The In Vitro Impact of the Herbicide Roundup on Human Sperm Motility and Sperm Mitochondria

Anifandis, George; Amiridis, G.S.; Dafopoulos, Konstantinos; Daponte, Alexandros; Dovolou, Eleni; Gavriil, Eleftherios; Gorgogietas, Vyron A.; Kachpani, Elli; Mamuris, Zissis; Messini, Christina I.; Vassiou, Katerina; Psarra, Anna‐Maria G.

doi:10.3390/toxics6010002

Cited by 54 publications

(52 citation statements)

References 47 publications

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“…Thirty (30) healthy men volunteered for semen analysis for the study during 2016 and gave written informed consent; Institutional Review Board approval of the study was also obtained (protocol number: 2538/15.6.2016). The present study is following previous work concerning the effect of Roundup on human sperm motility and sperm mitochondria [ 14 ]. All of the subjects were living in the agricultural region of Greece, Thessaly.…”

Section: Methodsmentioning

confidence: 99%

“…Portions of 0.5 mL of the total volume of each specimen were centrifuged at 2000 rpm for 5 min and the supernatant from each sample was carefully discarded, while the pellet was resuspended in 1 mL of pre-warmed buffer solution (Gamete Buffer, William Cook Australia PTY LTD ® , Brisbane, Australia) containing glyphosate at a final concentration of 0.36 mg/L. The rationale for the dose of 0.36 mg/L glyphosate was that in the previous work [ 14 ] we used 1 mg/L Roundup at final concentration which corresponded to 0.36 mg/L glyphosate. Therefore, the obtained results from glyphosate will be at least explanatory when compared to the results obtained by Roundup.…”

Section: Methodsmentioning

confidence: 99%

“…The present study is following the aim and scope of previous work concerning the effect of Roundup on human sperm motility and sperm mitochondria [ 14 ]. Considering the correlation between sperm motility and mitochondrial functionality [ 15 ], the aim of the present work is to elucidate and differentiate the role of Roundup from its main constituent, glyphosate.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Glyphosate on Human Sperm Motility and Sperm DNA Fragmentation

Anifandis

Katsanaki

Lagodonti

et al. 2018

IJERPH

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Glyphosate is the active ingredient of Roundup®, which is one of the most popular herbicides worldwide. Although many studies have focused on the reproductive toxicity of glyphosate or glyphosate-based herbicides, the majority of them have concluded that the effect of the specific herbicide is negligible, while only a few studies indicate the male reproductive toxicity of glyphosate alone. The aim of the present study was to investigate the effect of 0.36 mg/L glyphosate on sperm motility and sperm DNA fragmentation (SDF). Thirty healthy men volunteered to undergo semen analysis for the purpose of the study. Sperm motility was calculated according to WHO 2010 guidelines at collection time (zero time) and 1 h post-treatment with glyphosate. Sperm DNA fragmentation was evaluated with Halosperm® G2 kit for both the control and glyphosate-treated sperm samples. Sperm progressive motility of glyphosate-treated samples was significantly reduced after 1 h post-treatment in comparison to the respective controls, in contrast to the SDF of glyphosate-treated samples, which was comparable to the respective controls. Conclusively, under these in vitro conditions, at high concentrations that greatly exceed environmental exposures, glyphosate exerts toxic effects on sperm progressive motility but not on sperm DNA integrity, meaning that the toxic effect is limited only to motility, at least in the first hour.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Effect of Glyphosate on Human Sperm Motility and Sperm DNA Fragmentation

Anifandis

Katsanaki

Lagodonti

et al. 2018

IJERPH

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“…Animal and epidemiological studies published in recent decades point to a potential glyphosate toxicity [10][11][12]. Further, the World Health Organization's International Agency for Research on Cancer concluded that glyphosate is "probably carcinogenic to humans" [13] and Anifandis et al [14,15] demonstrated that glyphosate/PMG induce DNA fragmentation.…”

Section: Introductionmentioning

confidence: 99%

31P NMR Investigations on Roundup Degradation by AOP Procedures

Kudzin

Żyłła

Mrozińska

et al. 2019

Water

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The reactions of (N-(PhosphonoMethyl)Glycine) PMG with H2O2 in homogenous systems were investigated using 31P NMR (Nuclear Magnetic Resonance). These reactions were carried out in two reaction modes: without UV radiation and under UV radiation. The reactions of PMG with H2O2 without UV radiation were carried out in two modes: the degradations of PMG (0.1 mmol) by means of 5–10 molar excess of hydrogen dioxide (PMG-H2O2 = 1:5 and 1:10) and the degradation of PMG (0.1 mmol) in homogenous Fenton reactions (PMG-H2O2-Fe2+ = 1:10:0.05 and 1:10:0.1). All reactions were carried out at ambient temperature, at pH 3.5, for 48 h. The reactions of PMG (in Roundup herbicide composition, 12 mmol) with H2O2 under UV radiation (254 nm) were carried out using 5 × molar excess of H2O2 (60 mmol), in the pH range of 2 ≤ pH ≤ 12, for 6 h. In this mode of PMG oxidation, the splitting of C-P was observed in the ratios dependent on the applied pH of the reaction mixture.

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“…There is significant interest in the potential impact of environmental toxicity, such as fungicides, insecticides, and herbicides, on male reproductive potential, with several existing publications reporting reductions in semen quality [ 1 , 2 , 3 ]. The reproductive toxicity of bisphenol A (BPA) has aroused concern with our widespread use of plastic products.…”

Section: Introductionmentioning

confidence: 99%

Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice

Yuan

Lin

et al. 2018

IJERPH

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Many previous studies have indicated the adverse effects of bisphenol A (BPA) on sperm production and quality; however, the mechanisms underlying BPA male reproductive toxicity have yet to be elucidated. The main purpose of this study was to investigate the effect of perinatal exposure to BPA on the spermatogenic capacity of male offspring, and to explore the possible influence of inflammatory responses in BPA reproductive toxicity. Twenty-one pregnant C57BL/6mice were randomly divided into three groups: a control group, a group receiving 0.2 μg/mL (LBPA), and a group receiving 2 μg/mL of BPA (HBPA), all via drinking water from gestational day 6 to the end of lactation. After weaning, one male mouse was randomly selected from each group (n = 7/group); these three mice were fed a normal diet and drinking water for 1 month. Levels of serum testosterone (T) and tumor necrosis factor (TNF)-α were then measured in all mice. Sperm count and the proportion of sperm malformation were also determined. The levels of Toll-like receptor 4 (TLR4), nuclear factor (NF)-κB, and aryl hydrocarbon receptor (AhR) protein expression in the testis tissue were determined. Analysis showed that the proportion of sperm malformation increased in the LBPA and HBPA groups (p < 0.05). Sperm count significantly decreased only in the HBPA group (p < 0.05), while the levels of serum TNF-α increased in the LBPA and HBPA groups (p < 0.05). Levels of serum T decreased significantly in the HBPA group, compared with controls (p < 0.05). Levels of TLR4 and NF-κB protein expression in the testis were significantly higher in the LBPA and HBPA groups (p < 0.05 or p < 0.01), while AhR protein expression was higher and seminiferous tubules in the testis showed more damage in the HBPA group compared to controls (p < 0.05 and p < 0.01, respectively). Our results showed that perinatal exposure to low or high doses of BPA decreased the capacity for spermatogenesis in male offspring, which may be associated with an inflammatory response activated by the TLR4/ NF-κB and AhR signaling pathways in the testis.

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The In Vitro Impact of the Herbicide Roundup on Human Sperm Motility and Sperm Mitochondria

Cited by 54 publications

References 47 publications

The Effect of Glyphosate on Human Sperm Motility and Sperm DNA Fragmentation

The Effect of Glyphosate on Human Sperm Motility and Sperm DNA Fragmentation

31P NMR Investigations on Roundup Degradation by AOP Procedures

Decreased Capacity for Sperm Production Induced by Perinatal Bisphenol A Exposure Is Associated with an Increased Inflammatory Response in the Offspring of C57BL/6 Male Mice

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