The in-vitro activity of a new highly active quinolone, DU-6859a

Jolley, Alan; Andrews, J. M.; Brenwald, N P; Wise, R.

doi:10.1093/jac/32.5.757

Cited by 24 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5 In the present study, this drug had significantly lower MICs for S. pneumoniae with resistance to levofloxacin, compared with the other quinolones tested, and these findings support previous reports showing a similar potency of this drug against levofloxacinresistant S. pneumoniae with defined multiple mutations within both gyrase A and topoisomerase IV. 9,10 Gemifloxacin is also potent against such strains with double mutations both in gyrase A and topoisomerase IV.…”

Section: Discussionsupporting

confidence: 92%

In vitro activity of sitafloxacin against clinical strains of Streptococcus pneumoniae with defined amino acid substitutions in QRDRs of gyrase A and topoisomerase IV

Touyama

Higa

Nakasone

et al. 2006

Journal of Antimicrobial Chemotherapy

View full text Add to dashboard Cite

Objectives: Fluoroquinolone-resistant Streptococcus pneumoniae are increasing worldwide rapidly. In vitro activities of sitafloxacin were evaluated against clinical isolates of S. pneumoniae resistant to levofloxacin (MIC of levofloxacin 4 mg/L), which were characterized genetically. Methods:The quinolone resistance determining regions (QRDRs) of gyrA, gyrB, parC and parE of these strains were analysed by PCR-based sequencing. MICs of sitafloxacin and other quinolones were determined by a microdilution broth method. Conclusions: These findings warrant further studies to evaluate the usefulness of sitafloxacin in the treatment of levofloxacin-resistant S. pneumoniae infection.

show abstract

Section: Discussionsupporting

confidence: 92%

In vitro activity of sitafloxacin against clinical strains of Streptococcus pneumoniae with defined amino acid substitutions in QRDRs of gyrase A and topoisomerase IV

Touyama

Higa

Nakasone

et al. 2006

Journal of Antimicrobial Chemotherapy

View full text Add to dashboard Cite

show abstract

“…17 To overcome this problem, STFX, a recently developed quinolone, is potent against Gram-positive cocci as well as Gram-negative rods, and excellent activities of these drugs against S. pneumoniae have been reported. 18 In the present study, STFX had significantly lower MICs against S. pneumoniae with resistance to LVFX, compared with other quinolones tested, and these findings support previous reports that show the potency of this drug against LVFX-resistant S. pneumoniae with defined multiple mutations within both the gyrase A and topoisomerase IV genes. 19,20 Interestingly, three (#13, #29 and #30) of five strains (#13, #18, #29, #30 and #35) that showed a higher MIC (0.5 mg l À1 ) against LVFX-resistant strains possessed Glu85Lys substitutions in gyrA.…”

Section: Discussionsupporting

confidence: 92%

Comparison of drug sensitivity and genotypes of clinically isolated strains of levofloxacin-resistant Streptococcus pneumoniae obtained from Okinawa Island, the Japanese main island and Hong Kong

et al. 2011

View full text Add to dashboard Cite

The prevalence of fluoroquinolone-resistant Streptococcus pneumoniae is increasing worldwide. In the present study, a comparison of drug sensitivity and genotypes of clinically isolated strains of levofloxacin (LVFX)-resistant S. pneumoniae obtained from Hong Kong, Okinawa Island and the Japanese main island (Honshu) was performed. MICs of quinolones (LVFX, tosufloxacin, ciprofloxacin, gatifloxacin and sitafloxacin (STFX)) and other antibiotics (penicillin G, cefcapene, cefditoren, clarithromycin and azithromycin) were determined by a microdilution broth method according to the Clinical and Laboratory Standards Institute Standards. The quinolone-resistance determining regions (QRDRs) of gyrA, gyrB, parC and parE of these strains were analyzed by PCR-based sequencing. All 40 strains tested had more than one amino-acid substitution in the QRDRs of gyrA, gyrB, parC or parE. Although there seemed to be some clonality in strains obtained from Hong Kong, there was no clonality in strains obtained from Okinawa and Japan. Strains obtained from Hong Kong, Okinawa Island and the Japanese main island were genetically different by pulsed-field gel electrophoresis analysis. The range of MIC values of STFX against isolates resistant to LVFX (MIC 4-32 mg l À1 ) was 0.12-0.5 mg l À1 , and MIC 80 values of STFX against LVFX-resistant isolates were 0.25 mg l À1 . This study suggests that LVFX-resistant S. pneumoniae is similar in all three locations and STFX is potent against LVFX-resistant S. pneumoniae with multiple mutations in QRDRs of gyrase A and topoisomerase IV.

show abstract

“…A microbiological assay has been accepted as a general method to measure antibiotics in food and livestock products, [8][9][10] but shows low detection speciˆcity and poor quantiˆcation. Alternatively, high pressure liquid chromatography (HPLC) and gas liquid chromatography (GLC) methods have been well demonstrated to measure antibiotics quantitatively and sensitively.…”

mentioning

confidence: 99%

Simultaneous Measurement of Fluoroquinolones in Eggs by a Combination of Supercritical Fluid Extraction and High Pressure Liquid Chromatography

Shim

Lee

Kim

et al. 2003

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Simultaneous detection of the fluoroquinolone antibiotics ciprofloxacin, enrofloxacin, ofloxacin, and norfloxacin in eggs by a combination of supercritical fluid extraction (SFE) and high pressure liquid chromatography (HPLC) was studied. Lipid matrices that have been considered to result in poor extraction and isolation of fluoroquinolones in eggs were removed first by SFE with supercritical CO(2) alone, and then the fluoroquinolones were extracted by SFE with supercritical CO(2) containing 20% (v/v) methanol for HPLC analysis. A time-course study of the extraction of lipid matrices of eggs suggested that the SFE method successfully removed the matrices within 20 min. When the fluoroquinolones added to control eggs were extracted by SFE, the extraction efficiency was similar to that by the solvent extraction method, giving the recovery percentages from 83 to 96% in a 40 min-extraction time. The fluoroquinolones extracted from eggs by SFE were analyzed simultaneously by HPLC equipped with a fluorescence detector with detection sensitivity at about 10 ppb for the detection limit. The standard calibration profiles of fluoroquinolones showed linear responses to HPLC, showing more than 0.995 for the mean r(2) value. This is the first report of the simultaneous measurement of fluoroquinolones in eggs by a combination of SFE and HPLC. Using the SFE method allowed us to avoid extensive sample preparation such as solvent extraction and chromatographic cleanup that are basically required in extraction of fluoroquinolones.

show abstract

The in-vitro activity of a new highly active quinolone, DU-6859a

Cited by 24 publications

References 0 publications

In vitro activity of sitafloxacin against clinical strains of Streptococcus pneumoniae with defined amino acid substitutions in QRDRs of gyrase A and topoisomerase IV

In vitro activity of sitafloxacin against clinical strains of Streptococcus pneumoniae with defined amino acid substitutions in QRDRs of gyrase A and topoisomerase IV

Comparison of drug sensitivity and genotypes of clinically isolated strains of levofloxacin-resistant Streptococcus pneumoniae obtained from Okinawa Island, the Japanese main island and Hong Kong

Simultaneous Measurement of Fluoroquinolones in Eggs by a Combination of Supercritical Fluid Extraction and High Pressure Liquid Chromatography

Contact Info

Product

Resources

About