The Improvement of Immune Effect of Recombinant Human Beta-Defensin 2 on Hepatitis B Vaccine in Mice

Wang, Meizhen; Zeng, Weizhong; Zhang, Zhigang; Zhang, Weidong; Su, Hong; Zhang, Zhiyong; Jiang, Lixia; Liu, Yulin; Shi, Qiaofa

doi:10.1089/vim.2020.0052

Cited by 5 publications

(3 citation statements)

References 58 publications

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“…Moreover, it induces the Th1 immune response which is essential for the clearance of intracellular pathogens. Furthermore, this adjuvant is safe and well-tolerated as it is a part of the natural system of the human body that possesses safe morphology [ [63] , [64] , [65] ]. The molecular weight of the vaccine is 42 KDa, which supports the perfect range for a vaccine.…”

Section: Discussionmentioning

confidence: 99%

Structural vaccinology-based design of multi-epitopes vaccine against Streptococcus gordonii and validation using molecular modeling and immune simulation approaches

Nasir,

Iftikhar,

Zubair

et al. 2023

Heliyon

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Section: Discussionmentioning

confidence: 99%

Structural vaccinology-based design of multi-epitopes vaccine against Streptococcus gordonii and validation using molecular modeling and immune simulation approaches

Nasir,

Iftikhar,

Zubair

et al. 2023

Heliyon

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“…Defensin hBD-2 enhances the antigen-specific immune response not only against bacterial, but also toward viral antigens. The introduction of hBD-2 defensin into the vaccine increases the immunogenicity of vaccines against hepatitis B [ 138 ] and hepatitis C, both in free [ 139 ] and conjugated with a polypeptide form [ 140 ]. hBD-2 is being introduced as an adjuvant in MERS-CoV vaccines under development [ 141 , 142 , 143 ].…”

Section: Immunomodulatory Activity Of Ampsmentioning

confidence: 99%

Immunomodulatory and Allergenic Properties of Antimicrobial Peptides

Guryanova

Ovchinnikova

2022

IJMS

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With the growing problem of the emergence of antibiotic-resistant bacteria, the search for alternative ways to combat bacterial infections is extremely urgent. While analyzing the effect of antimicrobial peptides (AMPs) on immunocompetent cells, their effect on all parts of the immune system, and on humoral and cellular immunity, is revealed. AMPs have direct effects on neutrophils, monocytes, dendritic cells, T-lymphocytes, and mast cells, participating in innate immunity. They act on B-lymphocytes indirectly, enhancing the induction of antigen-specific immunity, which ultimately leads to the activation of adaptive immunity. The adjuvant activity of AMPs in relation to bacterial and viral antigens was the reason for their inclusion in vaccines and made it possible to formulate the concept of a “defensin vaccine” as an innovative basis for constructing vaccines. The immunomodulatory function of AMPs involves their influence on cells in the nearest microenvironment, recruitment and activation of other cells, supporting the response to pathogenic microorganisms and completing the inflammatory process, thus exhibiting a systemic effect. For the successful use of AMPs in medical practice, it is necessary to study their immunomodulatory activity in detail, taking into account their pleiotropy. The degree of maturity of the immune system and microenvironment can contribute to the prevention of complications and increase the effectiveness of therapy, since AMPs can suppress inflammation in some circumstances, but aggravate the response and damage of organism in others. It should also be taken into account that the real functions of one or another AMP depend on the types of total regulatory effects on the target cell, and not only on properties of an individual peptide. A wide spectrum of biological activity, including direct effects on pathogens, inactivation of bacterial toxins and influence on immunocompetent cells, has attracted the attention of researchers, however, the cytostatic activity of AMPs against normal cells, as well as their allergenic properties and low stability to host proteases, are serious limitations for the medical use of AMPs. In this connection, the tasks of searching for compounds that selectively affect the target and development of an appropriate method of application become critically important. The scope of this review is to summarize the current concepts and newest advances in research of the immunomodulatory activity of natural and synthetic AMPs, and to examine the prospects and limitations of their medical use.

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“…In many studies, several kinds of adjuvants were used that had significant effects on the immunogenicity of the vaccine (Cayatte et al 2017 ; Doener et al 2019 ; Gasparini et al 2001 ; Suschak et al 2017 ; Yang et al 2020 ). Adjuvants L7/L12 ribosomal protein (Behbahani 2020 ; Saadi et al 2017 ), beta-defensin (Gupta et al 2020 ; Wang et al 2020 ), HBHA protein (Kumar et al 2019 ; Lei et al 2020 ), and HBHA conserved sequence(Rashidian et al 2020 ; Solanki and Tiwari 2018 ) recently been identified as efficient and helpful adjuvants, and we used them in this study.…”

Section: Introductionmentioning

confidence: 99%

Design of a Multi-epitope Vaccine Against Acinetobacter baumannii Using Immunoinformatics Approach

Touhidinia

Sefid

Bidakhavidi

2021

Int J Pept Res Ther

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Acinetobacter baumannii is one of the most successful pathogens causing nosocomial infections and has significantly multidrug-resistant. So far, there are no certain treatments to protect against infection with A. baumannii , therefore an effective A. baumannii vaccine needed. The purpose of this study was to predict antigenic epitopes of CarO protein for designing the A. baumannii vaccine using immunoinformatics analysis. CarO protein is one of the most important factors in the resistance against the antibiotic Carbapenem . In this study, T and B-cell epitopes of CarO protein were predicted and screened based on the antigenicity, toxicity, allergenicity features. The epitopes were linked by suitable linkers. Four different adjuvants were attached to the vaccine constructs which among them, vaccine construct 3 was chosen to predict the secondary and the 3D structure of the vaccine. The refinement process was performed to improve the quality of the 3D model structure; the validation process is performed using the Ramachandran plot and ProSA z-score. The designed vaccine's binding affinity to six various HLA molecules and TLR 2 and TLR4 were evaluated by molecular docking. Finally, in silico gene cloning was performed in the pET28a (+) vector. The findings suggest that the vaccine may be a promising vaccine to prevent A. baumannii infection.

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The Improvement of Immune Effect of Recombinant Human Beta-Defensin 2 on Hepatitis B Vaccine in Mice

Cited by 5 publications

References 58 publications

Structural vaccinology-based design of multi-epitopes vaccine against Streptococcus gordonii and validation using molecular modeling and immune simulation approaches

Structural vaccinology-based design of multi-epitopes vaccine against Streptococcus gordonii and validation using molecular modeling and immune simulation approaches

Immunomodulatory and Allergenic Properties of Antimicrobial Peptides

Design of a Multi-epitope Vaccine Against Acinetobacter baumannii Using Immunoinformatics Approach

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