“…It was concluded that the P450 system is distinguished by the lowest oxygen affinity among the O2 activating enzymes involved in alkane assimilation (Schunck et al 1987a). As studied in detail with C. maltosa ( Table 5; Mauersberger et al , 1984Schunck et al 1987b;Ilchenko et al 1989Ilchenko et al , 1991 and with other yeasts (c. tropicalis, Debaryomyces formicarius, Y. lipolytica, Mauersberger et al 1991; see also Miiller et al 199Ia), the cellular P450 content is strongly increased by transition to oxygen-limited growth during cultivation on long-chain n-alkanes. Enhanced P450 formation was also observed maintaining oxygen saturation but inhibiting more selectively the alkane hydroxylation reaction by introducing low concentrations of carbon monoxide into the culture medium (Mauersberger 1985;Schunck et al 1987a,b).…”