The in vitro renal cell toxicity of some unconventional anticancer phenanthroline-based platinum(II) complexes

Ng, Neville; Wu, Ming; Myers, Simon J.; Aldrich‐Wright, Janice R.

doi:10.1016/j.jinorgbio.2017.11.021

Cited by 9 publications

(6 citation statements)

References 47 publications

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“…Our previous study reported that IC 50 of imatinib in SQUU-A cells calculated by RTCA system and WST-8 assay were 4 µM and 60 µM, respectively (sensitivity of 15 times) (6,7). Other research groups reported that IC 50 of cisplatin in HK-2 cells calculated by RTCA system and WST-8 assay were 0.76 µM and 25 µM, respectively (sensitivity of 33 times) (30,31). These previous data supported our data to show validity.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of IC50 levels immediately after treatment with anticancer reagents using a real‑time cell monitoring device

et al. 2019

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A real-time cell-monitoring analysis (RTCA) system was previously developed based on the change in impedance when cells attach and spread in a culture dish coated with a gold microelectrode array. However, the potential applications of this system have not yet been fully demonstrated. The purpose of this study was to test the utility of the RTCA system to determine the cytotoxicity of four anticancer agents in carcinoma cells. The results were compared with those of the conventional WST-8 assay at the endpoint to determine the potential of the RTCA system as a new real-time assay method to evaluate cytotoxicity. iCELLigence was used as the RTCA system in this study. Suspensions of oral squamous cell carcinoma (OSCC) cell lines were seeded (2x10 4 cells/well) onto the E-plate (the culture plate of the iCELLigence system). After 24 h of culture, anticancer agents were added to each well, and changes in electrical impedance (cell index, CI) were recorded for another 72 h of culture. Cell proliferation was detected in real-time by the RTCA device in an automated, high throughput manner. Then, the IC 50 profiles of the four anticancer agents were calculated based on the real-time cell index values. The results indicated that the RTCA system was useful in evaluating cytotoxic reactions immediately after the addition of the anticancer agents as it was able to record the data in real-time. Furthermore, the IC 50 levels measured by the real-time assay were lower than those measured by the endpoint assay. Thus, RTCA systems can be used to evaluate chemotherapeutic agents in cancer cells as well as their side effects in normal cells.

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Section: Discussionsupporting

confidence: 91%

Evaluation of IC50 levels immediately after treatment with anticancer reagents using a real‑time cell monitoring device

et al. 2019

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“…The IC 50 values obtained from the live cell imaging were lower than from MTS both for SN38 and SN38@F127@PDA . Similar findings were also noted for several other drugs using real-time cell analysis, 82–84 which shows the importance to use different methods when determine the effect of new therapies to avoid generating misleading results.…”

Section: Resultssupporting

confidence: 74%

Size-tuneable and immunocompatible polymer nanocarriers for drug delivery in pancreatic cancer

et al. 2022

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“…Such IC 50 value is greater than other reported Pt-based anticancer agents, however, it is still largely below millimolar range, thereby, showing significant cytotoxic activity against HCT116 cells. [62][63][64] This result led us to Notably, the time-dependent WST-8 viability showed that the anticancer activity of 1 was observed with a clear viability decay up to ~50% within 24 h while other metal complexes did not exhibit any kinetics of cytotoxicity on HCT116 cells (Fig. S11 †).…”

Section: Synthesis and Characterization Of First-row Transition Metal Complexesmentioning

confidence: 92%

Bioinspired nonheme iron complex that triggers mitochondrial apoptotic signalling pathway specifically for colorectal cancer cells

Lee

Jin

et al. 2022

Chem. Sci.

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The in vitro renal cell toxicity of some unconventional anticancer phenanthroline-based platinum(II) complexes

Cited by 9 publications

References 47 publications

Evaluation of IC50 levels immediately after treatment with anticancer reagents using a real‑time cell monitoring device

Evaluation of IC50 levels immediately after treatment with anticancer reagents using a real‑time cell monitoring device

Size-tuneable and immunocompatible polymer nanocarriers for drug delivery in pancreatic cancer

Bioinspired nonheme iron complex that triggers mitochondrial apoptotic signalling pathway specifically for colorectal cancer cells

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