The induction of trehalose and glycerol in Saccharomyces cerevisiae in response to various stresses

Li, Lili; Ye, Yanrui; Pan, Li; Zhu, Yi; Zheng, Suiping; Lin, Ying

doi:10.1016/j.bbrc.2009.07.113

Cited by 94 publications

(68 citation statements)

References 27 publications

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“…Glycerol is the well-known compatible solute in S. cerevisiae. Osmophilic yeasts accumulate glycerol to compensate for high osmotic pressure [20,21]. In the present study, the formation of glycerol was found to be high at the growth/logarithmic phase.…”

Section: Discussionsupporting

confidence: 49%

“…Another important reserve carbohydrate and stress protectant for the yeast is trehalose. Trehalose is also considered as one of the most effective saccharines in preventing phase transition in the lipid bilayer and thereby protecting membranes against damages, and considering the relation of intracellular trehalose concentration with the cellular resistance to osmotic stress, trehalose was supposed to act as an osmoprotectant under osmotic stress [20]. In the supplemented medium, trehalose concentration was decreased at the end of the fermentation which shows that the cells are not under stress when compared to the control.…”

Section: Discussionmentioning

confidence: 99%

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Effect of fruit pulp supplementation on rapid and enhanced ethanol production in very high gravity (VHG) fermentation

Reddy

Ryu

Wee

2014

Bioresour. Bioprocess.

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Background: The energy crisis and climate change necessitate studying and discovering of new processes involved in the production of alternative and renewable energy sources. Very high gravity (VHG) fermentation is one such process improvement aimed at increasing both the rate of fermentation and ethanol concentration. The technology involves preparation and fermentation of media containing 300 g or more of dissolved solids per liter to get a high amount of ethanol. Findings: Saccharomyces cerevisiae was inoculated to the very high gravity medium containing 30% to 40% w/v glucose with and without supplementation of three selected fruit pulps (mango, banana, and sapota). The fermentation experiments were carried out in batch mode. The effect of supplementation of 4% fruit pulp/puree on the metabolic behavior and viability of yeast was studied. Significant increase in ethanol yields up to 83.1% and dramatic decrease in glycerol up to 35% and trehalose production up to 100% were observed in the presence of fruit pulp. The fermentation rate was increased, and time to produce maximum ethanol was decreased from 5 to 3 days with increased viable cell count. The physical and chemical factors of fruit pulps may aid in reducing the osmotic stress of high gravity fermentation as well as enhanced ethanol yield.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Effect of fruit pulp supplementation on rapid and enhanced ethanol production in very high gravity (VHG) fermentation

Reddy

Ryu

Wee

2014

Bioresour. Bioprocess.

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“…1), the biomass can drop as low as 4 g liter Ϫ1 (data not shown), suggesting that at 21 M dissolved oxygen, yeast cells are at the edge of their biomass-producing capacities. Additionally, under this condition, there is a strong increase in carbohydrate synthesis (Table 2), which is a landmark of physiological stress responses in microorganisms (34). Altogether, these data suggest that yeast cells are possibly under multifactorial stress under nitrogen-limited conditions with the OUR saturation regime.…”

Section: Discussionmentioning

confidence: 68%

Oxygen Response of the Wine Yeast Saccharomyces cerevisiae EC1118 Grown under Carbon-Sufficient, Nitrogen-Limited Enological Conditions

Aceituno

Orellana

Torres

et al. 2012

Appl Environ Microbiol

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Discrete additions of oxygen play a critical role in alcoholic fermentation. However, few studies have quantitated the fate of dissolved oxygen and its impact on wine yeast cell physiology under enological conditions. We simulated the range of dissolved oxygen concentrations that occur after a pump-over during the winemaking process by sparging nitrogen-limited continuous cultures with oxygen-nitrogen gaseous mixtures. When the dissolved oxygen concentration increased from 1.2 to 2.7 M, yeast cells changed from a fully fermentative to a mixed respirofermentative metabolism. This transition is characterized by a switch in the operation of the tricarboxylic acid cycle (TCA) and an activation of NADH shuttling from the cytosol to mitochondria. Nevertheless, fermentative ethanol production remained the major cytosolic NADH sink under all oxygen conditions, suggesting that the limitation of mitochondrial NADH reoxidation is the major cause of the Crabtree effect. This is reinforced by the induction of several key respiratory genes by oxygen, despite the high sugar concentration, indicating that oxygen overrides glucose repression. Genes associated with other processes, such as proline uptake, cell wall remodeling, and oxidative stress, were also significantly affected by oxygen. The results of this study indicate that respiration is responsible for a substantial part of the oxygen response in yeast cells during alcoholic fermentation. This information will facilitate the development of temporal oxygen addition strategies to optimize yeast performance in industrial fermentations.

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“…[12][13][14][15] Trehalose, a non-reducing disaccharide, works as an energy source in yeasts, bacteria, fungi, invertebrates, insects and plants. 16,17 Many research demonstrated that the higher trehalose accumulated in the yeast cell the higher tolerance against various environmental stresses, for instance ethanol stress, 18 heat stress, 19 saline stress, 20 and various other environmental stresses. 21,22 In S. cerevisiae, concentration of trehalose is regulated by synthesis enzymes and hydrolysis enzymes.…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

et al. 2017

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Lignocellulosic biomass is an attractive low-cost feedstock for bioethanol production. During bioethanol production, Saccharomyces cerevisiae, the common used starter, faces several environmental stresses such as aldehydes, glucose, ethanol, high temperature, acid, alkaline and osmotic pressure. The aim of this study was to construct a genetic recombinant S. cerevisiae starter with high tolerance against various environmental stresses. Trehalose-6-phosphate synthase gene (tps1) and aldehyde reductase gene (ari1) were co-overexpressed in nth1 (coded for neutral trehalase gene, trehalose degrading enzyme) deleted S. cerevisiae. The engineered strain exhibited ethanol tolerance up to 14% of ethanol, while the growth of wild strain was inhibited by 6% of ethanol. Compared with the wild strain, the engineered strain showed greater ethanol yield under high stress condition induced by combining 30% glucose, 30 mM furfural and 30 mM 5-hydroxymethylfurfural (HMF).

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The induction of trehalose and glycerol in Saccharomyces cerevisiae in response to various stresses

Cited by 94 publications

References 27 publications

Effect of fruit pulp supplementation on rapid and enhanced ethanol production in very high gravity (VHG) fermentation

Effect of fruit pulp supplementation on rapid and enhanced ethanol production in very high gravity (VHG) fermentation

Oxygen Response of the Wine Yeast Saccharomyces cerevisiae EC1118 Grown under Carbon-Sufficient, Nitrogen-Limited Enological Conditions

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

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