The incorporation of deoxyuridine monophosphate into DNA increases the sister-chromatid exchange yield

Pardo, Emilia G.; Hernández, Pablo; Gutiérrez, Crisanto

doi:10.1016/0014-4827(87)90023-1

Cited by 14 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was confirmed that the SCE method using the incorporation of the 5-bromodeoxyuridine (BrdU) in combination with 2′deoxyuridine (dUrd) and 5-fluoro-2′-deoxyuridine (FdU) increases the sister chromatid exchange yield (Pardo et al, 1987). The application of BrdU can cause a distortion of the chromosome morphology, a lower proportion of metaphase cells per slide, and problems with poor sister chromatid differentiation as a result of an incorrect BrdU concentration (Gerster and Grant, 1989).…”

Section: Discussionmentioning

confidence: 95%

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

Kwaśniewska

Bara

2020

Front. Plant Sci.

View full text Add to dashboard Cite

This study is an example of using 5-ethynyl-2′-deoxyuridine (EdU) for detecting sister chromatid exchanges (SCEs) at chromosomal level. Here we report a detailed protocol for differential labeling sister chromatids in barley (Hordeum vulgare, 2n = 14) cells that is based on the incorporation and simple detection of EdU. The perfect distinguishing of sister chromatids enabled an analysis of the effects of two model agents-maleic acid hydrazide (MH) and gamma rays-on the formation of SCEs. Using this method, we demonstrated the high sensitivity of barley cells to maleic hydrazide, which is expressed as an increased level of SCEs. A gamma ray induced only slightly more SCEs than in the control cells. The possible mechanisms of MH and gamma ray action in respect to distinguishing chromatids using EdU are discussed. Recommendation for SCEs visualization using EdU as an easy and quick method that can be successfully adapted to other plant species and potentially for human genotoxicity studies is presented.

show abstract

Section: Discussionmentioning

confidence: 95%

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

Kwaśniewska

Bara

2020

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…This was virtually a black box, although it was known that replicative forms of geminiviruses were abundant in S-phase cells [ 24 ]. This side of the original project had deep roots into (i) my early work of cell proliferation kinetics and DNA repair in Allium cepa meristems [ 25 , 26 , 27 , 28 , 29 ], and (ii) my previous experience with SV40 T-ag interactions with the host cell. The identification in 1987 of the human homolog of the yeast p34Cdc2 protein [ 30 ], the main cyclin-dependent kinase (CDK) active during the cell cycle triggered the interest in several laboratories to ask whether plant cells would have a similar protein.…”

Section: Early Studiesmentioning

confidence: 99%

A Journey to the Core of the Plant Cell Cycle

Gutiérrez

2022

IJMS

View full text Add to dashboard Cite

Production of new cells as a result of progression through the cell division cycle is a fundamental biological process for the perpetuation of both unicellular and multicellular organisms. In the case of plants, their developmental strategies and their largely sessile nature has imposed a series of evolutionary trends. Studies of the plant cell division cycle began with cytological and physiological approaches in the 1950s and 1960s. The decade of 1990 marked a turn point with the increasing development of novel cellular and molecular protocols combined with advances in genetics and, later, genomics, leading to an exponential growth of the field. In this article, I review the current status of plant cell cycle studies but also discuss early studies and the relevance of a multidisciplinary background as a source of innovative questions and answers. In addition to advances in a deeper understanding of the plant cell cycle machinery, current studies focus on the intimate interaction of cell cycle components with almost every aspect of plant biology.

show abstract

“…However, successfull attempts have beet? made to determine the presence of uracil in DNA both in animal (Richards et al, 1984) and plant cells (Pardo et a!, 1987),…”

Section: Introductionmentioning

confidence: 99%

Expression and occurrence of uracil-DNA glycosylase in higher plants

Bones¹

1993

Physiol Plant

View full text Add to dashboard Cite

Bones, A. M, 1993, Expression and occurrence of uracil-DNA glvcosylase in higher plants, -PhysioL Plant, 88: 682-688.Uracil-DNA glycosylase (UDG) is the first enzyme in the base excision repair pathway for removal of uracil in DNA, DNA repair capacity is likely to be a critical factor in mutagenesis and thereby in the capacity to prevent genetic damiige and unwanted variation. We have studied expression of UDG in 9 higher plant species. The highest expression of UDG was measured in Soianwn tuberosum. A comparison of 6 Soiunum tuberosum cultivars showed that the specific activity ranged from 30 pmol mg ' protein min ' in the cultivar Laila to 80 pmol mg ' protein min ' in the cultivar Ostara, Measurement of UDG in Begonia x cheimanlhu gave no indications of enzyme activity. The possible effects of no or low UDG activity is discussed. In vitro cultures of .Solarium tuberosum and Thymus vut^aris were used to examine the effect of auxin and cytokinin on the UDG activity. Axillary shoots of Solanum tubero.'ium were cultured on medium including 20 variations in hormone concentration. Auxin (1-naphtaleneacetic acid) increased the expression of UDG. Plants cultured on medium supplemented with 3 mg 1 ' 1-naphtaleneacetic acid showed a specific UDG activity which was approximately 3-fold higher than the activity in controls. The cytokinin benzyladenine reduced the specific UDG activity at concentrations in the range 0,25-10 mg L', In vitro cultured Saintpauiia ionantha was used to examine UDG activity during initiation, conditioning and multiplication cycles. In general, highest expression of UDG was measured in the conditioning cycle on hormone free medium. Measurement of UDG expression during single subculture periods, clearly showed that UDG expression may vary over one culture period. Expression of UDG was in general highest three weeks after transfer to fresh medium. Of different seedling organs from 0-to 15-day-old Brassica napus L,, roots and hypocotyls showed the highest UDG activities. In cotyledons a very low and nearly constant specific activity was observed. In 12-day-old seedlings the activity in roots was approximately 20 times higher than the activity in cotyledons,

show abstract

The incorporation of deoxyuridine monophosphate into DNA increases the sister-chromatid exchange yield

Cited by 14 publications

References 33 publications

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

EdU-Based Step-by-Step Method for the Detection of Sister Chromatid Exchanges for Application in Plant Genotoxicity Assessment

A Journey to the Core of the Plant Cell Cycle

Expression and occurrence of uracil-DNA glycosylase in higher plants

Contact Info

Product

Resources

About