The in vitro effect of vancomycin on multidrug resistant Staphylococcus aureus from hospital currency notes

Adegoke,; Anthony, A; Okoh,; Anthony, Iain

doi:10.5897/ajmr11.441

Cited by 5 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data indicated that Ofloxacin showed the highest percentage reduction in antibiotics resistance following the treatment with acredine orange, with no reduction observed for Cefuroxime, Erythromycin and Augmentin. This observation is in line with the reports of earlier researchers [23][24][25]. The notable reduction in antibiotic resistance of the species to these antibiotics could therefore be attributed to the presence of resistance plasmid genes, which were eliminated after plasmid curing.…”

Section: Discussionsupporting

confidence: 92%

Antibiotics Resistance and Plasmid Curing Studies of Staphylococcus aureus associated with Wound Infection amongst Patients Accessing University of Port Harcourt Teaching Hospital, Rivers State, Nigeria

Alexander¹,

T.²,

Ugboma³

2023

IJHMS

View full text Add to dashboard Cite

Staphylococcus aureus is a common hospital and community-acquired pathogen known to be frequently associated with wound infections, and has become important for its increasing level of resistance to antibiotics. Resistance of Staphylococcus aureus to antibiotics could be as a result of the presence of plasmids or resistance genes. The need to carry out plasmid curing of the isolates is very essential. The aim of the study was therefore to determine the antibiotics resistance pattern of staphylococcus aureus associated with wound infection amongst patients accessing university of Port Harcourt Teaching Hospital, Rivers State, Nigeria. A total of 150 specimens from different types of wounds (traumatic wound, caesarean section, scrotal wound, surgical wounds, burns, diabetic foot, and plastic surgery) were collected from the Hospital for a period of six months and processed for isolation of S. aureus, following standard microbiological procedures. The specimens were cultured on sterile Mannitol Salt Agar (MSA) plates and incubated at 37ºC for 24 hours. Antibiotic susceptibility tests of the isolates was performed using the Kirby-Bauer disc diffusion method, following CLSI guidelines. Plasmid genes of the isolates were cured using acridine orange. The study showed a decrease in antibiotic resistance of 17.3% for ofloxacin (OFL), 13.8% for gentamicin (GEN) and 3.5% for both ceftazidime (CAZ) and ceftriatone (CTR) after plasmid curing, with no apparent change in resistance for the rest of the antibiotics. The results of this study represent serious public health concerns, thus emphasizing the need for proper wound management.

show abstract

Section: Discussionsupporting

confidence: 92%

Antibiotics Resistance and Plasmid Curing Studies of Staphylococcus aureus associated with Wound Infection amongst Patients Accessing University of Port Harcourt Teaching Hospital, Rivers State, Nigeria

Alexander¹,

T.²,

Ugboma³

2023

IJHMS

View full text Add to dashboard Cite

show abstract

Antimicrobial Resistance Patterns and Plasmid Profiles of Staphylococcus Aureus Isolated from Different Clinical Specimens in Saudi Arabia

Jaran¹

2017

ESJ

View full text Add to dashboard Cite

The aims of this study were to investigate antibiotic resistance patterns and plasmid profile of antibiotic resistant Staphylococcus strains isolated from clinical specimens, and to find out a possible relationship between plasmids profile and antibiotic sensitivity patterns. Staphylococcus strains were isolated from different clinical specimens from different hospitals and primary health care centers. Antimicrobial susceptibility of Staphylococcus isolates was determined using disc diffusion method against 10 commonly used antimicrobial drugs. Plasmid DNA was extracted from lysed Staphylococcus cells using Plasmid Miniprep kit, and visualized using Agarose gel electrophoresis. The results showed that isolated strains of Staphylococcus were resistant to Penicillin (61%), Erythromycin (45%), Chloramphenicol (21%) and Co-trimoxazole (15%). Plasmid analysis of clinical isolates showed the presence of 0 to 3 plasmids with size range of 2 to 31 Kb, as compared to control Staphylococcus aureus ATCC 25923 which showed 4 plasmids (size range of 2 to 21 Kb). The results obtained in this study showed no direct correlation between the patterns of antibiotic resistance and plasmid profiles.

show abstract

Bacteriological Evaluation of Hand Contact Surfaces at Bus Terminals in Uyo Metropolis

John¹,

Adegoke²

2018

J Pure Appl Microbiol

View full text Add to dashboard Cite

The in vitro effect of vancomycin on multidrug resistant Staphylococcus aureus from hospital currency notes

Cited by 5 publications

References 0 publications

Antibiotics Resistance and Plasmid Curing Studies of Staphylococcus aureus associated with Wound Infection amongst Patients Accessing University of Port Harcourt Teaching Hospital, Rivers State, Nigeria

Antibiotics Resistance and Plasmid Curing Studies of Staphylococcus aureus associated with Wound Infection amongst Patients Accessing University of Port Harcourt Teaching Hospital, Rivers State, Nigeria

Antimicrobial Resistance Patterns and Plasmid Profiles of Staphylococcus Aureus Isolated from Different Clinical Specimens in Saudi Arabia

Bacteriological Evaluation of Hand Contact Surfaces at Bus Terminals in Uyo Metropolis

Contact Info

Product

Resources

About