The importin α subunit PsIMPA1 mediates the oxidative stress response and is required for the pathogenicity of Phytophthora sojae

Yang, Xinyu; Ding, Fa; Zhang, Lei; Sheng, Yuting; Zheng, Xiaobo; Wang, Yuanchao

doi:10.1016/j.fgb.2015.04.023

Cited by 11 publications

(6 citation statements)

References 48 publications

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“…These seed nodes are well predicted to be pathogenicity proteins, implying that UV_2443 might be an important pathogenicity protein. This speculation is consistent with the previous finding that PsIMPA1, sharing 41.8% identity with UV_2443, was demonstrated to be required for Phytophthora sojae pathogenicity . The PsIMPA1 mutants exhibited a decreased growth rate and could not form sporangia nor release zoospores.…”

Section: Discussionsupporting

confidence: 92%

“…This speculation is consistent with the previous finding that PsIMPA1, sharing 41.8% identity with UV_2443, was demonstrated to be required for Phytophthora sojae pathogenicity. 84 The PsIMPA1 mutants exhibited a decreased growth rate and could not form sporangia nor release zoospores. Similarly, several protein kinases, such as UV_7103 (histidine kinase 5p), UV_6310 (CDC28), UV_1267 (G-protein alpha subunit GPA1), and UV_2494 (Fus3/Kss1), had three seed partners.…”

Section: Journal Of Proteome Researchmentioning

confidence: 98%

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Pathogenicity Genes in Ustilaginoidea virens Revealed by a Predicted Protein–Protein Interaction Network

Zhang

et al. 2017

J. Proteome Res.

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Rice false smut, caused by Ustilaginoidea virens, produces significant losses in rice yield and grain quality and has recently emerged as one of the most important rice diseases worldwide. Despite its importance in rice production, relatively few studies have been conducted to illustrate the complex interactome and the pathogenicity gene interactions. Here a protein-protein interaction network of U. virens was built through two well-recognized approaches, interolog- and domain-domain interaction-based methods. A total of 20 217 interactions associated with 3305 proteins were predicted after strict filtering. The reliability of the network was assessed computationally and experimentally. The topology of the interactome network revealed highly connected proteins. A pathogenicity-related subnetwork involving up-regulated genes during early U. virens infection was also constructed, and many novel pathogenicity proteins were predicted in the subnetwork. In addition, we built an interspecies PPI network between U. virens and Oryza sativa, providing new insights for molecular interactions of this host-pathogen pathosystem. A web-based publicly available interactive database based on these interaction networks has also been released. In summary, a proteome-scale map of the PPI network was described for U. virens, which will provide new perspectives for finely dissecting interactions of genes related to its pathogenicity.

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Section: Discussionsupporting

confidence: 92%

Section: Journal Of Proteome Researchmentioning

confidence: 98%

Pathogenicity Genes in Ustilaginoidea virens Revealed by a Predicted Protein–Protein Interaction Network

Zhang

et al. 2017

J. Proteome Res.

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“…Few studies have addressed molecular aspects of the development of sexual spores in oomycetes, in contrast to the more extensive literature on their asexual spores. Several genes influencing oospore formation have been identified in Phytophthora , but they also affect general growth and their impact on oosporogenesis may be secondary [ 61 – 63 ]. One exception comes from the oomycete Py .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and proteomic analysis reveals wall-associated and glucan-degrading proteins with potential roles in Phytophthora infestans sexual spore development

et al. 2018

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Sexual reproduction remains an understudied feature of oomycete biology. To expand our knowledge of this process, we used RNA-seq and quantitative proteomics to examine matings in Phytophthora infestans. Exhibiting significant changes in mRNA abundance in three matings between different A1 and A2 strains compared to nonmating controls were 1170 genes, most being mating-induced. Rising by >10-fold in at least one cross were 455 genes, and 182 in all three crosses. Most genes had elevated expression in a self-fertile strain. Many mating-induced genes were associated with cell wall biosynthesis, which may relate to forming the thick-walled sexual spore (oospore). Several gene families were induced during mating including one encoding histidine, serine, and tyrosine-rich putative wall proteins, and another encoding prolyl hydroxylases which may strengthen the extracellular matrix. The sizes of these families vary >10-fold between Phytophthora species and one exhibits concerted evolution, highlighting two features of genome dynamics within the genus. Proteomic analyses of mature oospores and nonmating hyphae using isobaric tags for quantification identified 835 shared proteins, with 5% showing >2-fold changes in abundance between the tissues. Enriched in oospores were β-glucanases potentially involved in digesting the oospore wall during germination. Despite being dormant, oospores contained a mostly normal complement of proteins required for core cellular functions. The RNA-seq data generated here and in prior studies were used to identify new housekeeping controls for gene expression studies that are more stable than existing normalization standards. We also observed >2-fold variation in the fraction of polyA+ RNA between life stages, which should be considered when quantifying transcripts and may also be relevant to understanding translational control during development.

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“…For hosts, different importin α isoforms govern the host adaptation of influenza virus [74]. Some importin-related genes involved in oxidative stress response, pathogenicity and transport of cargoes were identified [75]. For example, the β-importin KAP8 (Pse1/Kap121) is required for nuclear import of the cellulose transcriptional regulator XYR1, asexual sporulation, and stress resistance in Trichoderma reesei [76].…”

Section: Functions Of the Genes Related To Host Adaptationmentioning

confidence: 99%

Comparative Genomics and Gene Pool Analysis Reveal the Decrease of Genome Diversity and Gene Number in Rice Blast Fungi by Stable Adaption with Rice

Wang

Liu

et al. 2021

JoF

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Magnaporthe oryzae caused huge losses in rice and wheat production worldwide. Comparing to long-term co-evolution history with rice, wheat-infecting isolates were new-emerging. To reveal the genetic differences between rice and wheat blast on global genomic scale, 109 whole-genome sequences of M. oryzae from rice, wheat, and other hosts were reanalyzed in this study. We found that the rice lineage had gone through stronger selective sweep and fewer conserved genes than those of Triticum and Lolium lineages, which indicated that rice blast fungi adapted to rice by gene loss and rapid evolution of specific loci. Furthermore, 228 genes associated with host adaptation of M. oryzae were found by presence/absence variation (PAV) analyses. The functional annotation of these genes found that the fine turning of genes gain/loss involved with transport and transcription factor, thiol metabolism, and nucleotide metabolism respectively are major mechanisms for rice adaption. This result implies that genetic base of specific host plant may lead to gene gain/loss variation of pathogens, so as to enhance their adaptability to host. Further characterization of these specific loci and their roles in adaption and evaluation of the fungi may eventually lead to understanding of interaction mechanism and develop new strategies of the disease management.

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The importin α subunit PsIMPA1 mediates the oxidative stress response and is required for the pathogenicity of Phytophthora sojae

Cited by 11 publications

References 48 publications

Pathogenicity Genes in Ustilaginoidea virens Revealed by a Predicted Protein–Protein Interaction Network

Pathogenicity Genes in Ustilaginoidea virens Revealed by a Predicted Protein–Protein Interaction Network

Transcriptomic and proteomic analysis reveals wall-associated and glucan-degrading proteins with potential roles in Phytophthora infestans sexual spore development

Comparative Genomics and Gene Pool Analysis Reveal the Decrease of Genome Diversity and Gene Number in Rice Blast Fungi by Stable Adaption with Rice

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