The importance of porcine sperm parameters on fertility in vivo

Tardif, Steve; Laforest, Jean-Paul; Cormier, Nathaly; Bailey, Janice L.

doi:10.1016/s0093-691x(99)00142-9

Cited by 114 publications

(51 citation statements)

References 28 publications

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“…The sperm were incubated in Cap medium, and capacitation was induced, as assessed by the CTCfluorescence staining pattern of the sperm head (Wang et al 1995, Tardif et al 1999. Under the conditions of the present experiment, over 70% of the sperm represented pattern B fluorescence (fluorescence-free band in the postacrosomal region), thus proving them to be capacitated sperm (Fig.…”

Section: Evaluation Of Capacitation and Hyperactivationmentioning

confidence: 58%

Porcine sperm capacitation involves tyrosine phosphorylation and activation of aldose reductase

Katoh¹,

Takebayashi²,

Kikuchi³

et al. 2014

Reproduction

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Mammalian sperm must be activated in the tubal isthmus through capacitation to induce the acrosome reaction and subsequent fertilization. Although the molecular mechanisms involved in capacitation have yet to be fully elucidated, increased concentrations of reactive oxygen species (ROS) and the extent of tyrosine phosphorylation of proteins have been suggested to play central roles in the completion of capacitation. In this study, aldose reductase was for the first time identified as one of the tyrosine-phosphorylated proteins involved in the capacitation of porcine cauda epididymal sperm. Both tyrosine phosphorylation and activity of aldose reductase associated with the particulate fraction of sperm cells were significantly enhanced during capacitation. Alrestatin, a membranepermeable and specific inhibitor of aldose reductase, plays a role in the inhibition of aldose reductase activity, elevation of intracellular levels of ROS, and induction of hyperactivated motility, all at similar dose dependencies. Alrestatin canceled both the increase in the tyrosine phosphorylation of aldose reductase and the decrease in the glutathione levels in sperm-induced during capacitation. The hyperactivated motility was induced to a higher extent in the presence of glucose than in the presence of fructose. These results indicate that aldose reductase plays an important role in induction of hyperactivation and capacitation of sperm through the elevation of ROS in sperm cells. Furthermore, aldose reductase was shown to be added to sperm during transit through the epididymis, suggesting that aldose reductase is one of the key proteins that support the functional maturation of sperm.

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Section: Evaluation Of Capacitation and Hyperactivationmentioning

confidence: 58%

Porcine sperm capacitation involves tyrosine phosphorylation and activation of aldose reductase

Katoh¹,

Takebayashi²,

Kikuchi³

et al. 2014

Reproduction

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“…But there are certain variations in pig fertility explained by the boar and semen parameters. Many researchers have studied the relationship between semen parameters and fertility, with conflicting results (Tardif et al, 1999;Sellés et al, 2003;Popwell and Flowers, 2004;Ruiz-Sánchez et al, 2006;Broekhuijse et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variation in sperm characteristics of boars in southern Uruguay

2015

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-The objective of this study was to evaluate the effects of season, natural photoperiod, and room temperature at the housing facility on boar semen characteristics in Uruguay (34º66'S; 56º29'W). For this purpose, 117 ejaculates, obtained from eight adult males collected through 12 consecutive months, were assessed for sperm viability, DNA integrity, abnormalities (total, primary, and secondary), ejaculate volume, and sperm concentration. Viability, total and primary abnormalities, volume, and sperm concentration were affected by season. Sperm viability, volume, and sperm concentration were affected by natural photoperiod. In general, autumn and the decreasing photoperiod had a negative impact on most of the semen characteristics, except for volume. Housing temperature did not affect semen characteristics. In boars living in temperate climates, semen quality is negatively affected during autumn and is related to photoperiod changes; however, the effects of temperature changes in housingdo not affect these seminal characteristics. In this scenario, seasonal differences in semen quality may have a negative effect on sow fertilization. Consequently, semen quality control especially during autumn is imperative for the best boar selection to be used for insemination purposes. Seasonal differences in semen quality may have a negative effect on sow reproductive performance. This issue will be addressed in a future investigation.

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“…There are likely additional types of cellular damage due to cryopreservation that are not detected in standard semen analysis, given the low correlation between fertility and traditional microscopic evaluation. Numerous attempts have been made to identify in vitro sperm parameters that that are directly related to fertility (Holt et al, 1997;Tardif et al, 1999;Larsson & Rodrıǵuez-Martıńez, 2000;Waberski et al, 2005;Alm et al, 2006;Broekhuijse et al, 2012;Schulze et al, 2013). However, novel assays that measure sperm interactions within the female reproductive tract and with oocytes may be more predictive of in vivo function as well as useful when identifying reasons for reduced fertility (Fazeli et al, 1997;Braundmeier et al, 2004;Collins et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

Daigneault

McNamara

Purdy³

et al. 2015

Andrology

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SUMMARYDue to reduced fertility, cryopreserved semen is seldom used for commercial porcine artificial insemination (AI). Predicting the fertility of individual frozen ejaculates for selection of higher quality semen prior to AI would increase overall success. Our objective was to test novel and traditional laboratory analyses to identify characteristics of cryopreserved spermatozoa that are related to boar fertility. Traditional post-thaw analyses of motility, viability, and acrosome integrity were performed on each ejaculate. In vitro fertilization, cleavage, and blastocyst development were also determined. Finally, spermatozoa-oviduct binding and competitive zonabinding assays were applied to assess sperm adhesion to these two matrices. Fertility of the same ejaculates subjected to laboratory assays was determined for each boar by multi-sire AI and defined as (i) the mean percentage of the litter sired and (ii) the mean number of piglets sired in each litter. Means of each laboratory evaluation were calculated for each boar and those values were applied to multiple linear regression analyses to determine which sperm traits could collectively estimate fertility in the simplest model. The regression model to predict the percent of litter sired by each boar was highly effective (p < 0.001, r 2 = 0.87) and included five traits;acrosome-compromised spermatozoa, percent live spermatozoa (0 and 60 min post-thaw), percent total motility, and the number of zona-bound spermatozoa. A second model to predict the number of piglets sired by boar was also effective (p < 0.05, r 2 = 0.57).These models indicate that the fertility of cryopreserved boar spermatozoa can be predicted effectively by including traditional and novel laboratory assays that consider functions of spermatozoa.

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The importance of porcine sperm parameters on fertility in vivo

Cited by 114 publications

References 28 publications

Porcine sperm capacitation involves tyrosine phosphorylation and activation of aldose reductase

Porcine sperm capacitation involves tyrosine phosphorylation and activation of aldose reductase

Seasonal variation in sperm characteristics of boars in southern Uruguay

Enhanced fertility prediction of cryopreserved boar spermatozoa using novel sperm function assessment

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