioning

confidence: 99%

Chemical mediators of spinal inhibition of rat sympathetic neurones on stimulation in the nucleus tractus solitarii.

Lewis

Coote

1995

The Journal of Physiology

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“…It is also apparent, on the basis of the preceding description, that through different receptor populations, 5-HT could have divergent effects on different populations of neurons in the serially connected organization of the central pathways determining the sympathetic and vagal outputs controlling blood pressure. Thus, if 5-HT has simultaneous access to inhibitory 5-HT receptors (such as 5-HT 1A receptors) on sympathetic premotor neurons and stimulatory 5-HT receptors (such as 5-HT 2A receptors) on sympathetic preganglionic neurons, it is not surprising that administration of serotonin can either inhibit or stimulate preganglionic sympathetic nerves (Lewis and Coote, 1990). Moreover, Pickering et al (1994) showed that perfusion of a spinal cord slice preparation with 5-HT induced rhythmic activity in sympathetic preganglionic neurons.…”

Section: -Ht and Blood Pressurementioning

confidence: 99%

Serotonin and Blood Pressure Regulation

et al. 2012

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“…Furthermore, the 5-HT and noradrenaline re-uptake inhibitor, duloxetine, has been shown to produce increases in external urethral sphincter EMG activity that were reversed by the nonselective 5-HT receptor antagonist, methiothepin and by the 5-HT2 receptor subtype selective antagonist, 4-isopropyl-7-methyl -9-(2-hydroxy-1-methylpropoxycarbonyl)- 4,6,6A,7,8,9,10, 10A-octahydroindolo-[4,3-fg] quinoline maleate, (LY53857; Thor & Katofiasc, 1995). An involvement of 5-hydroxytryptaminergic systems in regulation of sympathetic neural activity is also well-established (deGroat & Ryall, 1967;McCall, 1984;Lewis & Coote, 1990;Helke et al, 1991), but the specific receptor subtypes mediating various effects are not well-defined.…”

Section: Introductionmentioning

confidence: 99%

Spinal 5‐HT₂ receptor‐mediated facilitation of pudendal nerve reflexes in the anaesthetized cat

Danuser

Thor

1996

British J Pharmacology

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1 5-Hydroxytryptamine (5-HT) is intimately associated with central sympathetic and somatic control of the lower urinary tract. The sympathetic and somatic innervation of the lower urinary tract is conveyed through efferent axons of the hypogastric and pudendal nerves, respectively. 2 The present study examined the effects of 2,5-dimethoxy-4-iodophenylisopropylamine (DOI), a 5-HT2 receptor subtype-selective agonist, on evoked potentials recorded from the central ends of the hypogastric and pudendal nerves in response to electrical stimulation of afferent fibres in the pelvic and pudendal nerves, respectively. Various spinalization paradigms were employed to localize the site of action. All cats were pretreated with xylamidine (1 mg kg-'), a peripherally-restricted 5-HT2 receptor antagonist. 3 In acute spinal cats, DOI (0.01-3 mg kg', i.v.) reliably produced dose-dependent increases in the pudendal nerve reflex (to 228+31 % of control). These increases were reversed by the 5-HT2 receptorselective antagonist, LY53857 (0.3-3 mg kg-', i.v.). On the other hand, in spinally-intact cats, DOI produced no significant changes in the pudendal reflex. However, within minutes of spinalization of DOI-pretreated cats, a marked increase (to 221+16% of control) in the pudendal reflex was observed which could be reversed by LY53857. No significant effects were observed on hypogastric reflexes in either acute spinal or spinally-intact cats following DOI administration. No effects were seen in either spinally-intact or acute spinal animals when LY53857 was administered as the initial drug. 4 These results indicate that activation of spinal 5-HT2 receptors facilitates pudendal reflexes. In spinally-intact cats, it is hypothesized that DOI activates supraspinal pathways that mediate inhibition of the pudendal reflexes and counteracts the facilitatory effects of spinal 5-HT2 receptor activation.

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The influence of 5‐hydroxytryptamine agonists and antagonists on identified sympathetic preganglionic neurones in the rat, in vivo

Cited by 45 publications

References 25 publications

Chemical mediators of spinal inhibition of rat sympathetic neurones on stimulation in the nucleus tractus solitarii.

Chemical mediators of spinal inhibition of rat sympathetic neurones on stimulation in the nucleus tractus solitarii.

Serotonin and Blood Pressure Regulation

Spinal 5‐HT₂ receptor‐mediated facilitation of pudendal nerve reflexes in the anaesthetized cat

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The influence of 5‐hydroxytryptamine agonists and antagonists on identified sympathetic preganglionic neurones in the rat, in vivo

Cited by 45 publications

References 25 publications

Chemical mediators of spinal inhibition of rat sympathetic neurones on stimulation in the nucleus tractus solitarii.

Chemical mediators of spinal inhibition of rat sympathetic neurones on stimulation in the nucleus tractus solitarii.

Serotonin and Blood Pressure Regulation

Spinal 5‐HT2 receptor‐mediated facilitation of pudendal nerve reflexes in the anaesthetized cat

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Spinal 5‐HT₂ receptor‐mediated facilitation of pudendal nerve reflexes in the anaesthetized cat