The inflammatory and immune response to Helicobacter pylori infection

Robinson, Karen; Argent, Richard H.; Atherton, John C.

doi:10.1016/j.bpg.2007.01.001

Cited by 158 publications

(159 citation statements)

References 198 publications

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“…Strains possessing the cag pathogenicity island are more likely to be associated with gastric adenocarcinomas than strains that lack it. 34 Furthermore, Figueiredo et al 35 reported In this study, we found that ATP6V0B, NDUFS5, NDUFV2, ATP6V1F and ATP6V1G1 were overlapping genes involved in both the GO terms and pathway identified. Consequently, these genes probably are the potentially important ones.…”

Section: Discussionmentioning

confidence: 52%

Analysis of whole genomic expression profiles ofHelicobacter pylorirelated chronic atrophic gastritis with IL‐1B‐31CC/‐511TT genotypes

Wang

Shen

et al. 2009

J of Digest Diseases

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OBJECTIVE: Many studies have linked cytokine interleukin‐1B gene polymorphisms to H. pylori‐related gastric cancer development. The current study evaluated the characterization of whole genomic expression profiles of the premalignant condition: H. pylori‐related chronic atrophic gastritis (CAG) with IL‐1B‐31CC/‐511TT genotypes. METHODS: IL‐1B‐31/‐511 gene polymorphisms were determined by DNA sequences. RNA was extracted and expression profiles were performed using Agilent human whole genomic oligonucleotide microarrays (G4112F). The expression of three samples with H. pylori infection was compared to that of three samples without H. pylori infection from samples of six CAG patients, all with IL‐1B‐31CC/‐511TT genotypes. Differentially expressed genes related to H. pylori‐induced CAG with IL‐1B‐31CC/‐511TT genotypes were screened and analyzed further by Gene Ontology (GO) and pathway. Validation of the microarray data was performed using qRT‐PCR. RESULTS: A total of 124 differentially expressed genes and 32 GO term annotations were identified between H. pylori positive and negative groups in the six CAG samples with IL‐1B‐31CC/‐511TT genotypes. The signaling pathways identified were oxidative phosphorylation and epithelial cell signaling in H. pylori infection. Five overlapping genes were contained in identified GO terms and pathways: ATP6V0B, NDUFS5, NDUFV2, ATP6V1F and ATP6V1G1. Comparisons of qRT‐PCR data and the previously reported data with the results of gene chips support the validity of our microarray data. CONCLUSION: The H. pylori‐related CAG with IL‐1B‐31CC/‐511TT genotypes has shown to be the more malignant phenotype than H. pylori negative CAG with IL‐1B‐31CC/‐511TT genotypes. Mitochondrial energy metabolism probably plays a crucial role as it is the molecular mechanism of host–bacterial interactions.

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Section: Discussionmentioning

confidence: 52%

Analysis of whole genomic expression profiles ofHelicobacter pylorirelated chronic atrophic gastritis with IL‐1B‐31CC/‐511TT genotypes

Wang

Shen

et al. 2009

J of Digest Diseases

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“…However, there is not a direct correlation between the prevalence of infection and the prevalence of H. pylori related manifestations since only approximately 10-20 % of those infected develop clinical disease. This occurs because the severity of disease manifestations is influenced by multiple variables, including host genetic diversity, environmental factors and H. pylori genetic heterogeneity [4].…”

Section: Introductionmentioning

confidence: 99%

Correlation of Helicobacter pylori Genotypes with Gastric Histopathology in the Central Region of a South-European Country

et al. 2014

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Background Outcome of Helicobacter pylori (H. pylori) infection results from interaction of multiple variables including host, environmental and bacterial-associated virulence factors. Aim This study aimed to investigate the correlation of cagA, cagE, vacA, iceA and babA2 genotypes with gastric histopathology and disease phenotype in the central region of a South-European country. Methods This prospective study involved 148 infected patients (110 female; mean age 43.5 ± 13.4 years) submitted to endoscopy with corpus and antrum biopsies. H. pylori was cultured and DNA extracted from the isolates. Genotypes were determined by PCR. Histopathological features were graded according to the updated Sydney system and OLGA/OLGIM classification. Only patients with single H. pylori genotypes and complete histopathological results were included.Results Antrum samples presented higher degrees of atrophy, intestinal metaplasia, chronic inflammation and neutrophil activity. Genotype distribution was as follows: cagA-31.8 %; cagE-45.9 %; vacA s1a-24.3 %; vacA s1b-19.6 %; vacA s1c-0.7 %; vacA s2-55.4 %; vacA m1-20.9 %; vacA m2-79.1 %; vacA s1m1-18.9 %; vacA s1m2-25.7 %; vacA s2m1-2 %; vacA s2m2-53.4 %; iceA1-33.8 %; iceA2-66.2 %; babA2-12.2 %. CagA genotype was significantly associated with higher degrees of intestinal metaplasia, neutrophil activity, chronic inflammation and OLGIM stages. BabA2 was linked with higher H. pylori density. Strains with vacA s1m1 or vacA s1m1 ? cagA positive genotypes had a significant association with peptic ulcer and vacA s2m2 with iron-deficient anemia. Conclusions cagA, vacA s1m1 and babA2 genotypes are relatively rare in the central region of Portugal. cagApositive strains are correlated with more severe histopathological modifications. This gene is commonly associated

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“…Thus, these antigens are regarded as prospective candidates that may serve as infection markers (Robinson et al, 2007). Various H. pylori antigens, such as CagA, VacA, HspB, FlaA, FlaB and urease subunits (UreA, UreB), have been employed as diagnostic markers of infection (Widmer et al, 1999;Cremonini et al, 2004;Schumann et al, 2006;Zhang et al, 2012;Flores-Luna et al, 2013;Noto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

Khalilpour

Agilan²,

Lee³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Helicobacter pylori antigen was prepared from an isolate from a patient with a duodenal ulcer. Serum samples were obtained from culture-positive H. pylori infected patients with duodenal ulcers, gastric ulcers and gastritis (n=30). As controls, three kinds of sera without detectable H. pylori IgG antibodies were used: 30 from healthy individuals without history of gastric disorders, 30 from patients who were seen in the endoscopy clinic but were H. pylori culture negative and 30 from people with other diseases. OFF-GEL electrophoresis, SDS-PAGE and Western blots of individual serum samples were used to identify protein bands with good sensitivity and specificity when probed with the above sera and HRP-conjugated anti-human IgG. Four H. pylori protein bands showed good (≥ 70%) sensitivity and high specificity (98-100%) towards anti-Helicobacter IgG antibody in culturepositive patients sera and control sera, respectively. The identities of the antigenic proteins were elucidated by mass spectrometry. The relative molecular weights and the identities of the proteins, based on MALDI TOF/ TOF, were as follows: CagI (25 kDa), urease G accessory protein (25 kDa), UreB (63 kDa) and proline/pyrroline-5-carboxylate dehydrogenase (118 KDa). These identified proteins, singly and/or in combinations, may be useful for diagnosis of H. pylori infection in patients.

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The inflammatory and immune response to Helicobacter pylori infection

Cited by 158 publications

References 198 publications

Analysis of whole genomic expression profiles ofHelicobacter pylorirelated chronic atrophic gastritis with IL‐1B‐31CC/‐511TT genotypes

Analysis of whole genomic expression profiles ofHelicobacter pylorirelated chronic atrophic gastritis with IL‐1B‐31CC/‐511TT genotypes

Correlation of Helicobacter pylori Genotypes with Gastric Histopathology in the Central Region of a South-European Country

Antigenic Proteins of Helicobacter pylori of Potential Diagnostic Value

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