The induction of interferon by temperature-sensitive mutants of reovirus, UV-irradiated reovirus, and subviral reovirus particles

Reovirus Infection Enhances Expression of Class I MHC Proteins on Human β-Cell and Rat RINm5F cell

“…Our findings also indicate that increased class I MHC protein expression is driven by productive viral infection, because this effect was not seen when virus supernatants used to infect cells were ultracentrifuged or treated with antiserum to deplete or neutralize, respectively, live virus. Reoviruses are known to be potent inducers of IFN-a and -3 production from infected cells (28,29). Because these IFNs increase class I MHC protein expression on human p-cells (8) and RINm5F cells (unpublished observations) in vitro, it can be hypothesized that increased class I MHC protein expression is due to IFN production by reovirus-infected 3-cells.…”

A Study of Events in Chick Cells Infected with Human Adenovirus Type 5 and their Relationship to the Induction of Interferon

“…A related virus, Sindbis virus, on the other hand does not show any simple relationship between virus RNA or ds RNA synthesis and interferon induction (Lockart et al 1968;Atkins & Lancashire, I976 ). An equally confusing pattern of results was obtained using temperature sensitive mutants of reovirus (Lai & Joklik, (1973)-These results together with the ones reported here suggest that the induction of interferon involves parameters of the infected cells that have not yet been recognized and a search for these factors is obviously a high priority for future research in this area.…”

Calf rotavirus: Detection of outer capsid glycoproteins by lectins

“…Purity of virus preparation was checked by polyacrylamide gel electrophoresis of proteins and by electron microscopy. The virus concentration was estimated assuming a specific absorbance of 5.4 (concentration 1 mg/ml; h 260 nm; optical path: 1 cm) and assuming 1.13 X 1013 particles/mg [12].…”