The in vitro micronucleus assay for detection of cytogenetic effects induced by mutagen-carcinogens: comparison with the in vitro sister-chromatid exchange assay

Lasne, C; Gu, Z.W.; Venegas, W.; Chouroulinkov, I

doi:10.1016/0165-1161(84)90103-1

Cited by 70 publications

(24 citation statements)

References 44 publications

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“…On the other hand, the use of cytochalasin B is controversial; Matsuoka et al [1992] recommended avoiding cotreatment with other bioactive chemicals since having almost all cells continuously dividing and serving as target cells facilitates the evaluation of chemical clastogenicity. In the same way, Kirsch-Volders et al [2000] indicate that there are neither clear advantages [Fritzenschaf et al, 1993;Stopper et al, 1994] 2B Methyl methanesulfonate MMS 66-27-3 Al U W [Lasne et al, 1984;Beranek, 1990;Zhang et al, 1995;Elhajouji et al, 1997;Jiang et al, 1999] …”

Section: Use Of Cytochalasin Bmentioning

confidence: 84%

Using CTLL‐2 and CTLL‐2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test

Meintières

Biola

Pallardy

et al. 2003

Environ and Mol Mutagen

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In vitro assays for chromosome aberrations (i.e., in vitro micronucleus and in vitro metaphase analysis tests) frequently produce false-positive or exaggerated-positive results. Our previous work suggested that apoptosis interferes with these tests, producing misleading results. These previous studies were conducted by performing the in vitro micronucleus test in CTLL-2 cells and a CTLL-2 cell derivative stably transfected with the apoptosis inhibitor gene bcl2. In the present study, these previous observations were extended by examining micronucleus induction with a larger number of compounds in both CTLL-2 and CTLL-2 bcl2 cells and measuring apoptosis with annexin V-FITC. Both cell lines were treated with different classes of compounds that were anticipated to be exclusively apoptosis inducers, or compounds known to be clastogens or aneugens, some of which were anticipated to be both genotoxic and apoptotic. We were able to confirm that compounds that are only apoptogenic induced micronuclei in CTLL-2 but not CTLL-2 bcl2 cells, indicating that the positive responses are due to apoptosis in CTLL-2 cells. Some genotoxins (clastogens and aneugens) did not produce apoptosis by the annexin V assay and gave similar responses in CTLL-2 and CTLL-2 bcl2 cells. Finally, higher responses were induced in CTLL-2 cells compared to CTLL-2 bcl2 cells that were treated with aneugens or clastogens that were also apoptosis inducers, suggesting that the greater response in CTLL-2 cells is a consequence of both genotoxicity and apoptosis. Finally, it was demonstrated that just eliminating CTLL-2 cells having three or more micronuclei from scoring was not adequate for correctly evaluating agents that only produce apoptosis. The results indicate that coupling the in vitro micronucleus test in both CTLL-2 and CTLL-2 bcl2 cells with the measurement of apoptosis is able to distinguish the genotoxic effects of a test compound from its apoptotic potential and is able to avoid interference from apoptosis in the in vitro micronucleus test. These observations may provide the basis for a useful genotoxicity assay.

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Section: Use Of Cytochalasin Bmentioning

confidence: 84%

Using CTLL‐2 and CTLL‐2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test

Meintières

Biola

Pallardy

et al. 2003

Environ and Mol Mutagen

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“…Slides were air-dried, stained with 4,6-diamidino-2-phenylindole (10 mg/l; Sigma, St Louis, USA) for 5 min, and washed in 0.9% NaCl solution. Micronuclei were examined at 4009 magnification using a fluorescence microscope (Axiophot, Zeiss, Germany) and 1,000 binucleated cytokinesis-blocked cells were scored for MN according to criteria published by Lasne et al (1984). All evaluations were performed under blinded conditions and by the same investigator (E.K.).…”

Section: Micronucleus Assaymentioning

confidence: 99%

Radiofrequency electromagnetic fields (UMTS, 1,950 MHz) induce genotoxic effects in vitro in human fibroblasts but not in lymphocytes

Schwarz

Kratochvil

Pilger

et al. 2008

Int Arch Occup Environ Health

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“…Both ethyl nitrosourea (ENU) and EMS induced a linear response up to 100 micronuclei per 1000 cells. Lasne et al (22) also examined the induction of micronuclei in V79 cells. MNNG, MMS, and EMS were examined only at low concentrations.…”

Section: 'Exposure Of 6 Hr (83)mentioning

confidence: 99%

Results of animal studies suggest a nonlinear dose-response relationship for benzene effects.

Parodi¹,

Lutz²,

Colacci³

et al. 1989

Environ Health Perspect

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Considering the very large industrial usage of benzene, studies in risk assessment aimed at the evaluation of carcinogenic risk at low Ievels of exposure are important. Animal data can offer indications about what could happen in humans and provide more diverse information than epidemiological data with respect to doseresponse consideration. We have considered experiments investigating metabolism, short·term genotoxicity tests, DNA adduct formation, and carcinogenicity long-term tests. According to the different experiments, a Saturation of benzene metabolism and benzene effects in terms of genotoxicity seems evident above 30 to 100 ppm. Below 30 to 60 ppm the initiating effect ofbenzene seems tobe linear fora large intervaJ ofdosages, at least judging from DNA adduct formation. Potentiallack of a promoting effect of benzene (below 10 ppm) could generate a sublinear response at nontox.ic levels of ex.posure. This possibility was suggested by epidemiological data in humans and is not confirmed or excluded by our observations with animals.

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The in vitro micronucleus assay for detection of cytogenetic effects induced by mutagen-carcinogens: comparison with the in vitro sister-chromatid exchange assay

Cited by 70 publications

References 44 publications

Using CTLL‐2 and CTLL‐2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test

Using CTLL‐2 and CTLL‐2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test

Radiofrequency electromagnetic fields (UMTS, 1,950 MHz) induce genotoxic effects in vitro in human fibroblasts but not in lymphocytes

Results of animal studies suggest a nonlinear dose-response relationship for benzene effects.

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