The in vitro and in vivo antibacterial activities of uniflorous honey from a medicinal plant, Scrophularia ningpoensis Hemsl., and characterization of its chemical profile with UPLC-MS/MS

Lin, Tianxing; Huang, Lei; Cheng, Ningna; Wang, Yuzhen; Zhang, Ning; Huang, Shaokang; Wu, Yuanhua; Chen, Tianbao; Su, Songkun; Lin, Yan

doi:10.1016/j.jep.2022.115499

Cited by 11 publications

(5 citation statements)

References 91 publications

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“…The H 2 O 2 concentration in honeys ranged from 7.26 to 47.56 µg H 2 O 2 g −1 (Figure 1C). These data agree with the values obtained by other authors, ranging from 1 to 47.2 µg g −1 [15,[39][40][41]. We did not find any relationship between the amount of H 2 O 2 and the botanical origin of honeys, unlike other researchers [42] who described that honeydew and chestnut honey contained large amounts of H 2 O 2 , while acacia, heather, and rape honeys had a lower capacity to produce hydrogen peroxide.…”

Section: Hydrogen Peroxide Concentrationsupporting

confidence: 91%

Relationships among Hydrogen Peroxide Concentration, Catalase, Glucose Oxidase, and Antimicrobial Activities of Honeys

Osés,

Rodríguez,

Valencia

et al. 2024

Foods

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Honey is a natural sweetener made by bees that exhibits antimicrobial activity, mainly related to its H2O2 content. The aim of this work was to research the H2O2 concentration of 24 Spanish honeys from different botanical origins, studying their possible correlation with glucose oxidase (GOx), catalase (CAT), and anti-Staphylococcus aureus activities (minimal inhibition concentration (MIC), minimal bactericidal concentration (MBC), and percentage of inhibition at 5% (w/v) honey against Staphylococcus aureus), as well as possible correlations among all the analyzed parameters. The results showed that the H2O2 concentration did not depend on the botanical origin of the honeys. There were neither correlations between the H2O2 concentration and the activities of GOx and CAT, nor between GOx and antimicrobial activity. However, CAT and antimicrobial activities were positively correlated. Therefore, CAT could be successfully used as a possible marker of the antimicrobial activity of honeys against Staphylococcus aureus. Furthermore, a linear regression model has been fitted to explain the antimicrobial activity from CAT and GOx activity and H2O2 concentration. Although H2O2 is one of the compounds involved in honey’s antibacterial activity, this capacity also strongly depends on other honey components (such as low water activity, acidity, osmolarity, and phenolic compounds). The very high anti-Staphylococcus aureus activity exhibited by all samples could be interesting for commercial honey-based formulations also helping to promote local beekeeping.

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Section: Hydrogen Peroxide Concentrationsupporting

confidence: 91%

Relationships among Hydrogen Peroxide Concentration, Catalase, Glucose Oxidase, and Antimicrobial Activities of Honeys

Osés,

Rodríguez,

Valencia

et al. 2024

Foods

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“…Over the past decade, intense screenings of other honey types have been performed to identify new sources of medical-grade honey that would be attractive for the pharmaceutical industry [11]. In many cases, the antibacterial activity of newly described honey types is often compared to that of manuka honey, with different levels of MGO or different unique manuka factor (UMF) [17][18][19][20][21][22][23][24][25]. However, the mechanism of antibacterial action in all analysed nonmanuka honeys is based on the action of hydrogen peroxide (H 2 O 2 ).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of physicochemical parameters and antibacterial properties of New Caledonian honeys

Bucekova,

Godocikova,

Gueyte

et al. 2023

PLoS ONE

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Honey is an attractive natural product with various health benefits. A few honey-based commercial products have successfully been adopted in clinics to improve wound healing. However, screening of other potential sources of medical-grade honey, in particular, honeys from territories with high floral species diversity and high endemicity, is highly needed. The goal of this study was to characterise the physicochemical and antibacterial properties of New Caledonian honey samples (n = 33) and to elucidate the major mechanism of their antibacterial action. Inhibitory antibacterial activity of honeys against Staphylococcus aureus and Pseudomonas aeruginosa was determined with a minimum inhibitory concentration (MIC) assay. Enzymatic activity of glucose oxidase and the content of hydrogen peroxide (H2O2) in honey samples were analysed. Furthermore, total protein content of honeys together with their electrophoretic protein profiles were also determined in the study. The antibacterial efficacy of 24% of the tested honey samples was slightly superior to that of manuka honey with unique manuka factor 15+. The antibacterial activity of catalase-treated honey sample solutions was significantly reduced, suggesting that H2O2 is a key antibacterial compound of diluted honeys. However, the kinetic profiles of H2O2 production in most potent honeys at a MIC value of 6% was not uniform. Under the experimental conditions, we found that a H2O2 concentration of 150 μM in diluted honeys is a critical concentration for inhibiting the growth of S. aureus. In contrast, 150 μM H2O2 in artificial honey solution was not able to inhibit bacterial growth, suggesting a role of phytochemicals in the antibacterial activity of natural honey. In addition, the continuous generation of H2O2 in diluted honey demonstrated an ability to counteract additional bacteria in re-inoculation experiments. In conclusion, the tested New Caledonian honey samples showed strong antibacterial activity, primarily based on H2O2 action, and therefore represent a suitable source for medical-grade honey.

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“…For example, Ginkgo biloba exocarp extract was reported to downregulate the expression of the MRSA biofilm-associated factors sarA and sigB [ 97 ]. Similarly, Scrophularia ningpoensis honey can down-regulate the expression of genes icaA , icaD , sarA , agrA , and sigB in S. aureus [ 98 ].…”

Section: Promising Targets In the Regulation Of S Aureus ...mentioning

confidence: 99%

Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics

Wu,

Wang,

Xiong

et al. 2024

Biofilm

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The in vitro and in vivo antibacterial activities of uniflorous honey from a medicinal plant, Scrophularia ningpoensis Hemsl., and characterization of its chemical profile with UPLC-MS/MS

Cited by 11 publications

References 91 publications

Relationships among Hydrogen Peroxide Concentration, Catalase, Glucose Oxidase, and Antimicrobial Activities of Honeys

Relationships among Hydrogen Peroxide Concentration, Catalase, Glucose Oxidase, and Antimicrobial Activities of Honeys

Characterisation of physicochemical parameters and antibacterial properties of New Caledonian honeys

Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics

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