The induction of cytochrome P-450 in the alkane-utilizing yeast Lodderomyces elongisporus: Alterations in the microsomal membrane fraction

Candida maltosa

“…Like the alkane-hydroxylating P450 system, the two other enzymes, membrane-bound FAOD and FALDH, involved in the oxidation of hydrocarbons to the corresponding fatty acids were also induced by growth of C. maltosa on n-alkanes in comparison with the carbon source glycerol (Table 5; Mauersberger et al 1984Mauersberger et al , 1992aShilova et al 1989). All these enzymes were found to be repressed significantly using glucose as growth substrate, although to a different degree.…”

“…It was concluded that the P450 system is distinguished by the lowest oxygen affinity among the O2 activating enzymes involved in alkane assimilation (Schunck et al 1987a). As studied in detail with C. maltosa ( Table 5; Mauersberger et al , 1984Schunck et al 1987b;Ilchenko et al 1989Ilchenko et al , 1991 and with other yeasts (c. tropicalis, Debaryomyces formicarius, Y. lipolytica, Mauersberger et al 1991; see also Miiller et al 199Ia), the cellular P450 content is strongly increased by transition to oxygen-limited growth during cultivation on long-chain n-alkanes. Enhanced P450 formation was also observed maintaining oxygen saturation but inhibiting more selectively the alkane hydroxylation reaction by introducing low concentrations of carbon monoxide into the culture medium (Mauersberger 1985;Schunck et al 1987a,b).…”

“…Moreover, in different degrees, catalase, cytochrome oxidase, cytochrome c peroxidase, and other hemoproteins respond to oxygen limitation (Table 5; Mauersberger et al 1984;Ilchenko et al 1989Ilchenko et al , 1991. Therefore, it may be assumed that derepression of heme biosynthesis plays an important role.…”

“…Fix spheroplasts of C. maltosa cells grown on n-alkanes or glucose, prepared as described by Mauersberger et al (1984), in 3% glutaraldehyde in 50 mM sodium potassium phosphate buffer, pH 7.0, containing 0.6M sorbitol for 30min at room temperature.…”

Production of Valuable Lipophilic Compounds by Using Three Types of Interface Bioprocesses: Solid–Liquid Interface Bioreactor, Liquid–Liquid Interface Bioreactor, and Extractive Liquid-Surface Immobilization System

“…For example, while Candida parasilosis 86 , C. intermedia 87 , and Lodderomyces elongisporus 88 hydroxylate a terminal methyl group of n-alkanes, Candida lipolytica hydroxylates the terminal methyl and C-2 methylene groups 89 . Regarding fungal hydroxylation of n-alkanes, Cladosporium resinae hydroxylates a terminal methyl group 90 , while Cunninghamella blakesleeana hydroxylates both terminal methyl and subterminal methylene groups of n-alkanes 91 .…”