The In vivo Antioxidant Protective Activity of Mangifera indica Cold Aqueous Leaf Extract in Drosophila Melanogaster

Abstract

Objective: To evaluate in vivo antioxidant activity of Mangifera indica cold aqueous leaf extract. 
 Methods: A number of 50 adult flies were exposed to graded concentrations of Mangifera indca cold aqueous leaf extract, 2.5 mg/10 g diet, 5 mg/10 g diet and 10 mg/10 g diet for 5 days. Each concentration was prepared in 200 µl of distilled water and replicated five times. 10 g diet with 200 µl distilled water served as control. Mortality reading was taken at 24 hours interval. The flies were homogenized, centrifuged and the supernatant was used to assay for Glutathione-S-transferase (GST), Catalase (CAT) and Total thiol content.
 Results: The % mortality of flies after 5 days showed 32.5%, 0%, 15.5% and 37% in the control (10 g diet with 200 µl of distilled water), 2.5 mg/10 g diet, 5 mg/10 g diet and 10 mg/ 10 g diet respectively. There was elevation in total thiol content and high GST and CAT activity in 2.5 mg/10 g diet and 5 mg/10 g diet treated flies.
 Conclusion: The 100% and 85% survival of 2.5 mg/10 g and 5 mg/10 g diet-treated flies respectively and increase of fly antioxidant system after 5 days exposure at these concentrations may suggest protective activity of Mangifera indica in D. melanogaster.

Amaranth Leaf Extract Protects Against Hydrogen Peroxide Induced Oxidative Stress in Drosophila Melanogaster

“…However, most of their antioxidant activity is from polyphenol compounds such as avonoids, iso avones, avones, anthocyanins, catechin and isocatechin (16)(17)(18). Intake of compounds containing exogenous antioxidants is associated with an enhancement of endogenous antioxidant defenses (9,11). This has also been seen in this study with the increase in the in vivo DPPH free radical scavenging activity seen in Drosophila ies fed on Amaranth leaf extracts.…”

“…Exogenous antioxidants can also modulate endogenous enzyme activity (5). For example, catalase activity is increased in Drosophila ies fed on mango tree leaf extracts (11). However, in this study the increase in catalase activity in ies fed on Amaranth extract was not signi cant.…”

“…To determine the in vivo antioxidant activity, catalase enzyme activity and resistance to oxidative stress, groups of Drosophila ies were fed on the respective diets for 5 days (11).…”

Inclusion of Natural Antioxidants of Mango Leaves in Porous Ceramic Matrices by Supercritical CO2 Impregnation

“…Mango ( Mangiferina indica L.) from the Anacardiaceae family is a native fruit from Southeast Asia which is grown mainly in tropical regions in more than 87 countries [ 1 ]. Its exploitation produces a high amount of agro-industrial waste, such as seeds, peels, leaves and steam bark, derived from the activity of pruning and industrial fruit processing.…”