The Induction of Rampant Dental Caries in Monkeys (Macaca irus)

Bowen, Willis H.

doi:10.1159/000259597

Cited by 75 publications

(32 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the experiment had been in progress for 18 months, one animal in each group died; data from both of these animals is included in the experimental re sults. The monkeys were examined monthly and radiographs were taken on alter nate months [Bowen, 1969b],…”

Section: Animalsmentioning

confidence: 99%

See 1 more Smart Citation

The Cariostatic Effect of Calcium Glycerophosphate in Monkeys

Bowen¹

1972

Caries Res

Self Cite

View full text Add to dashboard Cite

In extension of previous experiments, a study was devised to test the effect on caries activity of adding calcium glycerophosphate to the diet of monkeys. Five experimental and 6 control animals were fed a high carbohydrate diet. Both groups of animals received icing sugar 5 times daily but that fed to the experimental animals contained 1% calcium glycerophosphate. After 30 months it was observed that the control animals had 47 carious lesions and the experimental animals 8 (p < 0.01). All the control animals developed dental caries, whereas 3 of the experimental animals remained caries-free. Higher levels of calcium, and lower concentrations of soluble and insoluble carbohydrate were observed in the plaque of experimental animals than in plaque of the control monkeys. No differences were observed in the protein and total phosphate content. Both the fasting pH values of plaque and those observed 20 min after the application of sucrose were higher in the experimental animals. Slightly higher counts of micro-organisms were found in plaque from the experimental animals.

show abstract

Section: Animalsmentioning

confidence: 99%

“…It was decided to depart from this procedure. The animals received their basic cariogenic diet [Bowen, 1969b], but in addition received powdered sugar supplemented with calcium gly cerophosphate to 1%> 5 times daily. The control animals received a similar diet but their sugar was not supplemented with calcium glycerophosphate.…”

Section: Experimental Regimenmentioning

confidence: 99%

The Cariostatic Effect of Calcium Glycerophosphate in Monkeys

Bowen¹

1972

Caries Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Streptococcus mutans is an oral organism found almost exclusively on the teeth and its presence appears to be correlated with dental caries (Fitzgerald et al, 1960;Krasse, 1966;Bowen, 1969;Loesche et al, 1975). In the development of caries the deposition and adherence of S. mutans on to the hard, enamel tooth surface are important processes.…”

Section: Introductionmentioning

confidence: 99%

The Influence Of Ionic Strength, Ph And A Protein Layer On The Interaction Between Streptococcus Mutans And Glass Surfaces

Abbott¹,

Rutter²,

Berkeley³

1983

Microbiology

View full text Add to dashboard Cite

The initial interaction between Streptococcus mutans and hard surfaces has been investigated using a rotating disc technique. The deposition to clean and BSA-coated glass of two strains of S.mutans, FA-1 (serotype b) and KPSK2 (serotype c), which exhibit different surface properties, was studied. Organisms were harvested from cultures grown in a chemostat at a dilution rate of 0.06 h-l and suspended in NaCl solutions of defined ionic strengths and pH values. The deposition of both strains showed a strong dependence on electrolyte concentration, particularly at low ionic strengths, which was inversely related to the zeta potentials of the organisms. Similarly, the ionic strength at which maximum deposition was first noted (critical coagulation concentration) for the two strains correlated with their relative potentials. Deposition was insensitive to changes in pH at an electrolyte concentration of 0.05 M. The maximum observed deposition did not approach values predicted by theory, suggesting that a further barrier to deposition, other than electrostatic repulsion, might exist. Under all experimental conditions, some of the deposited bacteria were observed to be oscillating, suggesting that they were held at a distance from the collector surface. The cells did not, however, appear to be deposited in a secondary minimum predicted by DLVO theory hence it may be that long-range polymer interactions are also involved in the deposition of these organisms.

show abstract

“…It is now widely accepted that S. mutans is highly effective in inducing experimental caries in a variety of animals such as hamsters, rats, gerbils and even monkeys (1,5,6,10). Several investigators reported that bacteria other than S. mutans also induced caries in hamsters and rats under certain experimental conditions.…”

mentioning

confidence: 99%

Dental Caries Induction in Experimental Animals by Clinical Strains of Streptococcus mutans Isolated from Japanese Children

Hamada

Ooshima

Torii

et al. 1978

Microbiology and Immunology

View full text Add to dashboard Cite

Oral implantation and the cariogenic activity of clinical strains of Streptococcus mutans which had been isolated from Japanese children and labeled with streptomycin-resistance were examined in specific pathogen-free Sprague-Dawley rats. All the seven strains tested were easily implanted and persisted during the experimental period. Extensive carious lesions were produced in rats inoculated with clinical strains of S. mutans belonging to serotypes c, d, e, and f, and maintained on caries-inducing diet #2000. Noninfected rats did not develop dental caries when fed diet #2000. Type d S. mutans preferentially induced smooth surface caries in the rats. Strains of other serotypes primarily developed caries of pit and fissure origin. Caries also developed in rats inoculated with reference S. mutans strains BH-TR and FAlR (type b) that had been maintained in the laboratories for many years. However, the cariogenicity of the laboratory strains was found to have decreased markedly. All three S. sanguis strains could be implanted, but only one strain induced definite fissure caries. Two S. salivarius strains could not be implanted well in the rats and therefore they were not cariogenic. Four different species of lactobacilli also failed to induce dental caries in rats subjected to similar caries test regimen on diet #2000. S. mutans strain MT6R (type c) also induce caries in golden hamsters and ICR mice, but of variable degrees.

show abstract

The Induction of Rampant Dental Caries in Monkeys (Macaca irus)

Cited by 75 publications

References 5 publications

The Cariostatic Effect of Calcium Glycerophosphate in Monkeys

The Cariostatic Effect of Calcium Glycerophosphate in Monkeys

The Influence Of Ionic Strength, Ph And A Protein Layer On The Interaction Between Streptococcus Mutans And Glass Surfaces

Dental Caries Induction in Experimental Animals by Clinical Strains of Streptococcus mutans Isolated from Japanese Children

Contact Info

Product

Resources

About