The in vitro effects of dehydroepiandrosterone on human osteoarthritic chondrocytes

Jo, Han-Shin; Park, J. S.; Kim, E. M.; Jung, Mun-Su; Lee, S. H.; Seong, Sang Cheol; Park, S. C.; Kim, H. J.; Lee, Myung Chul

doi:10.1016/s1063-4584(03)00094-3

Cited by 50 publications

(35 citation statements)

References 55 publications

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“…MMP-1 is a commonly detected metalloproteinase in synovial fluid from patients suffering from osteoarthritis (Freemont et al , 1997; Goupille et al , 1998; Kanyama et al , 2000; Yoshihara et al , 2000; Neuhold et al , 2001; Jo et al , 2003; Little et al , 2009). Another degradative enzyme, ADAMTS-4, was reported to be a major aggrecanase in cartilage of mouse and ADAMTS-5 was known to be important in cartilage matrix destruction during osteoarthritis (Stanton et al , 2005; Echtermeyer et al , 2009).…”

Section: Discussionmentioning

confidence: 99%

Luteolin Inhibits the Activity, Secretion and Gene Expression of MMP-3 in Cultured Articular Chondrocytes and Production of MMP-3 in the Rat Knee

Kang

Ryu

Lee

et al. 2014

Biomolecules & Therapeutics

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We investigated whether luteolin affects the gene expression, secretion and activity of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as production of MMP-3 in the rat knee to evaluate the potential chondro-protective effects of luteolin. Rabbit articular chondrocytes were cultured in a monolayer and IL-1β-induced gene expression levels of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), ADAMTS-5 and type II collagen were measured by reverse transcription - polymerase chain reaction (RT-PCR). Effects of luteolin on interleukin-1β (IL-1β)-induced secretion and enzyme activity of MMP-3 in rabbit articular chondrocytes were investigated by western blot analysis and casein zymography, respectively. The effect of luteolin on MMP-3 protein production was also examined in vivo. The results were as follows: (1) luteolin inhibited the gene expression levels of MMP-3, MMP-1, MMP-13, ADAMTS-4 and ADAMTS-5. However, it increased the gene expression level of collagen in rabbit articular chondrocytes; (2) luteolin inhibited the secretion and activity of MMP-3; (3) luteolin inhibited in vivo production of MMP-3 protein. These results suggest that luteolin can regulate the gene expression, secretion and activity of MMP-3, by directly acting on articular chondrocytes.

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Section: Discussionmentioning

confidence: 99%

Luteolin Inhibits the Activity, Secretion and Gene Expression of MMP-3 in Cultured Articular Chondrocytes and Production of MMP-3 in the Rat Knee

Kang

Ryu

Lee

et al. 2014

Biomolecules & Therapeutics

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“…Extraction of RNA and RT-PCR Total RNA was extracted, and reverse transcription and amplification were performed as previously described (Jo et al 2003). Briefly, total RNA was extracted by using Trizol reagent (Gibco-BRL, USA).…”

Section: Chondrogenic Differentiationmentioning

confidence: 99%

Fetal mesenchymal stem cells derived from human umbilical cord sustain primitive characteristics during extensive expansion

Kim

Park

et al. 2008

Cell Tissue Res

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Stem cells of fetal origin lie between embryonic and adult stem cells in terms of potentiality. Because of the ethical controversy surrounding embryonic stem cells and the relatively inferior quality of adult stem cells, the use of fetal stem cells would be an attractive option in future therapeutic applications. Here, we have investigated primitive characteristics of human umbilical-cord-derived fetal mesenchymal stem cells (UC fMSCs) during extensive expansion. We have successfully isolated and cultured UC fMSCs from all UC samples, but with two early fungal contaminations. UC fMSCs proliferated without significant evidence of morphological changes, and the average cumulative population-doubling level was over 25 for about 3 months. UC fMSCs showed the positive expression of several CD markers, known to be related to MSCs, including CD73 (SH-3, 4), CD90 (Thy-1), CD105 (SH-2), CD117 (c-kit), and CD166 (ALCAM). They demonstrated primitive properties throughout the expansion period: multilineage differentiation potentials examined by functional assays, a variety of pluripotent stem cell markers including Nanog, Oct-4, Sox-2, Rex-1, SSEA-3, SSEA-4, Tra-1-60, and Tra-1-81, minimal evidence of senescence as shown by beta-galactosidase staining, and the consistent expression of telomerase activity. These results suggest that UC fMSCs have more primitive properties than adult MSCs, which might make them a useful source of MSCs for clinical applications.

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“…DHEA increased human a1 (I) procollagen and reduced collagenase (MMP-1) gene expression in cultured skin fibroblasts (Lee et al, 2000). In human cultured chondrocytes, DHEA increased type II collagen and TIMP-1 gene expressions, but decreased type I collagen, MMP-1, and MMP-3 expression (Jo et al, 2003). On the other hand, DHEA-S stimulated collagenase and gelatinase production, but did not alter collagen production in rabbit cervical tissue (Sakyo et al, 1987).…”

Section: Discussionmentioning

confidence: 98%

Modulation of Collagen Metabolism by the Topical Application of Dehydroepiandrosterone to Human Skin

Shin¹,

Rhie²,

Park

et al. 2005

Journal of Investigative Dermatology

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Dehydroepiandrosterone (DHEA) and its sulfate conjugate (DHEA-S) are the most abundantly produced human adrenal steroids to be reduced with age. DHEA may be related to the process of skin aging through the regulation and degradation of extracelluar matrix protein. In this study, we demonstrate that DHEA can increase procollagen synthesis and inhibit collagen degradation by decreasing matrix metalloproteinases (MMP)-1 synthesis and increasing tisuue inhibitor of matrix metalloprotease (TIMP-1) production in cultured dermal fibroblasts. DHEA was found to inhibit ultraviolet (UV)-induced MMP-1 production and the UV-induced decrease of procollagen synthesis, probably due to the inhibition of UV-induced AP-1 activity. DHEA (5%) in ethanol:olive oil (1:2) was topically applied to buttock skin of volunteers 12 times over 4 weeks, and was found to significantly increase the expression of procollagen alpha1(I) mRNA and protein in both aged and young skin. On the other hand, topical DHEA significantly decreased the basal expression of MMP-1 mRNA and protein, but increased the expression of TIMP-1 protein in aged skin. We also found that DHEA induced the expressions of transforming growth factor-beta1 and connective tissue growth factor mRNA in cultured fibroblasts and aged skin, which may play a role in the DHEA-induced changes of procollagen and MMP-1 expression. Our results suggest the possibility of using DHEA as an anti-skin aging agent.

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The in vitro effects of dehydroepiandrosterone on human osteoarthritic chondrocytes

Abstract: Our study demonstrates that DHEA has no toxic effect on chondrocytes up to 100 micro M of concentration and has an ability to modulate the imbalance between MMPs and TIMP-1 during OA at the transcription level, which suggest that it has a protective role against articular cartilage loss.

Cited by 50 publications

References 55 publications

Luteolin Inhibits the Activity, Secretion and Gene Expression of MMP-3 in Cultured Articular Chondrocytes and Production of MMP-3 in the Rat Knee

Luteolin Inhibits the Activity, Secretion and Gene Expression of MMP-3 in Cultured Articular Chondrocytes and Production of MMP-3 in the Rat Knee

Fetal mesenchymal stem cells derived from human umbilical cord sustain primitive characteristics during extensive expansion

Modulation of Collagen Metabolism by the Topical Application of Dehydroepiandrosterone to Human Skin

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