The in vivo bone formation by mesenchymal stem cells in zein scaffolds

Tu, Jianwei; Wang, Huajie; Li, Huiwu; Dai, Kerong; Wang, Jin‐Ye; Zhang, Xiaoling

doi:10.1016/j.biomaterials.2009.04.054

Cited by 103 publications

(91 citation statements)

References 29 publications

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“…When about 4 mL of bone marrow was harvested, the needle was moved from the rabbit with continuous shaking of the syringe to avoid cruor. 21 Five mL of bone marrow (containing 1 mL heparin) was collected in a sterile 15 mL test tube. The harvested bone marrow was immediately put into centrifuge in a test tube and concentrated at 2000 rpm for 20 minutes.…”

Section: Isolation and Culture Of Autologous Bmscsmentioning

confidence: 99%

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Wang

He²,

Bi³

et al. 2013

IJN

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Purpose: Poly(lactic-co-glycolic acid) (PLGA) is excellent as a scaffolding matrix due to feasibility of processing and tunable biodegradability, yet the virgin scaffolds lack osteoconduction and osteoinduction. In this study, nano-hydroxyapatite (nHA) was coated on the interior surfaces of PLGA scaffolds in order to facilitate in vivo bone defect restoration using biomimetic ceramics while keeping the polyester skeleton of the scaffolds. Methods: PLGA porous scaffolds were prepared and surface modification was carried out by incubation in modified simulated body fluids. The nHA coated PLGA scaffolds were compared to the virgin PLGA scaffolds both in vitro and in vivo. Viability and proliferation rate of bone marrow stromal cells of rabbits were examined. The constructs of scaffolds and autogenous bone marrow stromal cells were implanted into the segmental bone defect in the rabbit model, and the bone regeneration effects were observed. Results: In contrast to the relative smooth pore surface of the virgin PLGA scaffold, a biomimetic hierarchical nanostructure was found on the surface of the interior pores of the nHA coated PLGA scaffolds by scanning electron microscopy. Both the viability and proliferation rate of the cells seeded in nHA coated PLGA scaffolds were higher than those in PLGA scaffolds. For bone defect repairing, the radius defects had, after 12 weeks implantation of nHA coated PLGA scaffolds, completely recuperated with significantly better bone formation than in the group of virgin PLGA scaffolds, as shown by X-ray, Micro-computerized tomography and histological examinations. Conclusion: nHA coating on the interior pore surfaces can significantly improve the bioactivity of PLGA porous scaffolds.

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Section: Isolation and Culture Of Autologous Bmscsmentioning

confidence: 99%

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Wang

He²,

Bi³

et al. 2013

IJN

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“…26 It is generally acknowledged that higher porosity and larger pore size result in better in vivo bone ingrowth, 27 and that porosity about 70%, pore size of 100-500 µm, and mechanical strength similar to natural bone are ideal scaffold characteristics. 28 All the scaffolds in the present study exhibited appropriate physical structures with volume porosities higher than 70% and pore diameters of 150-350 µm.…”

Section: Discussionmentioning

confidence: 99%

Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold

Cao¹,

Duan²,

Wang³

et al. 2012

IJN

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Abstract:The purpose of this study was to investigate the influence of nano-sized β-tricalcium phosphate (β-TCP) on the biological performance of poly (lactic acid) (PLA) composite scaffolds by using in vitro degradation and an in vivo model of heterotopic bone formation. Nano-sized β-TCP (nβ-TCP) was prepared with a wet grinding method from micro-sized β-TCP (mβ-TCP), and composite scaffolds containing 0, 10, 30, or 50 wt% nβ-TCP or 30 wt% mβ-TCP were generated using a freeze-drying method. Degradation was assessed by monitoring changes in microstructure, pH, weight, and compressive strength over a 26-week period of hydrolysis. Composite scaffolds were processed into blocks, and implanted into muscular pockets of rabbits after loading with recombinant human bone morphogenetic protein-2 (rhBMP-2). New bone formation was evaluated based on histological and immunohistochemical analysis 2, 4, and 8 weeks after implantation. The in vitro results indicated that the buffering effect of nβ-TCP was stronger than mβ-TCP, which was positively correlated with the content of nβ-TCP. The in vivo findings demonstrated that nβ-TCP enhanced the osteoconductivity of the scaffolds. Although composite scaffolds containing 30% nβ-TCP exhibited similar osteoconductivity to 50% nβ-TCP, they had better mechanical properties than the 50% nβ-TCP scaffolds. This study supports the potential application of a composite scaffold containing 30% nβ-TCP as a promising scaffold for bone regeneration.

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“…As a major storage protein, Zein is composed of about 45-50% of the total protein in corn. It was demonstrated to be a promising biomaterial with good biocompatibility for tissue engineering [18][19][20][21] and was also used for the preparation of microspheres for constant release of drugs. 22) To be a promising drug delivery system, it is important to explore the pharmacokinetics of drug-loaded Zein in situ gel.…”

Section: Doxorubicin-loaded Zein In Situ Gel For Interstitial Chemothmentioning

confidence: 99%

Doxorubicin-Loaded Zein <i>in Situ</i> Gel for Interstitial Chemotherapy

Cao

Geng

et al. 2012

CHEMICAL & PHARMACEUTICAL BULLETIN

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A novel drug delivery system of doxorubicin (DOX)-loaded Zein in situ gel for interstitial chemotherapy was investigated in this study. The possible mechanisms of drug release were described according to morphological analysis by optical microscopy and scanning electronic microscope (SEM). In vitro and in vivo anti-tumor activity studies showed that DOX-loaded Zein in situ gel was superior to DOX solution. Local pharmacokinetics in tumor tissue was studied by quantitative analysis with confocal laser scanning microscopy (CLSM) combined with microdialysis technology. A pharmacokinetics mathematical model of DOX-loaded Zein in situ gel in tumors was then built.

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The in vivo bone formation by mesenchymal stem cells in zein scaffolds

Cited by 103 publications

References 29 publications

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold

Doxorubicin-Loaded Zein <i>in Situ</i> Gel for Interstitial Chemotherapy

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The in vivo bone formation by mesenchymal stem cells in zein scaffolds

Cited by 103 publications

References 29 publications

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Enhancing the bioactivity of Poly(lactic-co-glycolic acid) scaffold with a nano-hydroxyapatite coating for the treatment of segmental bone defect in a rabbit model

Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized &beta;-tricalcium phosphate scaffold

Doxorubicin-Loaded Zein <i>in Situ</i> Gel for Interstitial Chemotherapy

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Degradation and osteogenic potential of a novel poly(lactic acid)/nano-sized β-tricalcium phosphate scaffold