The in situ bacterial production of fluorescent organic matter; an investigation at a species level

Fox, Bethany; Thorn, Robin; Anesio, Alexandre M.; Reynolds, Darren M.

doi:10.1016/j.watres.2017.08.040

Cited by 94 publications

(94 citation statements)

References 43 publications

(51 reference statements)

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“…Figure shows the assignment of areas in an EEM for detectable substance groups. Tryptophan‐like substances are visible at 280/350–360 nm (Ex/Em), while tyrosine‐like substances appear in a region of 270–280/305–310 nm (Ex/Em) . The investigation of this study focused on the tryptophan content in the extracted bound EPS of AGS and SAS samples.…”

Section: Methodsmentioning

confidence: 99%

Anaerobic biodegradation and dewaterability of aerobic granular sludge

Jahn

Saracevic

Svardal

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Although a growing number of full‐scale wastewater treatment plants have already been constructed and operated with aerobic granular sludge (AGS), only limited information is available about further post‐treatment, in particular about sludge stabilization and dewaterability. The aim of the present study was to investigate the biodegradation and methane yield of AGS by the use of anaerobic laboratory‐scale reactors operated under mesophilic conditions and hydraulic retention times of 25 and 40 days. RESULTS The methane yield of AGS was ca 260 mL gVSS−1 (volatile suspended solids) and thus slightly increased compared to that of suspended activated sludge (SAS; 240 mL gVSS−1). A clear difference between the methane yield was found for separated pure granules (500 μm), which was ca 50% higher compared to that for SAS. VSS removal of AGS during anaerobic degradation was ca 52%. Dewaterability of AGS after anaerobic digestion was slightly lower compared to SAS. Extracellular polymeric substance (EPS) extraction and fluorescence analysis showed tryptophan contents which were almost twice as high compared to the EPS extracted from SAS. CONCLUSIONS Overall, the anaerobic digestion of AGS was found to be a suitable stabilization strategy with the benefit of recovering energy in the form of methane. Further tests are needed to validate the decreased dewatering behaviour with full‐scale applications. The presented approach for tryptophan measurement allows the transfer of qualitative results from a fluorescence analysis into quantitative values and could be further adapted for identifying relevant EPS constituents. © 2019 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Methodsmentioning

confidence: 99%

Anaerobic biodegradation and dewaterability of aerobic granular sludge

Jahn

Saracevic

Svardal

et al. 2019

J of Chemical Tech & Biotech

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“…The inoculum was added to individual sample bottles at a final concentration of 0.5% (v/v). The use of this minimal sample matrix, as opposed to a nutrient-rich media, made it possible to remove the interference that was caused by background fluorescence [6,9], while still providing sufficient nutrients to support bacterial growth. 'Supplemented synthetic water samples' were prepared by addition of 2% (v/v) environmental water to 'synthetic water samples' as a source of OM.…”

Section: Synthetic Water Samples and Supplemented Synthetic Water Sammentioning

confidence: 99%

“…Organic matter (OM) is ubiquitous to natural waters and it is a complex heterogeneous mixture that plays an important role in the transport of nutrients within global biogeochemical cycles [1][2][3]. Aquatic OM is complex, and it enters fluvial systems via a variety of sources, including that which has been transported into the system from terrestrial environments (allochthonous) and OM that has been generated in-situ (autochthonous) [4][5][6]. The molecular characteristics of aquatic OM are known to be complex and largely influenced by the biogeochemical processes that occur within the surrounding environment [2,7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Recently published literature has used the monoculturing of bacterial species to demonstrate the production and development of bacterially-produced organic matter over time, with a range of AFOM identified to be of microbial origin [6]. Whilst this work has contributed to the underpinning knowledge of the bacterial production and the processing of AFOM, there is a need for further studies to be undertaken which seek to understand the impacts of in situ processing, an essential parameter to consider when observing the biogeochemical cycling of organic material.…”

Section: Introductionmentioning

confidence: 99%

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Microbial Processing and Production of Aquatic Fluorescent Organic Matter in a Model Freshwater System

Fox

Thorn

Anesio

et al. 2018

Water

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Organic matter (OM) has an essential biogeochemical influence along the hydrological continuum and within aquatic ecosystems. Organic matter derived via microbial processes was investigated within a range of model freshwater samples over a 10-day period. For this, excitation-emission matrix (EEM) fluorescence spectroscopy in combination with parallel factor (PARAFAC) analysis was employed. This research shows the origin and processing of both protein-like and humic-like fluorescence within environmental and synthetic samples over the sampling period. The microbial origin of Peak T fluorescence is demonstrated within both synthetic samples and in environmental samples. Using a range of incubation temperatures provides evidence for the microbial metabolic origin of Peak T fluorescence. From temporally resolved experiments, evidence is provided that Peak T fluorescence is an indication of metabolic activity at the microbial community level and not a proxy for bacterial enumeration. This data also reveals that humic-like fluorescence can be microbially derived in situ and is not solely of terrestrial origin, likely to result from the upregulation of cellular processes prior to cell multiplication. This work provides evidence that freshwater microbes can engineer fluorescent OM, demonstrating that microbial communities not only process, but also transform, fluorescent organic matter.

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“…This water is regularly checked for its chemical and bacteriological quality. Only small number of inhabitants of this village uses water from individual wells the safety of which is not ensured (Fox et al, 2017). Farm 1 located next to this village is oriented on keeping sheep and goats (about 60).…”

Section: Monitored Area and Collection Of Samplesmentioning

confidence: 99%

Pollution of Surface and Ground Water by Sources Related to Agricultural Activities

Sasáková

Gregová

Takáčová

et al. 2018

Front. Sustain. Food Syst.

134

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The objective of this study was to monitor the quality of ground water supplied to animal farms and 2 villages and of surface water (rivers) in the same area (Košice basin, eastern Slovakia) with the aim to assess contamination of water by potential sources in this area. Samples for physico-chemical and microbiological examination were collected at 12 sampling points (6-surface water; 6-ground water) from May 2014 up to March 2015, covering all four seasons. The examination and evaluation of individual parameters was carried out according to relevant Slovak legislation compatible with EU Drinking water directive. The physico-chemical evaluation focused on parameters that indicate pollution of water resulting from human activities and farming. Microbiological examination included determination of counts of bacteria cultivated at 22 • C and 37 • C (BC22 and BC37), total coliforms, E. coli and fecal streptococci. Ground water intended for mass consumption (farms, villages) is abstracted from wells, collected in storage reservoirs and disinfected before brought to consumers. Some families in the villages use their own wells. Water for individual consumption (individual households) originates directly from individual wells. Examination of potable water used on agricultural farms showed some possibility of contamination of sources by runoff and inappropriate manipulation with excrements. Surface water in in the area close to both farms was polluted with organic substances (COD Mn ), however they did not exceed the limit set for surface water. At many samplings we detected in surface water presence of total coliforms, E. coli and occasionally also fecal enterococci indicating fecal pollution that could eventually affect ground water in individual wells. Our investigations showed that protection zones of water sources were not always sufficient. There were considerable variations in the quality of surface water during the year but no clear relationship between microbial contamination and seasons was observed. Quality of ground water supplied for mass consumption complied with legislative regulations except for BC 22 (heterotrophic count at 22 • C) in summer and autumn). Water from individual wells contained occasionally presence of total coliforms, E. coli and enterococci and higher heterotrophic counts.

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The in situ bacterial production of fluorescent organic matter; an investigation at a species level

Cited by 94 publications

References 43 publications

Anaerobic biodegradation and dewaterability of aerobic granular sludge

Anaerobic biodegradation and dewaterability of aerobic granular sludge

Microbial Processing and Production of Aquatic Fluorescent Organic Matter in a Model Freshwater System

Pollution of Surface and Ground Water by Sources Related to Agricultural Activities

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