The important role of tricarboxylic acid cycle metabolism pathways and core bacterial communities in carbon sequestration during chicken manure composting

Wang, Liqin; Qu, Fengting; Zhu, Zechen; Zhao, Yue; Chen, Xiaomeng; Shi, Mingzi; Wei, Zimin

doi:10.1016/j.wasman.2022.06.034

Cited by 15 publications

(3 citation statements)

References 42 publications

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“…The citric acid cycle is an important aerobic pathway that is the final step in the metabolism of sugar, fat, and amino acids. 42 In a series of reactions, the two carbons in citrate are oxidized to carbon dioxide, and this reaction pathway provides NADH for oxidative phosphorylation and other metabolic processes. After treating S. aureus with BY2, the expression of 15 genes involved in the citric acid cycle was down-regulated.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Thesium chinense Turcz Extract Against Bacteria Associated with Upper Respiratory Tract Infections

Wei,

Zhang,

et al. 2023

IDR

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Purpose The drug resistance of Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes and Haemophilus influenzae has become more and more serious, and it is urgent to seek new antibacterial drugs. In this study, Thesium chinense Turcz. extracts were tested for its potential antibacterial activities. Methods T. chinense powder was extracted with 5 solvents of different polarity (ethyl alcohol, petroleum ether, ethyl acetate, n-butyl alcohol and double distilled water), and their antibacterial activities were tested. The Broth dilution method was used to evaluate the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of highly active plant extracts with a concentration of 1g/mL. The inhibitory activity of this extract on biofilm formation was investigated. Afterwards, we investigated its effect on the transcriptome of S. aureus . Results The ethanol extract coded as BRY, only inhibited S. aureus , whereas the ethyl acetate extract coded as BY2 showed inhibitory effect on all the tested bacteria. The MIC of BRY on S. aureus was 128 mg/mL, and the MBC was 512 mg/mL. The MIC of BY2 against S. aureus, S. pneumoniae, S. pyogenes and H. influenzae were 8 mg/mL, 4 mg/mL, 4 mg/mL, and 4 mg/mL, respectively. The MBC of BY2 for these four bacteria ranged from 4 to 256 mg/mL. Mechanism studies have shown that BRY and BY2 have an impact on anti-formation of biofilms at MIC concentrations. Transcriptome sequencing results showed that 531 genes were up-regulated and 340 genes showed down-regulated expression in S. aureus after BY2 treatment. Conclusion BY2 has a broader antibacterial spectrum than BRY. Meanwhile, the inhibitory effect of BY2 on S. aureus is better than BRY. The mechanism of BY2 against S. aureus may relate to its inhibition of ribosome synthesis, restriction of key enzymes of citric acid cycle, decrease of pathogenicity and influence on biofilm formation. The results confirmed that BY2 was the main antibacterial part of T. chinense , which can be used as a source of antibacterial agents.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Thesium chinense Turcz Extract Against Bacteria Associated with Upper Respiratory Tract Infections

Wei,

Zhang,

et al. 2023

IDR

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“…Interestingly, the level of L-proline in tetraploid rice decreased with the development of rice endosperm, while the content of L-proline in diploid rice increased first and then decreased, which may indicate that the initial and maximum accumulation times of metabolites differed among materials. The TCA cycle is a ubiquitous metabolic pathway in aerobic organisms ( Dijkstra et al., 2011 ; Wang et al., 2022b ). Fumarate and succinate decreased with endosperm development, while citrate increased with endosperm development in tetraploid rice and decreased first and then increased in diploid rice ( Figure 7 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics and transcriptomics analyses provide new insights into the nutritional quality during the endosperm development of different ploidy rice

et al. 2023

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Autotetraploid rice is developed from diploid rice by doubling the chromosomes, leading to higher nutritional quality. Nevertheless, there is little information about the abundances of different metabolites and their changes during endosperm development in autotetraploid rice. In this research, two different kinds of rice, autotetraploid rice (AJNT-4x) and diploid rice (AJNT-2x), were subjected to experiments at various time points during endosperm development. A total of 422 differential metabolites, were identified by applying a widely used metabolomics technique based on LC-MS/MS. KEGG classification and enrichment analysis showed the differences in metabolites were primarily related to biosynthesis of secondary metabolites, microbial metabolism in diverse environments, biosynthesis of cofactors, and so on. Twenty common differential metabolites were found at three developmental stages of 10, 15 and 20 DAFs, which were considered the key metabolites. To identify the regulatory genes of metabolites, the experimental material was subjected to transcriptome sequencing. The DEGs were mainly enriched in starch and sucrose metabolism at 10 DAF, and in ribosome and biosynthesis of amino acids at 15 DAF, and in biosynthesis of secondary metabolites at 20 DAF. The numbers of enriched pathways and the DEGs gradually increased with endosperm development of rice. The related metabolic pathways of rice nutritional quality are cysteine and methionine metabolism, tryptophan metabolism, lysine biosynthesis and histidine metabolism, and so on. The expression level of the genes regulating lysine content was higher in AJNT-4x than in AJNT-2x. By applying CRISPR/Cas9 gene-editing technology, we identified two novel genes, OsLC4 and OsLC3, negatively regulated lysine content. These findings offer novel insight into dynamic metabolites and genes expression variations during endosperm development of different ploidy rice, which will aid in the creation of rice varieties with better grain nutritional quality.

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“…are capable of obtaining phosphate from phosphate rock (PR) to promote aerobic rice growth [143]. The tricarboxylic acid cycle (TCA cycle) is the main pathway leading to the production of acids, which is the core energy source and a prerequisite for the synthesis of biological compounds in living organisms [144][145][146]. The TCA cycle is also present in bacteria, and the acids secreted by it can efficiently dissolve insoluble phosphorus.…”

Section: Bacteria Convert Soil Phosphorus Which Helps Plants To Effec...mentioning

confidence: 99%

Influence and Role of Fungi, Bacteria, and Mixed Microbial Populations on Phosphorus Acquisition in Plants

Luo,

Ma,

Feng

et al. 2024

Agriculture

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Phosphorus (P) stands as a pivotal macroelement in relation to the growth of plants. It plays a significant role in physiological processes, as components of biofilms and nucleotides, and in metabolic activities within plants. The deprivation of phosphorus detrimentally impacts the growth and developmental of plants. However, the rhizosphere’s beneficial fungi and bacteria augment the efficacy of phosphorus uptake, participate in the molecular regulation of phosphorus, stimulate physiological alterations in plants, and facilitate signal transmission. In order to give readers a better understanding of the effects and positive roles of soil beneficial fungi and bacteria in regulating plant phosphorus acquisition and transport, this present review introduces the role and influence of rhizosphere microorganisms (fungi and bacteria) in assisting plant phosphorus absorption, and summarizes the key phosphorus transporters found in their interaction with plants. Using mixed microbial populations as composite microbial fertilizers has a positive effect on plants under phosphorus-deficiency conditions. It will be conducive to a better understanding of the mutualistic relationship between fungi, bacteria, and plants to provide a way to reduce the application of phosphorus fertilizers efficiently, and to provide a research background for the development of microbiological fertilizers.

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The important role of tricarboxylic acid cycle metabolism pathways and core bacterial communities in carbon sequestration during chicken manure composting

Cited by 15 publications

References 42 publications

Antibacterial Activity of Thesium chinense Turcz Extract Against Bacteria Associated with Upper Respiratory Tract Infections

Antibacterial Activity of Thesium chinense Turcz Extract Against Bacteria Associated with Upper Respiratory Tract Infections

Metabolomics and transcriptomics analyses provide new insights into the nutritional quality during the endosperm development of different ploidy rice

Influence and Role of Fungi, Bacteria, and Mixed Microbial Populations on Phosphorus Acquisition in Plants

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