The importance of selecting a proper biological milieu for protein corona analysis in vitro: Human plasma versus human serum

Mirshafiee, Vahid; Kim, Raehyun; Mahmoudi, Morteza; Kraft, Mary L.

doi:10.1016/j.biocel.2015.11.019

Cited by 123 publications

(91 citation statements)

References 56 publications

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“…93 To enhance the precise prediction of in vivo biological outcomes and practical applications of protein corona in pharmaceutical sciences, initially a full assessment of NP-protein interaction in stimulated biomimetic administration conditions must be done. 109 Specifically, media exposure factors including proteins, fluidic shear stress, and also degradable enzymes should be carefully set up. Besides, the invention of new pharmaceutical technologies such as tumor-on-a-chip models and biomimetic microfluidic systems allow for more deeper evaluation of NP behaviors.…”

Section: Future Perspectivesmentioning

confidence: 99%

Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

522

343

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After administration of nanoparticle (NP) into biological fluids, an NP–protein complex is formed, which represents the “true identity” of NP in our body. Hence, protein–NP interaction should be carefully investigated to predict and control the fate of NPs or drug-loaded NPs, including systemic circulation, biodistribution, and bioavailability. In this review, we mainly focus on the formation of protein corona and its potential applications in pharmaceutical sciences such as prediction modeling based on NP-adsorbed proteins, usage of active proteins for modifying NP to achieve toxicity reduction, circulation time enhancement, and targeting effect. Validated correlative models for NP biological responses mainly based on protein corona fingerprints of NPs are more highly accurate than the models solely set up from NP properties. Based on these models, effectiveness as well as the toxicity of NPs can be predicted without in vivo tests, while novel cell receptors could be identified from prominent proteins which play important key roles in the models. The ungoverned protein adsorption onto NPs may have generally negative effects such as rapid clearance from the bloodstream, hindrance of targeting capacity, and induction of toxicity. In contrast, controlling protein adsorption by modifying NPs with diverse functional proteins or tailoring appropriate NPs which favor selective endogenous peptides and proteins will bring promising therapeutic benefits in drug delivery and targeted cancer treatment.

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Section: Future Perspectivesmentioning

confidence: 99%

Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

522

343

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“…Proteins involved in the physiological as well as toxicological relevant processes in blood, such as complement activation and coagulation have been identified in coronas of various NP (Docter et al, 2015;. Human plasma comprising 55% of the blood volume was used for biocorona studies, since it is more representative of the physiological fluid than serum, which lacks clotting factors and fibrinogen (Mirshafiee et al, 2016). Consistent with our data, existing literature on corona proteins over AuNP have identified albumin, apolipoprotein, immunoglobulins, complement and fibrinogen as the most abundantly bound species (Karmali & Simberg, 2011;Lacerda et al, 2010;Nel et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Surface chemistry of gold nanoparticles determines the biocorona composition impacting cellular uptake, toxicity and gene expression profiles in human endothelial cells

2017

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This study investigated the role of nanoparticle size and surface chemistry on biocorona composition and its effect on uptake, toxicity and cellular responses in human umbilical vein endothelial cells (HUVEC), employing 40 and 80 nm gold nanoparticles (AuNP) with branched polyethyleneimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coatings. Proteomic analysis identified 59 hard corona proteins among the various AuNP, revealing largely surface chemistry-dependent signature adsorbomes exhibiting human serum albumin (HSA) abundance. Size distribution analysis revealed the relative instability and aggregation inducing potential of bare and corona-bound BPEI-AuNP, over LA-and PEG-AuNP. Circular dichroism analysis showed surface chemistry-dependent conformational changes of proteins binding to AuNP. Timedependent uptake of bare, plasma corona (PC) and HSA corona-bound AuNP (HSA-AuNP) showed significant reduction in uptake with PC formation. Cell viability studies demonstrated dose-dependent toxicity of BPEI-AuNP. Transcriptional profiling studies revealed 126 genes, from 13 biological pathways, to be differentially regulated by 40 nm bare and PC-bound BPEI-AuNP (PC-BPEI-AuNP). Furthermore, PC formation relieved the toxicity of cationic BPEI-AuNP by modulating expression of genes involved in DNA damage and repair, heat shock response, mitochondrial energy metabolism, oxidative stress and antioxidant response, and ER stress and unfolded protein response cascades, which were aberrantly expressed in bare BPEI-AuNP-treated cells. NP surface chemistry is shown to play the dominant role over size in determining the biocorona composition, which in turn modulates cell uptake, and biological responses, consequently defining the potential safety and efficacy of nanoformulations. ARTICLE HISTORY

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“…Although relevant, factors defined by NPs are fewer compared to media-dependent factors. Composition of the NPs corona depends on the source of proteins [29–31], time [9,32] and temperature [33] of the incubation of NPs in biological fluids. Not all variables are so intuitive; corona composition can also be affected by serum heat inactivation [34], choice of anticoagulant [30], static versus dynamic conditions of the media [35] and even the presence of magnetic field in the case of iron oxide NPs [36].…”

Section: Introductionmentioning

confidence: 99%

Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona

et al. 2017

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Protein corona of nanoparticles (NPs), which forms when these particles come in to contact with protein-containing fluids, is considered as an overlooked factor in nanomedicine. Through numerous studies it has been becoming increasingly evident that it importantly dictates the interaction of NPs with their surroundings. Several factors that determine the compositions of NPs protein corona have been identified in recent years, but one has remained largely ignored—the composition of media used for dispersion of NPs. Here, we determined the effect of dispersion media on the composition of protein corona of polyacrylic acid-coated cobalt ferrite NPs (PAA NPs) and silica NPs. Our results confirmed some of the basic premises such as NPs type-dependent specificity of the protein corona. But more importantly, we demonstrated the effect of the dispersion media on the protein corona composition. The differences between constituents of the media used for dispersion of NPs, such as divalent ions and macromolecules were responsible for the differences in protein corona composition formed in the presence of fetal bovine serum (FBS). Our results suggest that the protein corona composition is a complex function of the constituents present in the media used for dispersion of NPs. Regardless of the dispersion media and FBS concentration, majority of proteins from either PAA NPs or silica NPs coronas were involved in the process of transport and hemostasis. Interestingly, corona of silica NPs contained three complement system related proteins: complement factor H, complement C3 and complement C4 while PAA NPs bound only one immune system related protein, α-2-glycoprotein. Importantly, relative abundance of complement C3 protein in corona of silica NPs was increased when NPs were dispersed in NaCl, which further implies the relevance of dispersion media used to prepare NPs.

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The importance of selecting a proper biological milieu for protein corona analysis in vitro: Human plasma versus human serum

Cited by 123 publications

References 56 publications

Protein corona: a new approach for nanomedicine design

Protein corona: a new approach for nanomedicine design

Surface chemistry of gold nanoparticles determines the biocorona composition impacting cellular uptake, toxicity and gene expression profiles in human endothelial cells

Dispersion of Nanoparticles in Different Media Importantly Determines the Composition of Their Protein Corona

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