The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency

Gao, Zhenyu; Wang, Yufeng; Chen, Guang; Zhang, Anpeng; Yang, Shenglong; Shang, Lianguang; Wang, Danying; Ruan, Banpu; Liu, Chaolei; Jiang, Hongzhen; Dong, Guojun; Zhu, Li; Hu, Jiang; Zhang, Guangheng; Zeng, Dali; Guo, Longbiao; Xu, Guohua; Teng, Sheng; Harberd, Nicholas P.; Qian, Qian

doi:10.1038/s41467-019-13110-8

Cited by 160 publications

(113 citation statements)

References 50 publications

(45 reference statements)

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“…The indica varieties often display higher NUE than the japonica varieties 48 . Increasing studies revealed that robust NUE commonly exhibits superiority in biomass biogenesis, showing as increased plant height, more tillers, and high grain yield 9,10,[49][50][51] . Therefore, the high NUE is associated with enhanced biomass accumulation; but how N promotes biomass production is so far unclear.…”

Section: G T T T C C C G a C C T A Hap_2 A A Helitron C A C C A G C Amentioning

confidence: 99%

MYB61 is regulated by GRF4 and promotes nitrogen utilization and biomass production in rice

Gao

Zhang

et al. 2020

Nat Commun

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Nitrogen (N) is a macronutrient that boosts carbon (C) metabolism and plant growth leading to biomass accumulation. The molecular connection between nitrogen utilization efficiency (NUE) and biomass production remains unclear. Here, via quantitative trait loci analysis and map-based cloning, we reveal that natural variation at the MYB61 locus leads to differences in N use and cellulose biogenesis between indica and japonica subspecies of rice. MYB61, a transcriptional factor that regulates cellulose synthesis, is directly regulated by a known NUE regulator GROWTH-REGULATING FACTOR4 (GRF4), which coordinates cellulosic biomass production and N utilization. The variation at MYB61 has been selected during indica and japonica domestication. The indica allele of MYB61 displays robust transcription resulting in higher NUE and increased grain yield at reduced N supply than that of japonica. Our study hence unravels how C metabolism is linked to N uptake and may provide an opportunity to reduce N use for sustainable agriculture.

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Section: G T T T C C C G a C C T A Hap_2 A A Helitron C A C C A G C Amentioning

confidence: 99%

MYB61 is regulated by GRF4 and promotes nitrogen utilization and biomass production in rice

Gao

Zhang

et al. 2020

Nat Commun

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“…NUE of indica rice varieties is 30%–40% higher than that of japonica rice varieties (Zhang and Chu, 2020). Three genes, NRT1.1B (Hu et al, 2015) ABC1-1 REPRESSOR1 ( ARE1 ) (Wang et al, 2018), and NR2 (Gao et al, 2019), contribute to the divergence in NUE between the two rice subspecies, indicating a promising strategies to improve the NUE of japonica with indica genes. Moreover, overexpression of OsNRT1.1A in rice not only greatly improves grain yield and N utilization, but also significantly shortens the maturation time (Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Rice NIN-LIKE PROTEIN 1 Rapidly Responds to Nitrogen Deficiency and Improves Yield and Nitrogen Use Efficiency

Alfatih

Zhang

et al. 2020

Preprint

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Nitrogen (N) is indispensable for crop growth and yield, but excessive agricultural application of nitrogenous fertilizers has generated severe environmental problems. A desirable and economical solution to cope with these issues is to improve crop nitrogen use efficiency (NUE). Plant NUE has been a focal point of intensive research worldwide, yet much more has to be learned about its genetic determinants and regulation. Here, we show that rice NIN-LIKE PROTEIN 1 (OsNLP1) plays a fundamental role in N utilization. OsNLP1 protein localizes in nucleus and its transcript level is rapidly induced by N starvation. Overexpression of OsNLP1 improves plant growth, grain yield and NUE under different N conditions while knockout of OsNLP1 impairs grain yield and NUE under N limiting conditions. OsNLP1 regulates nitrate and ammonium utilization by cooperatively orchestrating multiple N uptake and assimilation genes. Chromatin immunoprecipitation and yeast-one-hybrid assays show that OsNLP1 can directly bind to the promoter of these genes to activate their expression. Therefore, our results demonstrate that OsNLP1 is a key regulator of N utilization and represents a potential target for improving NUE and yield in rice.One-sentence summaryOsNLP1 rapidly responds to N availability, enhances N uptake and assimilation, and holds great potential in promoting high yield in rice.

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“…OsNR2 from indica rice (9311) exhibited greater NR activity than its corresponding allele in the japonica cultivar (Nipponbare). The introduction of indica OsNR2 into Nipponbare increased its effective tiller number, grain yield, and NUE (Gao et al, 2019). Overexpression of OsPTR9 (OsNPF8.20), OsPTR6 (OsNPF7.3), and OsNPF7.2 improved rice growth and grain yield (Fang et al, 2013(Fang et al, , 2017Fan et al, 2014;Wang J. et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of OsMYB305 in Rice Enhances the Nitrogen Uptake Under Low-Nitrogen Condition

Wang

Yin

et al. 2020

Front. Plant Sci.

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Excessive nitrogen fertilizer application causes severe environmental degradation and drives up agricultural production costs. Thus, improving crop nitrogen use efficiency (NUE) is essential for the development of sustainable agriculture. Here, we characterized the roles of the MYB transcription factor OsMYB305 in nitrogen uptake and assimilation in rice. OsMYB305 encoded a transcriptional activator and its expression was induced by N deficiency in rice root. Under low-N condition, OsMYB305 overexpression significantly increased the tiller number, shoot dry weight and total N concentration. In the roots of OsMYB305-OE rice lines, the expression of OsNRT2.1, OsNRT2.2, OsNAR2.1, and OsNiR2 was up-regulated and 15 NO 3 − influx was significantly increased. In contrast, the expression of lignocellulose biosynthesis-related genes was repressed so that cellulose content decreased, and soluble sugar concentration increased. Certain intermediates in the glycolytic pathway and the tricarboxylic acid cycle were significantly altered and NADH-GOGAT, Pyr-K, and G6PDH were markedly elevated in the roots of OsMYB305-OE rice lines grown under low-N condition. Our results revealed that OsMYB305 overexpression suppressed cellulose biosynthesis under low-nitrogen condition, thereby freeing up carbohydrate for nitrate uptake and assimilation and enhancing rice growth. OsMYB305 is a potential molecular target for increasing NUE in rice.

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The indica nitrate reductase gene OsNR2 allele enhances rice yield potential and nitrogen use efficiency

Cited by 160 publications

References 50 publications

MYB61 is regulated by GRF4 and promotes nitrogen utilization and biomass production in rice

MYB61 is regulated by GRF4 and promotes nitrogen utilization and biomass production in rice

Rice NIN-LIKE PROTEIN 1 Rapidly Responds to Nitrogen Deficiency and Improves Yield and Nitrogen Use Efficiency

Overexpression of OsMYB305 in Rice Enhances the Nitrogen Uptake Under Low-Nitrogen Condition

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