The inactivation of infused noradrenaline by the cat spleen

Release of noradrenaline from the cat spleen by sodium deprivation

“…Increase in the concentration of cocaine to 10 ,ug/ml almost completely blocked uptake in normal and in treated slices. Since cocaine specifically blocks uptake of noradrenaline into the sympathetic nerves of the spleen (Gillespie & Kirpekar, 1965), and since it blocked uptake in the treated slices in a similar manner, we would like to conclude that 3H-noradrenaline uptake also occurred inside the sympathetic nerves after their noradrenaline content was severely reduced by sodium deprivation.…”

“…The effect of guanethidine and phenoxybenzamine on the efflux of noradrenaline induced by sodium deprivation Since sodium deprivation inhibits the uptake of noradrenaline, experiments were done to study the effects of agents such as phenoxybenzamine and guanethidine, which also prevent uptake of noradrenaline (Furchgott & Kirpekar, 1963;Gillespie & Kirpekar, 1965;Iversen, 1965). Guanethidine, in addition to preventing uptake, markedly blocks the release of noradrenaline by nerve stimulation (Maxwell, Plummer, Schneider, Povalski & Daniel, 1960).…”

Release of noradrenaline from the cat spleen by potassium

“…A great many investigations (Burn & Rand, 1958;Whitby, Axelrod & Weil-Malherbe, 1961; Furchgott, Kirpekar, Rieker & Schwab, 1963;Gillespie & Kirpekar, 1965;Gillespie & Kirpekar, 1966a, b) suggest that the noradrenaline released from sympathetic nerves is rapidly inactivated by reincorporation into the nerve endings. In addition, Iversen & Kravitz (1966), Kirpekar & Misu (1967) and Kirpekar & Wakade (1968) showed that sodium ions are necessary for the uptake of infused noradrenaline by the rat heart and cat spleen.…”

PROCEEDINGS OF THE BRITISH PHARMACOLOGICAL SOCIETY, 8TH‐9TH‐10TH July, 1970

“…It is concluded that phentolamine stimulates the myocardium by a mechanism involving the sympathetic nervous system, perhaps by protecting released noradrenaline against " tissue inactivation " (Gillespie & Kirpekar, 1965 After the intravenous injection of pentazocine in man some workers (Lal, Savidge & Chhabra 1969) have recorded a transient fall in arterial pressure followed by a rise to levels higher than those of the control period. Others (Jewitt, Maurer & Hubner, 1970) did not observe the initial fall but noted a transient reduction in cardiac output followed by a prolonged rise in systemic and pulmonary arterial pressures.…”