The in-Vitro Production of Antibody Lymphocytes

DNA polymerase activity as an index of lymphocyte stimulation: studies in Down's syndrome

“…Changes similar to those that occur on PHA stimulation are observed on stimulating lymphocytes with antigens to which a person is known to have been sensitized previously (33,34) and in mixed leukocyte reactions where histocompatibility antigens appear to be the stimulating factors (14). PHA-treated lymphocytes have been shOwn to synthesize immunoglobulins (35)(36)(37), specific antibodies (36,37), and interferon (38). PHA-stimulated cells are also capable of destroying human fibroblasts in vitro (39).…”

The Place of the Lymphocyte in the Reticulo-endothelial System: A Study of the in vitro Effect of Prednisolone on Lymphocytes

“…They have been shown to be capable of DNA synthesis (44), and to produce antibody (32). PH A cells have also been shown to synthesize DNA (8,30) and to produce antibody (18,27). If these two cell types are identical, and the products of the antigenic stimulation of the lymphocyte, then it would seem that the lymphoid germinal centre is an antibody-producing organ rather than a major site of lympho poiesis as is generally supposed.…”

The stimulation of DNA synthesis in cultures of rabbit lymph node and spleen cell suspensions by homologous cells

“…These and other workers have demonstrated similar in vitro changes in peripheral leukocytes brought about by antigens to which the subject had been immunized (17)(18)(19)(20)(21)(22)(23)(24)(25)(26), by extracts of autologous tissues in patients with autoimmune diseases (15,26,27), antisera from rabbits immunized to human leukocytes (28,29), and by phytohemaggiutinin M (12,25,(30)(31)(32)(33)(34)(35)(36)(37). The response to the latter was found to be most intense.…”