The incorporation of analogues of choline into the phospholipids of the larva of the housefly, Musca domestica

Bridges, R.G.; Ricketts, J.

doi:10.1016/0022-1910(70)90092-2

Cited by 23 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies of Cho requirement in insect development demonstrated that Cho analogs in which a methyl group is replaced with an alkyl chain up to five carbons in length incorporate efficiently into phospholipids (12)(13)(14). We reasoned that propargylcholine (propargyl-Cho) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Metabolic labeling and direct imaging of choline phospholipids in vivo

Jao

Roth

Welti

2009

Proc. Natl. Acad. Sci. U.S.A.

214

250

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Metabolic labeling and direct imaging of choline phospholipids in vivo

Jao

Roth

Welti

2009

Proc. Natl. Acad. Sci. U.S.A.

214

250

View full text Add to dashboard Cite

“…When reared on a synthetic diet which contains no added choline the larvae contain low levels of choline in their lipids which must be derived from the egg and from traces present in one or more components of the diet (Bridges & Price;, Bridges & Ricketts, 1970. The majority of such larvae do not pupate, but the addition of choline or certain analogues of choline to the diet enables normal development of the insect to take place (Bridges & Ricketts, 1970). One such analogue is B-methylcholine (B-MeCh), which, when added to the larval diet, is incorporated into lipid in place of choline giving phosphatidyl B-methylcholine.…”

Section: Introductionmentioning

confidence: 99%

The concentration of choline into specific regions of the thoracic ganglion in B-methylcholine fed houseflies

Howes¹,

Bridges²

1981

J Neurocytol

View full text Add to dashboard Cite

When larvae of the housefly, Musca domestica, are reared on a diet in which most of the choline has been replaced by a choline analogue, B-methylcholine, the small amount of choline available to the insect is largely taken up into the nervous system where it is incorporated into phosphatidylcholine and acetylcholine. Using autoradiographic techniques it is shown that, in the thoracic ganglion, the phosphatidylcholine component of this preferentially accumulated choline is not evenly distributed throughout the tissue but that a well-defined pattern of patches of silver grains is found over certain areas of the neuropile. Underlying this pattern are axons and glial processes structurally indistinguishable from the axons and glia of adjacent areas which show a much lighter autoradiographic reaction. It is concluded that the axons which produce this high density of silver graining are cholinergic and have a high affinity for choline.

show abstract

“…The only known function of DMAE and NMAE is to be incorporated into phospholipids at low dietary choline levels (Bridges et al 1965), and ethanolamine is a normal constituent of insect phospholipids (Gilmour, 1961). The results obtained when DMAE, NMAE or ethanolamine are included in the low-choline diet are not easy to interpret, due largely to the absence of detailed information on phospholipid metabolism in D. melanogaster.…”

Section: Resultsmentioning

confidence: 99%

Physiological genetics of melanotic tumours inDrosophila melanogaster: VIII. The role of choline in the expression of the tumour genetu bwand of its suppressor,su-tu

Sparrow

Sang

1974

Genet. Res.

View full text Add to dashboard Cite

The melanotic tumour gene tu bw of Drosophila melanogaster has a specific suppressor su-tu. The genotypes tu bw; +««-*« and tu bw; su-tu show opposite responses, as measured by tumour penetrance, to increasing choline levels in the defined axenic medium. The three major metabolic functions of choline have been examined using additions to the axenic medium to determine which biochemical pathway(s) are different in the two genotypes. It is concluded that the opposite strain responses are due to changes in the pattern of phospholipid synthesis, and that the gene product of the su-tu gene probably functions in this area of metabolism.

show abstract

The incorporation of analogues of choline into the phospholipids of the larva of the housefly, Musca domestica

Cited by 23 publications

References 16 publications

Metabolic labeling and direct imaging of choline phospholipids in vivo

Metabolic labeling and direct imaging of choline phospholipids in vivo

The concentration of choline into specific regions of the thoracic ganglion in B-methylcholine fed houseflies

Physiological genetics of melanotic tumours inDrosophila melanogaster: VIII. The role of choline in the expression of the tumour genetu bwand of its suppressor,su-tu

Contact Info

Product

Resources

About