The in vivo performance of an enzyme-assisted self-assembled peptide/protein hydrogel

Williams, Richard J.; Hall, Thomas; Glattauer, Veronica; White, John; Pasic, Paul; Sørensen, Annette; Waddington, Lynne J.; McLean, Keith M.; Currie, Peter D.; Hartley, Patrick G.

doi:10.1016/j.biomaterials.2011.03.078

Cited by 75 publications

(93 citation statements)

References 36 publications

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“…Transgenic Zebrafish Lines-Morpholinos were injected into embryos of the Tg (BAC smyhc1:gfp) i108 transgenic line (16) crossed to tg(bact2-Egfpcaax) pc10 (17). Whole Mount Imaging (Lateral Views)-Embryos were fixed in 4% paraformaldehyde for 3 h at 4°C, washed in PBS, and imaged.…”

Section: Suppression Subtraction Library Production and Salmon Refeedmentioning

confidence: 99%

Stac3 Is Required for Myotube Formation and Myogenic Differentiation in Vertebrate Skeletal Muscle

Bower

serrana

Cole

et al. 2012

Journal of Biological Chemistry

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Background: Stac3, an uncharacterised gene, was identified by its high expression levels in vertebrate muscle. Results: Knockdown of stac3 inhibited zebrafish myofibrillar protein assembly and differentiation in C2C12 cells by perturbing Akt signaling and cell cycle exit. Conclusion: Stac3 is a novel regulator of myogenic differentiation. Significance: Identifying new genes controlling myogenic differentiation will allow greater understanding of processes leading to muscular diseases.

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Section: Suppression Subtraction Library Production and Salmon Refeedmentioning

confidence: 99%

Stac3 Is Required for Myotube Formation and Myogenic Differentiation in Vertebrate Skeletal Muscle

Bower

serrana

Cole

et al. 2012

Journal of Biological Chemistry

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“…Peptide hydrogels have been actively exploited for a variety of biomedical applications such as cell culture, regenerative medicine, controlled drug and gene delivery [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15]. Most peptide hydrogels arise from the entanglement of selfassembled nanoobjects such as nanofibers and nanotubes.…”

mentioning

confidence: 99%

Redox modulated hydrogelation of a self-assembling short peptide amphiphile

Cao

Fan

et al. 2012

Chin. Sci. Bull.

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Hydrogels resulting from the self-assembly of small peptides are smart nanobiomaterials as their nanostructuring can be readily tuned by environmental stimuli such as pH, ionic strength and temperature, thereby favoring their practical applications. This work reports experimental observations of formation of peptide hydrogels in response to the redox environment. Ac-I 3 K-NH 2 is a short peptide amphiphile that readily self-assembles into long nanofibers and its gel formation occurs at concentrations of about 10 mmol/L. Introduction of a Cys residue into the hydrophilic region leads to a new molecule, Ac-I 3 CGK-NH 2 , that enables the formation of disulfide bonds between self-assembled nanofibers, thus favoring cross-linking and promoting hydrogel formation. Under oxidative environment, Ac-I 3 CGK-NH 2 formed hydrogels at much lower concentrations (even at 0.5 mmol/L). Furthermore, the strength of the hydrogels could be easily tuned by switching between oxidative and reductive conditions and time. However, AFM, TEM, and CD measurements revealed little morphological and structural changes at molecular and nano dimensions, showing no apparent influence arising from the disulfide bond formation.peptide amphiphiles, self-assembly, hydrogelation, redox stimuli Citation:Cao C H, Cao M W, Fan H M, et al. Redox modulated hydrogelation of a self-assembling short peptide amphiphile.

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“…The physical hydrogels undergo a profound gel/solution/gel transition upon application and removal of shear, allowing a preformed hydrogel to be delivered as a liquid [22,23]. Alternatively, the peptides can be injected in vivo as a solution with the assembly/gelation triggered by physiological conditions such as temperature, biocatalysis and ionic strength [24][25][26].…”

Section: Self-assembling Peptides As Biomaterials For In Vivo Applicamentioning

confidence: 99%

“…A wide range of techniques have been used to characterise these systems spectroscopically, optically and mechanically at each stage of the assembly process [23,30,32,36,37]. Individual subunit interactions have been characterised by UV-vis spectroscopy, in which the emission wavelength changes during aggregate formation.…”

Section: Self-assembly Of Peptide Materialsmentioning

confidence: 99%

“…The ECM contains proteins rich in structure, such as [35,[103][104][105][106][107][108] laminin and fibronectin, which can be attached to artificial biomaterials either covalently or physically. Whole proteins have been incorporated into SAP scaffolds: permanently through the use of hydrophobic fibrils to allow stable inclusion of whole basement membrane proteins [23]; or temporarily, utilising the electrostatic interactions of charged residues, producing zero order slow release system [40]. Peptide amphiphile systems have been shown to self-assemble in the presence of, and subsequently actively bind, heparin through the inclusion of a heparin binding motif, whilst retaining its bioactivity [41].…”

Section: Functionalisation Of Peptide Materialsmentioning

confidence: 99%

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Self-Assembled Peptides: Characterisation and In Vivo Response

Nisbet

Williams

2012

Biointerphases

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The fabrication of tissue engineering scaffolds is a well-established field that has gained recent prominence for the in vivo repair of a variety of tissue types. Recently, increasing levels of sophistication have been engineered into adjuvant scaffolds facilitating the concomitant presentation of a variety of stimuli (both physical and biochemical) to create a range of favourable cellular microenvironments. It is here that self-assembling peptide scaffolds have shown considerable promise as functional biomaterials, as they are not only formed from peptides that are physiologically relevant, but through molecular recognition can offer synergy between the presentation of biochemical and physio-chemical cues. This is achieved through the utilisation of a unique, highly ordered, nano- to microscale 3-D morphology to deliver mechanical and topographical properties to improve, augment or replace physiological function. Here, we will review the structures and forces underpinning the formation of self-assembling scaffolds, and their application in vivo for a variety of tissue types.

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The in vivo performance of an enzyme-assisted self-assembled peptide/protein hydrogel

Cited by 75 publications

References 36 publications

Stac3 Is Required for Myotube Formation and Myogenic Differentiation in Vertebrate Skeletal Muscle

Stac3 Is Required for Myotube Formation and Myogenic Differentiation in Vertebrate Skeletal Muscle

Redox modulated hydrogelation of a self-assembling short peptide amphiphile

Self-Assembled Peptides: Characterisation and In Vivo Response

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