The Inflammatory Response to Implantation of Cotton Pellets in the Rat

Penn, G. B.; Ashford, A.

doi:10.1111/j.2042-7158.1963.tb12883.x

Cited by 29 publications

(12 citation statements)

References 13 publications

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“…The time of implantation in relation to adrenalectomy varied and is stated in the text (day 0 = implantation immmediately after adrenalectomy). The granulomas were removed 3 days after implantation of the pellet since they reach maximum weight at about this time (Penn & Ashford, 1963). In one experiment, granulomas were removed after 7 days.…”

Section: Methodsmentioning

confidence: 99%

Effect of adrenalectomy on cotton pellet granuloma formation in the rat

Ashford¹,

Penn²

1965

Journal of Pharmacy and Pharmacology

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The development of granulomas over 3 days was inhibited in adrenalectomised rats when cotton pellets were implanted on the day of adrenalectomy and was potentiated when the pellets were implanted 7 days after adrenalectomy. The initial inhibitory effect of adrenalectomy was not observed when the granulomas were allowed to remain in situ for 7 days instead of 3 days and was completely reversed by treatment with corticosterone.LTHOUGH the actions of adrenal hormones on developing granula-A tion tissue are widely known, there is some confusion about the effect of adrenalectomy upon healing wounds and granuloma formation. Whereas it has been reported that healing was retarded in adrenalectomised animals (Selye, 1947;Clayton & Prunty, 1951) and that the formation of granulomas around turpentine-induced abscesses in the rat was inhibited by adrenalectomy (Taubenhaus & Amromin, 1950), other workers have found that the amount of tissue formed around an implanted ivalon sponge (Pernokas, Edwards & Dunphy, 1957) or around a plastic ring (Jorgensen, 1962) Our observations show that effects of adrenalectomy ranging from inhibition to potentiation of granuloma formation can be elicited in the rat and are dependant upon the time that elapses between adrenalectomy and implantation of the irritant material. Experimental METHODSFemale Wistar rats, 130 and 180 g weight, were bilaterally adrenalectomised through a dorsal incision under ether anaesthesia. Controls were sham operated and non-operated animals ; the latter were given ether only. The room temperature was 27" and the adrenalectomised animals received 0.9% saline to drink instead of tap water, Sterile cotton pellets each weighing between 6 and 10 mg (Johnson and Johnson) were implanted one in each groin under ether anaesthesia. Each batch of pellets was screened for irritancy since they varied in this property. The time of implantation in relation to adrenalectomy varied and is stated in the text (day 0 = implantation immmediately after adrenalectomy).

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Section: Methodsmentioning

confidence: 99%

Effect of adrenalectomy on cotton pellet granuloma formation in the rat

Ashford¹,

Penn²

1965

Journal of Pharmacy and Pharmacology

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“…Male rats (7-weeksold) were anesthetized, and a thread (1 cm in length, weighing 7 mg) was implanted subcutaneously into the dorsum with a 13-G implantation needle (Natsume Co.), using a modified version of a previously established procedure [28]. To assess the effects of galanin on granulation tissue formation and angiogenesis, rat galanin (100 ng/ 100 ll saline) was subcutaneously injected into the site of the cotton thread implantation immediately after implantation, and then once every day thereafter.…”

Section: Induction Of Granulation Tissue By Use Of Cotton Threadsmentioning

confidence: 99%

Expression of galanin and galanin receptor mRNA in skin during the formation of granulation tissue

et al. 2011

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Galanin is a neuropeptide widely distributed in the central and peripheral nervous systems. Although its role in non-neural cells is poorly understood, it is known that during inflammation, the dermis layer of the skin produces and releases galanin. The aim of this report is to study the expression of galanin in granulation tissue. After inducing inflammation by cotton thread implantation, galanin-like immunoreactivity (galanin-LI) in plasma reached a maximum on the third day. Galanin-LI was observed in fibroblast-like cells occurring close to collagen fibers in developing granulation tissue. Furthermore, galanin receptor subtypes 1 and 2 (GALR1 and GALR2)-expressing cells were observed around microvessels and were found to produce desmin. Galanin was injected along the cotton threads immediately after implantation, resulting in rapid formation of granulation tissue, and an increase in the contents of microvessels, indicating a stimulatory effect of galanin on the process of angiogenesis in granulation tissue. The results demonstrate that some galanin was released from fibroblast-like cells during the formation of granulation tissue, and that it stimulated angiogenesis.

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“…The anti-inflammatory activity of the triterpene was investigated using cotton pellet-induced granuloma model (Penn and Ashford, 1963). The rats (220 ± 20 g) were divided (randomly) into four groups of five rats per group.…”

Section: Cotton Pellet-induced Granulomamentioning

confidence: 99%

Anticoagulant and anti-inflammatory activity of a triterpene from Protorhus longifolia stem bark

Mosa¹,

Ndwandwe²,

Cele³

et al. 2015

J. Med. Plants Res.

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This work evaluated the anticoagulant and anti-inflammatory activity of a lanosteryl triterpene (3β-hydroxylanosta-9,24-dien-21-oic acid) isolated from Protorhus longifolia stem bark. Tail bleeding time assay was used to investigate the ex vivo anticoagulant activity. The effect of the triterpene on the thermally induced aggregation of malate dehydrogenase (MDH) and citrate synthase (CS) was studied. The anti-inflammatory activity of the triterpene was investigated using the cotton pellet-induced granuloma model in rats. Granuloma formation was measured following 7 days of oral administration of the experimental rats (two groups) with the triterpene at 50 and 250 mg/kg body weight (b.w). The compound (50 mg/kg) significantly (p < 0.05) increased bleeding time in rats by up to 7 min as compared to 2.5 min observed in the normal control group. It also improved the activity of Hsp70 on MDH and CS aggregation suppression. The reduction of the granuloma formation by up to 40.3% was observed. It is apparent that the triterpene has potential to inhibit the aggregation of proteins.

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The Inflammatory Response to Implantation of Cotton Pellets in the Rat

Cited by 29 publications

References 13 publications

Effect of adrenalectomy on cotton pellet granuloma formation in the rat

Effect of adrenalectomy on cotton pellet granuloma formation in the rat

Expression of galanin and galanin receptor mRNA in skin during the formation of granulation tissue

Anticoagulant and anti-inflammatory activity of a triterpene from Protorhus longifolia stem bark

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