2003
DOI: 10.4049/jimmunol.170.10.5176
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The Induction of HIV Gag-Specific CD8+ T Cells in the Spleen and Gut-Associated Lymphoid Tissue by Parenteral or Mucosal Immunization with RecombinantListeria monocytogenesHIV Gag

Abstract: The induction of mucosal immunity is crucial in controlling viral replication during HIV infection. In this study we compare the ability of a recombinant Listeria monocytogenes that expresses and secretes the HIV Ag Gag to induce CD8+ T cells against this Ag in the spleen, mesenteric lymph nodes, and Peyer’s patches and the ability to provide effector Gag-specific CD8+ T cells to the lamina propria after i.v., oral, or rectal administration of the vaccine. The levels of Ag-specific CD8+-activated T cells were … Show more

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Cited by 44 publications
(30 citation statements)
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“…While a head-to-head comparison of a pSP-D-CD40L-adjuvanted vaccine with other vaccines has not yet been performed, it is instructive to compare these results with those of other studies in BALB/c mice that employed exactly the same CD8 ϩ T-cell target cell (AMQMLKETI peptide-pulsed P815 cells) for either CTL assays or as a stimulator in IFN-␥ ELISPOT assays. By these measures, the results in this report equaled or exceeded those reported for chimpanzee adenovirus 68-based or vaccinia virusbased Gag vaccines (22), a flavivirus Kunjin Gag vaccine (32), or Gag delivered intraperitoneally using live Listeria monocytogenes as a vector (68). Stronger CD8 ϩ T-cell responses using these assays were reported for a vesicular stomatitis virus vector (30) ϩ T-cell responses to a modest degree (25), but the experiments herein failed to detect any significant effects of multimeric soluble CD40L on CD4 ϩ T-cell-proliferative responses and cytokine production.…”
Section: Discussionmentioning
confidence: 57%
“…While a head-to-head comparison of a pSP-D-CD40L-adjuvanted vaccine with other vaccines has not yet been performed, it is instructive to compare these results with those of other studies in BALB/c mice that employed exactly the same CD8 ϩ T-cell target cell (AMQMLKETI peptide-pulsed P815 cells) for either CTL assays or as a stimulator in IFN-␥ ELISPOT assays. By these measures, the results in this report equaled or exceeded those reported for chimpanzee adenovirus 68-based or vaccinia virusbased Gag vaccines (22), a flavivirus Kunjin Gag vaccine (32), or Gag delivered intraperitoneally using live Listeria monocytogenes as a vector (68). Stronger CD8 ϩ T-cell responses using these assays were reported for a vesicular stomatitis virus vector (30) ϩ T-cell responses to a modest degree (25), but the experiments herein failed to detect any significant effects of multimeric soluble CD40L on CD4 ϩ T-cell-proliferative responses and cytokine production.…”
Section: Discussionmentioning
confidence: 57%
“…We observed a loss of both CD4 ϩ and CD8 ϩ T cells in sham-and LM-wt-immunized cats, while LMgag/pND14-Lc-env-immunized cats maintained IEL subpopulations at FIV-naïve control levels. Oral immunization of mice with HIV-Gag recombinant L. monocytogenes has resulted in Gag-specific responses in about 35% of lamina propria CD8 ϩ T cells (58). Induction of such a robust T-cell response in combination with sIgA may prevent the establishment of viral reservoirs and depletion of CD4 ϩ T cells in the intestine.…”
Section: Cd4mentioning
confidence: 99%
“…Several studies using the mouse model have demonstrated the potential of L. monocytogenes as an HIV vaccine vector (24, 30, 43-45, 58, 62, 64). It has been shown that recombinant L. monocytogenes can induce a strong CTL response and long-lived memory response to HIV Gag and that oral administration with recombinant L. monocytogenes stimulates a strong intestinal mucosal cellular immune response (24,45,58,62,64). Given the potential of L. monocytogenes as a biologic vaccine vector against HIV, recombinant L. monocytogenes needs to be investigated in a challenge system.…”
mentioning
confidence: 99%
“…Intracellular-cytokine staining assays were performed essentially as previously described (37). Single-cell splenocyte suspensions were cultured for 6 h in R10 medium supplemented with 5% recombinant interleukin 2 (IL-2) (Hemagen Diagnostic, Inc.) and 5 g per ml of anti-CD28 antibody (no.…”
Section: Methodsmentioning
confidence: 99%