The in vitro effects of dehydroepiandrosterone on chondrocyte metabolism

Sun, Jui Sheng; Wu, Chien-Liang; Tsuang, Yang Hwei; Chen, L. T.; Sheu, Sheh Yi

doi:10.1016/j.joca.2005.09.012

Cited by 27 publications

(16 citation statements)

References 49 publications

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“…These effects may be related to increases of nitric oxide (Henrotin et al 1998;Mathy-Hartert et al 2002;Petrov et al 2005;Steinberg et al 1987;Vuolteenaho et al 2001), IL-1 (Tiku et al 1992), IL-6 (Henrotin et al 1998), IL-8 (Henrotin et al 1998, PGE 2 (Henrotin et al 1998;Mathy-Hartert et al 2002), collagenase (Arend et al 1985), MMP-3 and MMP−13 (Sun et al 2006) production, as in chondrocytes. LPS has been shown to stimulate cytokine production markedly, such as that of IL-1β, IL-6, and IL-8, by chondrocytes (Mathy-Hartert et al 2002) and by murine disc cells (Rand et al 1997).…”

Section: Discussionmentioning

confidence: 99%

“…The possible mechanism of the actions of LPS presented here, i.e., whether LPS directly induces cells to produce matrix enzymes or nitric oxide or stimulates the production of cytokines, such as IL-1 and TNF, remains to be elucidated. Although recent studies have utilized LPS to stimulate cartilage degradation, (Mathy-Hartert et al 2002;Sun et al 2006), the mechanisms through which LPS exerts its effects on chondrocytes are still not well characterized. LPS has been shown specifically to activate immunity in peripheral organs through a well described Toll-like receptor (TLR)-dependent signaling pathway (Akira 2003;PalssonMcDermott and O'Neill 2004).…”

Section: Discussionmentioning

confidence: 99%

“…It is often used in vitro to induce degradation of the cartilage matrix to simulate pyrogenic arthritis (de Mattei et al 2002;Henrotin et al 1998;Jasin 1983;Mathy-Hartert et al 2002;Morales et al 1984;Steinberg et al 1987;Sun et al 2006). For example, exposure of chondrocytes to LPS causes a rapid decrease in the content of proteoglycan (PG; Jasin 1983).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of cellular response in bovine intervertebral disc cells and articular chondrocytes: effects .of lipopolysaccharide on proteoglycan metabolism

Aota

Imai

et al. 2006

Cell Tissue Res

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Lipopolysaccharide (LPS) induces matrix degradation and markedly stimulates the production of several cytokines, i.e., interleukin-1beta, -6, and -10, by disc cells and chondrocytes. We performed a series of experiments to compare cellular responses of cells from the bovine intervertebral disc (nucleus pulposus and annulus fibrosus) and from bovine articular cartilage to LPS. Alginate beads containing cells isolated from bovine intervertebral discs and articular cartilage were cultured with or without LPS in the presence of 10% fetal bovine serum. The DNA content and the rate of proteoglycan synthesis and degradation were determined. In articular chondrocytes, LPS strongly suppressed cell proliferation and proteoglycan synthesis in a dose-dependent manner and stimulated proteoglycan degradation. Compared with articular chondrocytes, nucleus pulposus cells responded in a similar, although less pronounced manner. However, treatment of annulus fibrosus cells with LPS showed no significant effects on proteoglycan synthesis or degradation. A slight, but statistically significant, inhibition of cell proliferation was observed at high concentrations of LPS in annulus fibrosus cells. Thus, LPS suppressed proteoglycan synthesis and stimulated proteoglycan degradation by articular chondrocytes and nucleus pulposus cells. The effects of LPS on annulus fibrosus cells were minor compared with those on the other two cell types. The dissimilar effects of LPS on the various cell types suggest metabolic differences between these cells and may further indicate a divergence in pathways of LPS signaling and a differential sensitivity to exogenous stimuli such as LPS.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of cellular response in bovine intervertebral disc cells and articular chondrocytes: effects .of lipopolysaccharide on proteoglycan metabolism

Aota

Imai

et al. 2006

Cell Tissue Res

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“…Chondrocytes were released from articular cartilage after being digested with 0.2% pronase for 1 h, followed by digestion in PBS containing 0.25% trypsin and 0.05% EDTA at 37 C for 1 h. After washing with PBS, cartilage was further digested for 3 h with PBS containing 0.1% collagenase in DMEM [25]. After removing undigested cartilage using a 70-lm nylon sieve, the chondrocytes were collected by centrifugation, washed with PBS, and cultured in 75 cm 2 plastic flasks containing 15 mL DMEM to which 10% FBS, L-glutamine (2.0 mM) and penicillin/streptomycin (100 U/mL, 100 lg/mL) were added.…”

Section: Chondrocyte Culturesmentioning

confidence: 99%

Hyaluronan differently modulates TLR‐4 and the inflammatory response in mouse chondrocytes

et al. 2012

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Hyaluronic acid (HA) may exert different action depending on its degree of polymerization. Small HA fragments induce proinflammatory responses, while highly polymerized HA exerts a protective effect in inflammatory pathologies such as rheumatoid arthritis. In both cases the toll-like receptor 4 (TLR-4) seems to be involved in the modulation of the inflammation process. The aim of this study was to investigate the influence of short HA oligosaccharides (HA 4-mers) and high molecular weight HA (HMWHA) in the inflammatory response in normal mouse chondrocytes. Messenger RNA and related protein levels were measured for TLR-4, tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and interleukin-18 (IL-18) in cells with and without the addition of HA. NF-kB activation was also evaluated. 4-mer HA treatment produced a significant up-regulation of all parameters considered while HMWHA did not exert any activity in untreated cells although it was able to reduce the effects of 4- mers HA significantly. Specific TLR-4 small interference RNA (siRNA) was used to confirm TLR-4 as the target of HA action. This study suggests that HA may modulate proinflammatory cytokines via its different degree of polymerization and inflammatory action may be modulated as a result of the interaction between HA and TLR-4.

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“…Finally, studies in rheumatoid arthritis and brain injury models have shown that DHEA can modulate the expression of COX-2 mRNA or PGE 2 synthesis (Malik et al, 2003;Sun et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Resolution of Colitis-Associated Inflammation

Hennebert¹,

Marie-Agnès²,

Morfin³

2012

Colitis

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The in vitro effects of dehydroepiandrosterone on chondrocyte metabolism

Abstract: Our study demonstrates that DHEA has an ability to modulate the imbalance between MMPs and PGE2 in the neonatal chondrocytes which suggest that it has a potential protective role against articular cartilage damage.

Cited by 27 publications

References 49 publications

Comparison of cellular response in bovine intervertebral disc cells and articular chondrocytes: effects .of lipopolysaccharide on proteoglycan metabolism

Comparison of cellular response in bovine intervertebral disc cells and articular chondrocytes: effects .of lipopolysaccharide on proteoglycan metabolism

Hyaluronan differently modulates TLR‐4 and the inflammatory response in mouse chondrocytes

Resolution of Colitis-Associated Inflammation

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