“…Although there has been some progress made in targeted therapies for OSCC, to combat this disease at an advanced stage, more effective therapeutic targets are still required [11]. Interestingly, the reticulocalbin RCN2 is present at abnormally high levels in human OSCC cells and tissues as compared to normal cells and tissues [12]. The effect of RCN2 on OSCC cell proliferation and motility was confirmed in our study.…”
Purpose: To investigate the expression of reticulocalbin-2 (RCN2) in human oral squamous cell carcinoma (OSCC) tissues and cell lines and its effects on the proliferation and migration of OSCC cells.Methods: Immunohistochemical (IHC) assays were performed to evaluate the expression of RCN2 in 30 histologically-confirmed OSCC patient tissues and adjacent tissues. Immunoblot assays were performed to examine the expression of RCN2 in an oral mucosal keratinocyte cell line and OSCC cell lines, while the effects of RCN2 on OSCC cell proliferation were assessed by CCK-8 and colony formation assays. The effects of RCN2 on OSCC cell motility were determined using wound closure and Transwell assays. Furthermore, the effect of RCN2 on EGFR/ERK pathway in OSCC cells was evaluated by immunoblot assay.Results: Overexpression of RCN2 occurred in OSCC tissues and cells (p < 0.01). RCN2 also increased the proliferation of OSCC cells and stimulated the motility of OSCC cells in vitro (p < 0.01). These effects occurred as a result of the regulation of EGFR/ERK signaling pathway by RCN2.Conclusion: RCN2 is a potential therapeutic target for OSCC treatment. However, further in vivo studies are required to validate these findings.
“…Although there has been some progress made in targeted therapies for OSCC, to combat this disease at an advanced stage, more effective therapeutic targets are still required [11]. Interestingly, the reticulocalbin RCN2 is present at abnormally high levels in human OSCC cells and tissues as compared to normal cells and tissues [12]. The effect of RCN2 on OSCC cell proliferation and motility was confirmed in our study.…”
Purpose: To investigate the expression of reticulocalbin-2 (RCN2) in human oral squamous cell carcinoma (OSCC) tissues and cell lines and its effects on the proliferation and migration of OSCC cells.Methods: Immunohistochemical (IHC) assays were performed to evaluate the expression of RCN2 in 30 histologically-confirmed OSCC patient tissues and adjacent tissues. Immunoblot assays were performed to examine the expression of RCN2 in an oral mucosal keratinocyte cell line and OSCC cell lines, while the effects of RCN2 on OSCC cell proliferation were assessed by CCK-8 and colony formation assays. The effects of RCN2 on OSCC cell motility were determined using wound closure and Transwell assays. Furthermore, the effect of RCN2 on EGFR/ERK pathway in OSCC cells was evaluated by immunoblot assay.Results: Overexpression of RCN2 occurred in OSCC tissues and cells (p < 0.01). RCN2 also increased the proliferation of OSCC cells and stimulated the motility of OSCC cells in vitro (p < 0.01). These effects occurred as a result of the regulation of EGFR/ERK signaling pathway by RCN2.Conclusion: RCN2 is a potential therapeutic target for OSCC treatment. However, further in vivo studies are required to validate these findings.
“…The optimal management of the clinically N0 neck in patients with oral cavity cancer has been extensively debated, especially for early-stage tumours. 14,15,27,28 Furthermore, there is no consistent conclusion, and management policies vary across institutions. Routine neck dissection has been advocated by some researchers due to the high incidence of occult cervical metastases, especially in the oral tongue subsite, as the status of the cervical lymph nodes is the most crucial prognostic factor in managing OCSCC.…”
Section: Discussionmentioning
confidence: 99%
“…13 However, recent studies have suggested the "watch and wait" option or staging neck dissection as a second procedure for patients with low-risk OCSCC, including those with an invasion depth under 4 mm, good differentiation, no perineural invasion, and an oral tongue subsite. 14,15 These approaches are based on the paradigm that offering a subpopulation of patients a less aggressive approach will decrease the number of perioperative and postoperative complications and offer non-inferior oncological outcomes, including overall survival, DFS, and locoregional control. Nonetheless, a recent meta-analysis by Ibrahim et al concluded that END is better than watchful waiting in early-stage (T1-T2) OCSCC with regard to regional recurrence and the five-year survival rate.…”
ObjectiveElective neck dissection (END) improves outcomes among clinically node‐negative patients with oral cavity squamous cell carcinoma (OCSCC). However, END is of questionable value, considering the potentially higher comorbidities and operative risks in elderly patients.MethodsA retrospective review of all patients older than 65 years of age who were treated for OCSCC at a tertiary care centre between 2005 and 2020 was conducted.ResultsFifty‐three patients underwent primary tumour resection alone, and 71 had simultaneous END. Most primary tumours were located on the mobile tongue. The patients who did not undergo END had a higher mean age (81.2 vs. 75.1 years, P < 0.00001), significantly shorter surgeries, and shorter hospitalizations. Occult cervical metastases were found in 24% of the patients who underwent END. The two groups showed no significant differences in overall survival or recurrence rates. Similar results were shown in a subpopulation analysis of patients older than 75 years.ConclusionForegoing END in elderly patients with no clinical evidence of neck metastases did not result in lower survival rates or higher recurrence rates.
“…The association between the necessity of END and DOI has been frequently analyzed. Nguyen et al (29) included 70 patients with cT1N0 oral SCC, of whom 27 underwent END and 43 were observed. Regional relapse occurred in 16.3% of patients who were observed and in 3.7% patients who underwent surgery.…”
ObjectivesTo compare the oncologic outcomes in patients with cT1N0 tongue squamous cell carcinoma (SCC) who underwent different neck management strategies stratified by sonographic depth of invasion (DOI).MethodsThe included patients were retrospectively enrolled, and divided into two groups: observation (OBS) and elective neck dissection (END). The regional control (RC) and disease-specific survival (DSS) rates were compared and stratified by sonographic DOI.ResultsThe mean sonographic and pathologic DOIs were 3.8 and 3.7 mm, respectively; the two DOIs were significantly correlated (Spearman correlation coefficient 0.974. p <0.001). In patients with sonographic DOI <4.0 mm, the 5-year RC rates were 73 and 89% in the OBS and END groups, respectively, and were not significantly different. However, in patients with sonographic DOI ≥4.0 mm, the 5-year RC rate was significantly different between the OBS (57%) and END (80%) groups (p = 0.031). In patients with sonographic DOI <4.0 mm, the 5-year DSS rates were 79 and 89% in OBS and END groups, respectively, and were not significantly different. However, in patients with sonographic DOI ≥4.0 mm, the 5-year DSS rate was significantly different between the OBS (67%) and END (86%) groups (p = 0.033).ConclusionsSonographic DOI was notably correlated with pathologic DOI. Moreover, there was a significant survival difference between the OBS and END groups in cT1N0 tongue SCC patients with sonographic DOI ≥4.0 mm but not in those with sonographic DOI <4.0 mm. Our study provides a useful method to aid decision-making in the clinical setting for this patient group.
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